A mutually assured destruction mechanism attenuates light signaling in
            <i>Arabidopsis</i>

Ni, Wenjie; Xu, Shan-Mei; Tepperman, James M.; Stanley, David J.; Maltby, Dave A.; Gross, John D.; Burlingame, Alma L.; Wang, Wenfei; Quail, Peter H.

doi:10.1126/science.1250778

Cited by 236 publications

(299 citation statements)

References 27 publications

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“…7). These data suggest that, unlike PIF3 and phyB 23 , PIF1 and phyA may not be co-degraded through the CUL4 COP1-SPA E3 ubiquitin ligase complexes.…”

Section: Cop1mentioning

confidence: 89%

“…Plates were wrapped in aluminium foil at 21°C for 6 days in the dark before being The relative abundance of PIF1 and PIF3 levels in cop1 and spa mutants also highlights the differences in the mechanisms of PIF regulation. PIF3 has been shown to be unstable in the cop1-4 and spa123 triple-mutant backgrounds in the dark 23,24,31 , suggesting that COP1 and SPA proteins are necessary for the stability of PIF3 in the dark. In contrast, while PIF1 level is also lower in cop1-4 compared with wild type, PIF1 is much more abundant in the spaQ mutants compared with wild type in the dark (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a CUL3 LRB E3 ubiquitin ligase has been shown to be necessary for the light-induced co-degradation of PIF3 and phyB 23 . To examine whether PIF1 is also degraded through other Cullins, we performed immunoblots for PIF1 levels in cul1 and cul3 mutants.…”

Section: Cop1mentioning

confidence: 99%

“…The light-induced degradation of PIFs is UPS-mediated and involves phosphorylation and polyubiquitylation of PIFs 16,17,20,23 . We examined whether the phosphorylation or ubiquitylation of PIF1 is affected in the cop1-4, spaQ and cul4cs seedlings.…”

Section: Cop1mentioning

confidence: 99%

“…However, both CK2-and BIN2-mediated phosphorylation of PIFs are light independent, suggesting that additional kinases are necessary for light-induced phosphorylation of PIFs 22 . Recently, a CUL3 LRB E3 ubiquitin ligase was found to be necessary for the light-induced ubiquitylation and subsequent co-degradation of PIF3 and phyB under light 23 . However, the kinase and the E3 ubiquitin ligase necessary for the light-induced degradation of PIF1 are still unknown.…”

mentioning

confidence: 99%

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CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1

et al. 2015

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Plants undergo contrasting developmental programs in dark and light. Photomorphogenesis, a light-adapted programme is repressed in the dark by the synergistic actions of CUL4 COP1-SPA E3 ubiquitin ligase and a subset of basic helix-loop-helix transcription factors called phytochrome interacting factors (PIFs). To promote photomorphogenesis, light activates the phytochrome family of sensory photoreceptors, which inhibits these repressors by poorly understood mechanisms. Here, we show that the CUL4 COP1-SPA E3 ubiquitin ligase is necessary for the light-induced degradation of PIF1 in Arabidopsis. The light-induced ubiquitylation and subsequent degradation of PIF1 is reduced in the cop1, spaQ and cul4 backgrounds. COP1, SPA1 and CUL4 preferentially form complexes with the phosphorylated forms of PIF1 in response to light. The cop1 and spaQ seeds display strong hyposensitive response to far-red light-mediated seed germination and light-regulated gene expression. These data show a mechanism by which an E3 ligase attenuates its activity by degrading its cofactor in response to light.

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“…7). These data suggest that, unlike PIF3 and phyB 23 , PIF1 and phyA may not be co-degraded through the CUL4 COP1-SPA E3 ubiquitin ligase complexes.…”

Section: Cop1mentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Cop1mentioning

confidence: 99%

Section: Cop1mentioning

confidence: 99%

mentioning

confidence: 99%

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CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1

et al. 2015

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The Role of Phytochromes in Triggering Plant Developmental Transitions

Kaiserli

Chory

2016

Encyclopedia of Life Sciences

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Light is an essential stimulus for energy production, plant growth, development and adaptation to constantly changing environmental conditions. Plants sense different wavelengths of light through the action of specialised families of photoreceptor proteins. The molecular and physiological role of the phytochrome family of red/far‐red light receptors is well characterised. Upon light activation, phytochromes trigger multicomponent signalling cascades to induce fundamental cellular processes ranging from gene expression to protein abundance and nuclear architecture to regulate various aspects of plant development. Phytochrome responses are cell autonomous but in some cases mediate interorgan communication to control major developmental and adaptive responses such as flowering initiation and shade avoidance. Reciprocal interactions and integration between phytochrome and circadian signalling pathways are essential for optimising growth and reproductive success during the life cycle of the model plant Arabidopsis thaliana . Key Concepts Light is a vital informational signal for plant survival and growth. The phytochrome family of plant photoreceptors acts as molecular photoswitches in response to red and far‐red light. Plant phytochrome signal transduction regulates molecular and cellular processes. Phytochromes induce cell‐autonomous responses and interorgan communication. Phytochromes regulate light‐induced developmental transitions as well as adaptation to growth under dense canopy. Plant phytochromes have antagonistic and synergistic roles in regulating photoperiodic flowering in Arabidopsis . Phytochromes inform the endogenous clock about seasonal and daily changes to optimise plant growth and establish reproductive success.

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Nucleus‐to‐Plastid Phytochrome Signalling in Controlling Chloroplast Biogenesis

Yoo

Han

Chen

2020

Annual Plant Reviews Online

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The biogenesis of photosynthetically active chloroplasts in flowering plants (angiosperms) is initiated by light through the red and far‐red photoreceptors, phytochromes, which activate photosynthesis‐associated genes encoded by both the nuclear and plastid genomes. Because photoactivated phytochromes localize to the nucleus but not the plastids, phytochromes ought to control plastid transcription through nucleus‐to‐plastid or anterograde signalling. However, the mechanism of anterograde signalling remains poorly understood. Recent advances in our understanding of phytochrome signalling and plastid transcription led to the discovery of the framework of an anterograde signalling pathway, which links light‐dependent regulation of nuclear repressors of chloroplast biogenesis to the control of the assembly and activity of the bacterial‐type plastidial RNA polymerase for transcribing plastid‐encoded photosynthesis genes. This article is aimed to gather and reanalyze the evidence in the literature relevant to the phytochrome control of chloroplast biogenesis to forge a model of anterograde signalling mechanisms for the regulation of plastid transcription by light.

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A mutually assured destruction mechanism attenuates light signaling in Arabidopsis

Cited by 236 publications

References 27 publications

CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1

CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1

The Role of Phytochromes in Triggering Plant Developmental Transitions

Nucleus‐to‐Plastid Phytochrome Signalling in Controlling Chloroplast Biogenesis

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