A multiplexed set of microsatellite markers for discriminating Acacia mangium, A. auriculiformis, and their hybrid

Le, Son; Ratnam, R Wickneswari V; Harwood, CE; Larcombe, Matthew J.; Griffin, R; Koutoulis, Anthony; Harbard, J. L.; Cyer, Koh Sin; Yee, Liew Wai; Ha, Thinh H.; Vaillancourt, René E.

doi:10.1007/s11295-016-0990-2

Cited by 14 publications

(13 citation statements)

References 47 publications

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“…Thus, the development of a molecular marker set to establish the molecular pattern of each cultivar (fingerprinting) is extremely important to breeding programs. This cultivar discrimination must be fast, accurate, and precise (Collard et al 2005;Le et al 2016). Molecular markers have been increasingly used in recent times and is also important for the continuity of breeding programs and for the guarantee of the intellectual property of the final product of genetic selection (Ercisli et al 2011;Rauscher and Simko 2013;Scarano et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity

et al. 2017

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New cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes using morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/ progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars.

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Section: Introductionmentioning

confidence: 99%

Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity

et al. 2017

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“…Because of the threat posed by Ceratocystis, research recently commenced in Sabah, Indonesia and Vietnam to find Ceratocystis-tolerant planting material (Brawner 2017, Brawner et al 2015, Kien et al 2017. However, in comparison with other tree species, there is very little genetic variation within A. mangium (Moran et al 1989, Son et al 2016, thus screening wider collections of provenances for disease tolerance may not be fruitful. Acacia auriculiformis has higher genetic diversity as well as greater genetic tolerance to Ceratocystis, and some hybrids of A. mangium x A. auriculiformis have shown good tolerance to infection by Ceratocystis in Vietnam (Kien et al 2017) and in Sabah.…”

Section: Successes and Failuresmentioning

confidence: 99%

Observations on the Successes and Failures of Acacia Plantations in Sabah and Sarawak and the Way Forward

Lee¹

2018

JTFS

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Development of acacia plantations, mainly Acacia mangium, in Sabah and Sarawak has progressed well for over three decades. Despite some disease problems, A. mangium is still the preferred plantation species in Sabah and Sarawak as it is a fast-growing and flexible species that is able to grow on poor and degraded soils. However, reports of widespread mortality of young plantations due to root-rot diseases and Ceratocystis wilt, particularly in Indonesia, and fear of future failure of acacia plantations have caused alarm among plantation managers and growers, leading some quarters to call for a change of tree species for fastgrowing plantations. This paper discusses two of the major diseases affecting Acacia species, particularly A. mangium, the successes and failures of acacia plantations in Sabah and Sarawak, and the way forward.

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“…A total of 76 SSR markers were used in amplification analyses of A. melanoxylon. These included 15 primers previously used in Acacia mangium × A. auriculiformis) (Ng et al 2005), 33 employed in A. mangium , 20 used in A. auriculiformis × A. mangium (Le et al 2016), and 8 employed in Paraserianthes falcataria .…”

Section: Dna Extraction Ssr Primers and Pcr Amplificationmentioning

confidence: 99%

Development of simple sequence repeat (SSR) markers and genetic diversity analysis in blackwood (Acacia melanoxylon) clones in china

et al. 2016

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Understanding the genetic diversity of Acacia melanoxylon is very important in species selection and improvement. The present study aimed to identify microsatellite markers and determine the genetic diversity of 45 preferred clones selected from 9 Chinese districts. Seventy-six simple sequence repeat (SSR) markers developed for other Acacia species were screened. Seventeen SSR markers showed polymorphic patterns and amplified 134 alleles. Polymorphism information content (PIC) values ranged from 0.044 to 0.911. The average allele number per locus ranged between 2 and 18, averaging 8.06. Nine SSR markers were highly informative, with PIC values above 0.60. These findings demonstrated that SSR markers could be used to differentiate A. melanoxylon genotypes. Cluster analysis using UPGMA separated the 45 clones into 3 distinct groups at a similarity coefficient of 0.72. The clone groups identified in this study would be useful in developing intraspecific hybrids to exploit hybrid vigor as well as for commercial cultivation and genetic base broadening. The DNA fingerprints obtained for each clone could be used for biodiversity conservation. Keywords: Acacia melanoxylon, SSR, genetic diversity, polymorphism information contentDevelopment of simple sequence repeat (SSR) markers and genetic diversity analysis in blackwood (Acacia melanoxylon) clones in china

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A multiplexed set of microsatellite markers for discriminating Acacia mangium, A. auriculiformis, and their hybrid

Cited by 14 publications

References 47 publications

Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity

Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity

Observations on the Successes and Failures of Acacia Plantations in Sabah and Sarawak and the Way Forward

Development of simple sequence repeat (SSR) markers and genetic diversity analysis in blackwood (Acacia melanoxylon) clones in china

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