A mutation in the enzyme monoamine oxidase explains part of the Astyanax cavefish behavioural syndrome

Elipot, Yannick; Hinaux, Hélène; Crinon, Jacques; Launay, Jean‐Marie; Blin, Maryline; Rétaux, Sylvie

doi:10.1038/ncomms4647

Cited by 80 publications

(69 citation statements)

References 61 publications

(89 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), including changes in rates of degradation of monoamines (Elipot et al . ). Behavioural data suggest that aggressiveness and sociability in sticklebacks and blind cavefish have evolved between natural populations (Strickler & Soares ; Elipot et al .…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Receptors rather than signals change in expression in four physiological regulatory networks during evolutionary divergence in threespine stickleback

et al. 2016

View full text Add to dashboard Cite

The molecular mechanisms underlying behavioural evolution following colonization of novel environments are largely unknown. Molecules that interact to control equilibrium within an organism form physiological regulatory networks. It is essential to determine whether particular components of physiological regulatory networks evolve or if the network as a whole is affected in populations diverging in behavioural responses, as this may affect the nature, amplitude and number of impacted traits. We studied the regulation of four physiological regulatory networks in freshwater and marine populations of threespine stickleback raised in a common environment, which were previously characterized as showing evolutionary divergence in behaviour and stress reactivity. We measured nineteen components of these networks (ligands and receptors) using mRNA and monoamine levels in the brain, pituitary and interrenal gland, as well as hormone levels. Freshwater fish showed higher expression in the brain of adrenergic (adrb2a), serotonergic (htr2a) and dopaminergic (DRD2) receptors, but lower expression of the htr2b receptor. Freshwater fish also showed higher expression of the mc2r receptor of the glucocorticoid axis in the interrenals. Collectively, our results suggest that the inheritance of the regulation of these networks may be implicated in the evolution of behaviour and stress reactivity in association with population divergence. Our results also suggest that evolutionary change in freshwater threespine stickleback may be more associated with the expression of specific receptors rather than with global changes of all the measured constituents of the physiological regulatory networks.

show abstract

Section: Discussionmentioning

confidence: 97%

“…A high ratio is indicative of elevated catabolic activity and has been interpreted as a high monoamine demand in the brain (Elipot et al . ; Loveland et al . ).…”

Section: Methodsmentioning

confidence: 98%

Receptors rather than signals change in expression in four physiological regulatory networks during evolutionary divergence in threespine stickleback

et al. 2016

View full text Add to dashboard Cite

show abstract

“…A well‐studied (and extreme) example of morphological change in response to environmental pressure is the Astyanax mexicanus (De Filippi, 1853) cavefish. Thousands of years ago, surface‐dwelling forms of A. mexicanus invaded caves through river systems, giving rise to a number of modified cave morphs well‐adapted to the hypogean habitat (Elipot et al, ; Gross, Borowsky, & Tabin, ; Jeffery, ; McGaugh et al, ; Protas, Conrad, Gross, Tabin, & Borowsky, ). Lacustrine (lake‐dwelling) species of the Astyanax genus have similarly evolved morphological changes relative to ecological distribution (Ornelas‐García, Bastir, & Doadrio, ; Ornelas‐García, Domínguez‐Domínguez, & Doadrio, ), including body size and shape changes (Garita‐Alvarado, Barluenga, & Ornelas‐García, ).…”

Section: Introductionmentioning

confidence: 99%

A geographical cline in craniofacial morphology across populations of Mesoamerican lake‐dwelling fishes

et al. 2020

View full text Add to dashboard Cite

Together, the complex geological history and climatic diversity of Mesoamerica create a rich source of biodiversity from which evolutionary processes can be studied. Here, we discuss highly divergent morphs of lake‐dwelling fishes distributed across Mexico and Central America, originally recognized as members of different genera (Astyanax and “Bramocharax”). Recent phylogenetic studies, however, suggest these morphs group within the same genus and readily hybridize. Despite genetic similarities, Bramocharax morphs exhibit stark differences in cranial shape and dentition. We investigated the evolution of several cranial traits that vary across morphs collected from four lakes in Mexico and Nicaragua and discovered an ecomorphological cline from northern to southern lakes. Northern populations of sympatric morphs exhibit a similar cranial shape and tooth morphology. Southern populations of Bramocharax morphs, however, showed a larger disparity in maxillary teeth, length and frequency of unicuspid teeth, an elongated snout, and a streamlined cranium compared to Astyanax morphs. This divergence of craniofacial morphology likely evolved in association with differences in trophic niches. We discuss the morphological differences across the four lake systems in terms of geological history and trophic dynamics. In summary, our study suggests that Bramocharax morphs are likely locally adapted members derived from independent Astyanax lineages, highlighting an interesting parallel evolutionary pattern within the Astyanax genus.

show abstract

“…The identification of these circuits allows for cell‐autonomous manipulation of gene function, or optogenetic manipulation of behaviorally relevant neurons. In A. mexicanus , several genes have been implicated in behavior including Mc4r in feeding, hypocretin in sleep, and monoaminergic signaling in aggression . While presumably the neurons expressing these genes are implicated in behavior, a lack of transgenic accessibility represents a fundamental impediment to mechanistic investigation.…”

Section: Discussionmentioning

confidence: 99%

Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system

Stahl

Peuß

McDole

et al. 2019

Developmental Dynamics

View full text Add to dashboard Cite

Background Astyanax mexicanus is a well‐established fish model system for evolutionary and developmental biology research. These fish exist as surface forms that inhabit rivers and 30 different populations of cavefish. Despite important progress in the deployment of new technologies, deep mechanistic insights into the genetic basis of evolution, development, and behavior have been limited by a lack of transgenic lines commonly used in genetic model systems. Results Here, we expand the toolkit of transgenesis by characterizing two novel stable transgenic lines that were generated using the highly efficient Tol2 system, commonly used to generate transgenic zebrafish. A stable transgenic line consisting of the zebrafish ubiquitin promoter expresses enhanced green fluorescent protein ubiquitously throughout development in a surface population of Astyanax. To define specific cell‐types, a Cntnap2‐mCherry construct labels lateral line mechanosensory neurons in zebrafish. Strikingly, both constructs appear to label the predicted cell types, suggesting many genetic tools and defined promoter regions in zebrafish are directly transferrable to cavefish. Conclusion The lines provide proof‐of‐principle for the application of Tol2 transgenic technology in A. mexicanus. Expansion on these initial transgenic lines will provide a platform to address broadly important problems in the quest to bridge the genotype‐phenotype gap.

show abstract

A mutation in the enzyme monoamine oxidase explains part of the Astyanax cavefish behavioural syndrome

Cited by 80 publications

References 61 publications

Receptors rather than signals change in expression in four physiological regulatory networks during evolutionary divergence in threespine stickleback

Receptors rather than signals change in expression in four physiological regulatory networks during evolutionary divergence in threespine stickleback

A geographical cline in craniofacial morphology across populations of Mesoamerican lake‐dwelling fishes

Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system

Contact Info

Product

Resources

About