A muscle acetylcholine receptor is expressed in the human cerebellar medulloblastoma cell line TE671

Abstract

The human neuromedulloblastoma cell line TE671 is shown by single-channel recordings to express nicotinic acetylcholine receptors (AChRs) that are blocked by alpha-bungarotoxin (alpha Bgt). These AChRs do not react with antisera to the alpha Bgt-binding protein of brain or with monoclonal antibodies (mAbs) to brain nicotinic AChRs that do not bind alpha Bgt. TE671 AChRs do react with autoantibodies to muscle AChRs from myasthenia gravis patients and with mAbs to muscle AChRs, including mAbs specific for extrajunctional AChRs. AChRs. AChRs purified from TE671 cells are composed of 4 kinds of subunits corresponding to those of muscle AChR. Sequences of cDNAs for the ACh-binding alpha subunit and the delta subunit of this AChR further identify it as muscle AChR. Expression of TE671 AChR can be up-regulated by nicotine and dexamethasone, and down-regulated by forskolin.

Age- and sex-related resistance to chronic experimental autoimmune myasthenia gravis (EAMG) in Brown Norway rats

“…The rhabdomyosarcoma cell line TE671 expresses human nicotinic AChR [26]. TE671 cells were cultured to confluency at 378C in 24-well tissue culture plates (Costar, Europe Ltd, Badhoevedorp, The Netherlands) in Iscove's modified Dulbecco's medium (IMDM), supplemented with 5% fetal calf serum (FCS), 1% penicillin/streptomycin, 1% pyruvate and 2 .…”

Characterisation, crystallisation and preliminary X‐ray diffraction analysis of a Fab fragment of a rat monoclonal antibody with very high affinity for the human muscle acetylcholine receptor

“…Therefore, the reduced affinity of mAb192 for rat AChR should be due to differences within the c1 subunit and not to conformational constraints imposed by the other rat subunits. There are only two residues in the extracellular parts of the AChR CL subunit (residues ~~-209, 268-292 and 428437) in which the rat ~1 subunit differs from both the human and mouse a subunits [17,18]. These are residues 23 (Glu -+ Gly) and 195 (Asp, Thr+Asn).…”

A new method to induce myasthenia gravis models and the protective effect of soluble decay accelerating factors

“…AChRTE is able to react with the AChRMU mAbs, including mAbs specific to AChR of the ECD and MIR regions. It is possible to express AChRMU α subunit ECD protein using AChRTE recombination (Luther et al, 1989).…”

“…Literature on AChR expression in the human muscle is limited (Luther et al, 1989;Talib et al, 1991). Therefore, we used the AChRTEα1 subunit ECD fragment recombinant to express the ECD (α1-207) protein.…”