A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice

Byrne, Alana B.; García, Ayelén G.; Brahamian, Jorge M; Mauri, Aldana; Ferretti, Adrián; Polack, Fernando P.; Talarico, Laura

doi:10.1002/ame2.12145

Cited by 16 publications

(14 citation statements)

References 58 publications

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“…Almost all the parameters did not change significantly. Moreover, the levels of lactate dehydrogenase activity, red blood cell counts, hemoglobin concentration, mean corpuscular hemoglobin concentration, and hematocrit are in the normal BALB/c ranges. − The changes in white blood cell and monocyte counts are slight, and less than 15% exceed the higher limits of the normal levels . Thus, we concluded that the nanoparticles should not have any relevant side effects.…”

Section: Resultsmentioning

confidence: 98%

Green Synthesis of Size-Controlled in Vivo Biocompatible Immunoglobulin-Based Nanoparticles by a Swift Thermal Formation

Lunin

Korenkov

Mochalova

et al. 2021

ACS Sustainable Chem. Eng.

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Protein-based nanoparticles are promising candidates to be used as nanomedicine due to their high biocompatibility. On the other hand, functional nanoagents are fabricated using complex, nonscalable, and environmentally unfriendly procedures. An urge to meet multiple biomedical and industrial requirements highlights a need to find a universal, ecofriendly, and flexible synthetic strategy of functional nanoagent engineering. Here, we show a green, ultrafacile, and size-controlled synthesis (swift thermal formation; STF) of monodisperse immunoglobulin-based nanoparticles. The synthesis allows the fabrication of hybrid nanoparticles as well. We show that the nanoparticles retain the specificity of immunoglobulins and can target malignant cells. In addition, we demonstrate that the nanoparticles are biocompatible and applicable in vivo. Tunability, ease, and sustainability of the synthesis may turn the targeted nanoagent design into a routine procedure. This ready-to-use solution could assist a broad spectrum of biomedical applications, e.g., imaging and therapy.

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Section: Resultsmentioning

confidence: 98%

Green Synthesis of Size-Controlled in Vivo Biocompatible Immunoglobulin-Based Nanoparticles by a Swift Thermal Formation

Lunin

Korenkov

Mochalova

et al. 2021

ACS Sustainable Chem. Eng.

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“…We verified the role of vimentin in DENV invasion in SV129 suckling mice with and without vimentin knockout. Many models have been used to study DENV, including A/J ( Shresta et al., 2004 ), BALB/c ( Ahmad et al., 2019 ), C57BL/6 ( Byrne et al., 2020 ), and AG129 ( Tan et al., 2010 ) mice for dengue virus tropism and pathogenic research; the DENV-infected non-human primate ( Kayesh and Tsukiyama-Kohara, 2022 ), AG129 mouse continuous infection, cross-infection-established ADE mouse models to study the immune mechanisms of DENV infection ( Blaney et al., 2002 ); and DENV-infected suckling mouse ( Pelliccia et al., 2017 ) and DENV-infected SCID mouse models in vaccine research. In this study, we used suckling mice because adult mice displayed no obvious clinical signs after infection, and DENV-2 was not detected in the serum.…”

Section: Discussionmentioning

confidence: 99%

Vimentin Inhibits Dengue Virus Type 2 Invasion of the Blood-Brain Barrier

Zhou

et al. 2022

Front. Cell. Infect. Microbiol.

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Dengue virus (DENV) causes dengue fever, which is prevalent in the tropical and subtropical regions, and in recent years, has resulted in several major epidemics. Vimentin, a cytoskeletal component involved in DENV infection, is significantly reorganized during infection. However, the mechanism underlying the association between DENV infection and vimentin is still poorly understood. We generated vimentin-knockout (Vim-KO) human brain microvascular endothelial cells (HBMECs) and a Vim-KO SV129 suckling mouse model, combining the dynamic vimentin changes observed in vitro and differences in disease course in vivo, to clarify the role of vimentin in DENV-2 infection. We found that the phosphorylation and solubility of vimentin changed dynamically during DENV-2 infection of HBMECs, suggesting the regulation of vimentin by DENV-2 infection. The similar trends observed in the phosphorylation and solubility of vimentin showed that these characteristics are related. Compared with that in control cells, the DENV-2 viral load was significantly increased in Vim-KO HBMECs, and after DENV-2 infection, Vim-KO SV129 mice displayed more severe disease signs than wild-type SV129 mice, as well as higher viral loads in their serum and brain tissue, demonstrating that vimentin can inhibit DENV-2 infection. Moreover, Vim-KO SV129 mice had more disordered cerebral cortical nerve cells, confirming that Vim-KO mice were more susceptible to DENV-2 infection, which causes severe brain damage. The findings of our study help clarify the mechanism by which vimentin inhibits DENV-2 infection and provides guidance for antiviral treatment strategies for DENV infections.

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“…Blood samples were collected daily postinfection for viremia detection 6 . Viral RNA was extracted using the NucleoSpin® RNA Virus and quantified by quantitative reverse‐transcription PCR (qRT‐PCR) using an ABI StepOnePlus TM real‐time PCR system.…”

Section: Methodsmentioning

confidence: 99%

Murine diabetic models for dengue virus infection

Liu

et al. 2022

Journal of Medical Virology

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Dengue virus (DENV) is a critical public health concern in tropical and subtropical regions worldwide. Thus, immunocompetent murine models of DENV infection with robust viremia are required for vaccine studies. Diabetes is highly prevalent worldwide, making it frequent comorbidity in patients with dengue fever. Therefore, murine models are needed to understand viral pathogenesis and disease progression. Acquired‐induced and inherently diabetic C57BL/6 and db/db mice were inoculated with DENV‐3 via the tail vein. After infection, both the diabetic C57BL/6 and db/db mice showed obvious weight loss with clinical manifestations. Quantitative reverse‐transcription polymerase chain reaction revealed robust and replicable viremia in the two types of diabetic mice. Immunohistochemical detection showed persistent DENV‐3 infection in the liver. Enzyme‐linked immunosorbent assay for cytokine detection revealed that diabetic mice showed more severe inflammatory responses than did nondiabetic mice, and significant histological alterations were observed in diabetic mice. Thus, the diabetic mice were more susceptible to DENV infection than the nondiabetic mice. Taken together, we established two types of immunocompetent diabetic mice for DENV infection, which can be used to further study the mechanisms of dengue pathogenesis in diabetes and to develop antiviral pharmaceuticals and treatments.

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A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice

Cited by 16 publications

References 58 publications

Green Synthesis of Size-Controlled in Vivo Biocompatible Immunoglobulin-Based Nanoparticles by a Swift Thermal Formation

Green Synthesis of Size-Controlled in Vivo Biocompatible Immunoglobulin-Based Nanoparticles by a Swift Thermal Formation

Vimentin Inhibits Dengue Virus Type 2 Invasion of the Blood-Brain Barrier

Murine diabetic models for dengue virus infection

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