A mutation affecting <i>ASCORBATE PEROXIDASE 2</i> gene expression reveals a link between responses to high light and drought tolerance

Rossel, Jan Bart; Walter, Philippa B.; Hendrickson, Luke; Chow, Wah Soon; Poole, Andrew T.; Mullineaux, Philip M.; Pogson, Barry J.

doi:10.1111/j.1365-3040.2005.01419.x

Cited by 166 publications

(171 citation statements)

References 53 publications

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“…Genomic responses of A. thaliana to different types of environmental stress have often been studied independently of one another. There has been an increasing recognition, however, of the genetic and physiological elements that are shared Rossel et al 2006). The present study has examined associations among gene expression responses to nine types of abiotic stress, which represents the most inclusive analysis of stress-induced transcriptional changes currently available in the Arabidopsis model system.…”

Section: Discussionmentioning

confidence: 99%

The Association Among Gene Expression Responses to Nine Abiotic Stress Treatments in Arabidopsis thaliana

Swindell

2006

Genetics

114

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The identification and analysis of genes exhibiting large expression responses to several different types of stress may provide insights into the functional basis of multiple stress tolerance in plant species. This study considered whole-genome transcriptional profiles from Arabidopsis thaliana root and shoot organs under nine abiotic stress conditions (cold, osmotic stress, salt, drought, genotoxic stress, ultraviolet light, oxidative stress, wounding, and high temperature) and at six different time points of stress exposure (0.5, 1, 3, 6, 12, and 24 hr). In roots, genomewide correlations between transcriptional responses to different stress treatments peaked following 1 hr of stress exposure, while in shoots, correlations tended to increase following 6 hr of stress exposure. The generality of stress responses at the transcriptional level was therefore time and organ dependent. A total of 67 genes were identified as exhibiting a statistically significant pattern of gene expression characterized by large transcriptional responses to all nine stress treatments. Most genes were identified from early to middle (1-6 hr) time points of stress exposure. Analysis of this gene set indicated that cell rescue/defense/virulence, energy, and metabolism functional classes were overrepresented, providing novel insight into the functional basis of multiple stress tolerance in Arabidopsis.T HE genetic effects of environmental stress have been extensively studied from the standpoint of cellular physiology (Singh et al. 2002;Mahalingam et al.

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Section: Discussionmentioning

confidence: 99%

The Association Among Gene Expression Responses to Nine Abiotic Stress Treatments in Arabidopsis thaliana

Swindell

2006

Genetics

114

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“…Previously reported genetic screens failed to identify positive regulators of APX2 gene expression (15,16), suggesting that these regulators may be redundantly encoded in the Arabidopsis genome. In addition, the rapid kinetics of the response, its independence of new protein synthesis, and the preponderance of HSEs predicted in the promoters of up-regulated genes was reminiscent of the response to heat shock in many organisms.…”

Section: Heat-shock Transcription Elements Are Necessary and Sufficiementioning

confidence: 99%

“…Despite APX2's fast and very strong induction upon exposure to EL (11), genetic screens have identified only constitutive APX2 expression mutants, but no mutants affected specifically in EL-driven APX2 induction have been found (15,16). There is evidence linking APX2 induction by EL to the zinc-finger transcription factor ZAT10, to the plant hormone abscisic acid, and to 3′-phosphoadenosine 5′-phosphate, a phosphonucleotide (PAP) (5,17,18); however, a signal transduction pathway linking gene expression in the nucleus to redox of the PQ pool remains elusive.…”

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confidence: 99%

Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light

Jung

Crisp

Estavillo

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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136

