A mutation affecting curd colour in cauliflower (Brassica oleracea L. var. Botrytis DC)

Crisp, P.; Walkey, D. G. A.; Bellman, Elaine; Roberts, Elaine

doi:10.1007/bf00147182

Cited by 46 publications

(25 citation statements)

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“…A spontaneous, semidominant Orange (Or) mutant in cauliflower (Brassica oleracea var botrytis) represents an interesting genetic mutation that confers carotenoid accumulation in normally unpigmented tissues (Crisp et al, 1975;Dickson et al, 1988). It provides an excellent model to elucidate the regulatory control of carotenoid accumulation.…”

Section: Introductionmentioning

confidence: 99%

The CauliflowerOrGene Encodes a DnaJ Cysteine-Rich Domain-Containing Protein That Mediates High Levels of β-Carotene Accumulation

et al. 2006

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Despite recent progress in our understanding of carotenogenesis in plants, the mechanisms that govern overall carotenoid accumulation remain largely unknown. The Orange (Or) gene mutation in cauliflower (Brassica oleracea var botrytis) confers the accumulation of high levels of β-carotene in various tissues normally devoid of carotenoids. Using positional cloning, we isolated the gene representing Or and verified it by functional complementation in wild-type cauliflower. Or encodes a plastid-associated protein containing a DnaJ Cys-rich domain. The Or gene mutation is due to the insertion of a long terminal repeat retrotransposon in the Or allele. Or appears to be plant specific and is highly conserved among divergent plant species. Analyses of the gene, the gene product, and the cytological effects of the Or transgene suggest that the functional role of Or is associated with a cellular process that triggers the differentiation of proplastids or other noncolored plastids into chromoplasts for carotenoid accumulation. Moreover, we demonstrate that Or can be used as a novel genetic tool to induce carotenoid accumulation in a major staple food crop. We show here that controlling the formation of chromoplasts is an important mechanism by which carotenoid accumulation is regulated in plants.

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Section: Introductionmentioning

confidence: 99%

The CauliflowerOrGene Encodes a DnaJ Cysteine-Rich Domain-Containing Protein That Mediates High Levels of β-Carotene Accumulation

et al. 2006

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“…One of the factor is Orange (Or) which is firstly isolated in mutant of cauliflower, Brassica oleracea var. Botrytis (Crisp et al 1975;Li et al 2006;Lu et al 2006) and recent studies showed that Or is post-translational stabilizer of PSY (Park et al 2016;Zhou et al 2015). Overexpression of Or would cause potential of chromoplast differentiation and facilitate more accumulation of carotenoids.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of carotenogenic genes in the Japanese morning glory Ipomoea (Pharbitis) nil

Watanabe

Oda-Yamamizo

Sage-Ono

et al. 2017

Plant Biotechnology

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Japanese morning glory, Ipomoea nil, has several coloured flowers except yellow, because it can accumulate only trace amounts of carotenoids in the petal. To make the petal yellow with carotenoids, we introduced five carotenogenic genes (geranylgeranyl pyrophosphate synthase, phytoene synthase, lycopene β-cyclase and β-ring hydroxylase from Ipomoea obscura var. lutea and bacterial phytoene desaturase from Pantoea ananatis) to white-flowered I. nil cv. AK77 with a petal-specific promoter by Rhizobium (Agrobacterium)-mediated transformation method. We succeeded to produce transgenic plants overexpressing carotenogenic genes. In the petal of the transgenic plants, mRNA levels of the carotenogenic genes were 10 to 1,000 times higher than those of non-transgenic control. The petal colour did not change visually; however, carotenoid concentration in the petal was increased up to about ten-fold relative to non-transgenic control. Moreover, the components of carotenoids in the petal were diversified, in particular, several β-carotene derivatives, such as zeaxanthin and neoxanthin, were newly synthesized. This is the first report, to our knowledge, of changing the component and increasing the amount of carotenoid in petals that lack ability to biosynthesize carotenoids.

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“…One gene expresses white dominant to yellow . Another gene with partial dominance for white curds has been identified, with a double recessive causing dwarf, orange curds (Crisp et al ., 1975) . There is an independent codominant gene for greenless .…”

Section: Glucosinolatesmentioning

confidence: 99%

Molecular genetics and breeding of vegetable brassicas

King¹

1990

Euphytica

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These Brassicas are the most baffling plants' (Bailey, 1922) Summary Genetic traits relevant to breeding vegetable brassicas are surveyed to determine the effects that recent advances in molecular genetics and molecular marker technology might have on breeding priorities and practices. The problems peculiar to breeding horticultural crops are addressed . The application of DNA restriction fragment length polymorphisms (RFLPs) as an aid to breeding brassicas is discussed and genes amenable to molecular analysis are considered in the light of both experimental and economic factors .

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A mutation affecting curd colour in cauliflower (Brassica oleracea L. var. Botrytis DC)

Cited by 46 publications

References 1 publication

The CauliflowerOrGene Encodes a DnaJ Cysteine-Rich Domain-Containing Protein That Mediates High Levels of β-Carotene Accumulation

The CauliflowerOrGene Encodes a DnaJ Cysteine-Rich Domain-Containing Protein That Mediates High Levels of β-Carotene Accumulation

Overexpression of carotenogenic genes in the Japanese morning glory <i>Ipomoea</i> (<i>Pharbitis</i>) <i>nil</i>

Molecular genetics and breeding of vegetable brassicas

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