A mutation in the melon Vacuolar Protein Sorting 41prevents systemic infection of Cucumber mosaic virus

Abstract

In the melon exotic accession PI 161375, the gene cmv1, confers recessive resistance to Cucumber mosaic virus (CMV) strains of subgroup II. cmv1 prevents the systemic infection by restricting the virus to the bundle sheath cells and impeding viral loading to the phloem. Here we report the fine mapping and cloning of cmv1. Screening of an F2 population reduced the cmv1 region to a 132 Kb interval that includes a Vacuolar Protein Sorting 41 gene. CmVPS41 is conserved among plants, animals and yeast and is required for post-Golgi vesicle trafficking towards the vacuole. We have validated CmVPS41 as the gene responsible for the resistance, both by generating CMV susceptible transgenic melon plants, expressing the susceptible allele in the resistant cultivar and by characterizing CmVPS41 TILLING mutants with reduced susceptibility to CMV. Finally, a core collection of 52 melon accessions allowed us to identify a single amino acid substitution (L348R) as the only polymorphism associated with the resistant phenotype. CmVPS41 is the first natural recessive resistance gene found to be involved in viral transport and its cellular function suggests that CMV might use CmVPS41 for its own transport towards the phloem.

A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease

“…6b). So far, only one other recessive gene in the plant kingdom, encoding a melon vacuolar protein sorting 41 mutant, has been reported to prevent systemic infection, in that case by Cucumber mosaic virus 17 . Comparatively, we here revealed a naturally occurring recessive gene ZmGDIα-hel that directly targets viral transport to confer passive resistance to MRDD.…”

“…The loci encoding these modified proteins generally act as recessive resistance genes. To date, many naturally occurring plant recessive resistance genes have been isolated and characterized, most of them related to the eukaryotic translation initiation factors eIF4E and eIF4G and their isoforms (eIFiso4E and eIFiso4G) 5,[10][11][12][13][14] , and some are mutants of other types of genes, such as HvPDIL5-1, cPGK2, and CmVPS41 [15][16][17] .…”

A Major QTL Located in Chromosome 8 of Cucurbita moschata Is Responsible for Resistance to Tomato Leaf Curl New Delhi Virus

“…In addition, other strategies have been reported for recessive resistance against viruses. The recessive cmv1 gene that confers resistance to cucumber mosaic virus in melon encodes a vacuolar protein sorting 41 (VPS41) (Giner et al, 2017) involved in membrane trafficking to the vacuole. Membrane components are key factors required for plant infection success, and viral replication is associated with host intracellular membranes (Nicaise, 2014).…”

Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9

“…A fundamental part of the genome editing process is the identification of target genes that determine phenotypes of interest, such as susceptibility to viruses (Garcia-Ruiz, 2018), other pathogens, resistance to herbicides or adverse environmental factors ( Table 1). Assessment of natural variation or systematic genome-wide screens are also powerful approaches to identify target genes (Kushner et al, 2003;Panavas et al, 2005;Pyott et al, 2016;Giner et al, 2017).…”