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Sunlight provides energy for photosynthesis and is essential for nearly all life on earth. However, too much or too little light or rapidly fluctuating light conditions cause stress to plants. Rapid changes in the amount of light are perceived as a change in the reduced/oxidized (redox) state of photosynthetic electron transport components in chloroplasts. However, how this generates a signal that is relayed to changes in nuclear gene expression is not well understood. We modified redox state in the reference plant, Arabidopsis thaliana, using either excess light or low light plus the herbicide DBMIB (2,5-dibromo-3-methyl-6-isopropyl-pbenzoquinone), a well-known inhibitor of photosynthetic electron transport. Modification of redox state caused a change in expression of a common set of about 750 genes, many of which are known stress-responsive genes. Among the most highly enriched promoter elements in the induced gene set were heat-shock elements (HSEs), known motifs that change gene expression in response to high temperature in many systems. We show that HSEs from the promoter of the ASCORBATE PEROXIDASE 2 (APX2) gene were necessary and sufficient for APX2 expression in conditions of excess light, or under low light plus the herbicide. We tested APX2 expression phenotypes in overexpression and loss-of-function mutants of 15 Arabidopsis A-type heat-shock transcription factors (HSFs), and identified HSFA1D, HSFA2, and HSFA3 as key factors regulating APX2 expression in diverse stress conditions. Excess light regulates both the subcellular location of HSFA1D and its biochemical properties, making it a key early component of the excess light stress network of plants.

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“…The effect of ggt1-1 on ABA accumulation, along with observations that alterations in ROS metabolism can influence ABA metabolism (Pastori et al 2003;Rossel et al 2006); that ABA can regulate the expression of genes related to ROS metabolism (Jiang and Zhang 2002;Fryer et al 2003;Milla et al 2003); and that ROS status can influence stress-induced ABA accumulation (Hu et al 2005) indicate that there are likely to be numerous mechanisms by which ABA and ROS metabolism interact and cross regulate one another. At the cellular level, the recent report that the chloroplast-localized Mg-chelatase H subunit specifically binds ABA and is likely to be an ABA receptor (Shen et al 2006) supports the idea that H 2 O 2 produced in the chloroplast, one likely source of the increased H 2 O 2 production in ggt1-1, could affect ABA signaling.…”

Section: Increased Ros Levels Stimulate Aba-induced Pro Accumulationmentioning

confidence: 99%

Altered ABA, proline and hydrogen peroxide in an Arabidopsis glutamate:glyoxylate aminotransferase mutant

2007

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Plant responses to abiotic stress are determined both by the severity of the stress as well as the metabolic status of the plant. Abscisic acid (ABA) is a key component in integrating these various signals and controlling downstream stress responses. By screening for plants with decreased RD29A:LUC expression, we isolated two alleles, glutamate:glyoxylate transferase1-1 (ggt1-1) and ggt1-2, of a mutant with altered ABA sensitivity. In addition to reduced ABA induction of RD29A, ggt1-1 was altered in ABA and stress regulation of D 1 -pyrroline-5-carboxylate synthase, proline dehydrogenase and 9-cis-epoxycarotenoid dioxygenase 3, which encode enzymes involved in Pro and ABA metabolsim, respectively. ggt1-1 also had altered ABA and Pro contents after stress or ABA treatments while root growth and leaf water loss were relatively unaffected. A light-dependent increase in H 2 O 2 accumulation was observed in ggt1-1 consistent with the previously characterized role of GGT1 in photorespiration. Treatment with exogenous H 2 O 2 , as well as analysis of a mutant in nucleoside diphosphate kinase 2 which also had increased H 2 O 2 content but is not involved in photorespiration or amino acid metabolism, demonstrated that the greater ABA stimulation of Pro accumulation in these mutants was caused by altered H 2 O 2 content as opposed to other metabolic changes. The results suggest that metabolic changes that alter H 2 O 2 levels can affect both ABA accumulation and ABA sensitivity.

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A mutation affecting ASCORBATE PEROXIDASE 2 gene expression reveals a link between responses to high light and drought tolerance

Cited by 166 publications

References 53 publications

The Association Among Gene Expression Responses to Nine Abiotic Stress Treatments in Arabidopsis thaliana

The Association Among Gene Expression Responses to Nine Abiotic Stress Treatments in Arabidopsis thaliana

Subset of heat-shock transcription factors required for the early response of Arabidopsis to excess light

Altered ABA, proline and hydrogen peroxide in an Arabidopsis glutamate:glyoxylate aminotransferase mutant

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