A Mycoplasma strain F38 growth-inhibiting monoclonal antibody (WM-25) identifies an epitope on a surface-exposed polysaccharide antigen

Abstract

Monoclonal antibody (MAb) WM-25 differentiates by in vitro growth inhibition Mycoplasma capricolum subsp. capripneumoniae (Mycoplasma strain F38), which causes contagious caprine pleuropneumonia, from other Mycoplasma spp. (F. R. Rurangirwa, T. C. McGuire, A. J. Musoke, and A. Kibor, Infect. Immun. 55:3219-3220, 1987). The antigen identified by MAb WM-25 was isolated from solubilized Mycoplasma strain F38 organisms by MAb WM-25 affinity chromatography and was stained with Schiff's reagent, but not with Coomassie blue, after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Treatment of purified F38 polysaccharide with periodate abolished binding with MAb WM-25, and MAb WM-25 binding was blocked with laminarin, a complex oligosaccharide with ␤(133) sugar linkages. Purified F38 polysaccharide blocked both growth inhibition and agglutination of live F38 organisms caused by MAb WM-25 and rabbit antiserum to F38 organisms. The results in this paper demonstrate that MAb WM-25 binds a periodatesensitive epitope on the F38 polysaccharide which is also exposed on the surface of Mycoplasma strain F38. Because MAb WM-25 also causes in vitro growth inhibition of F38, the reactive polysaccharide epitope may induce protective immune responses.

Rapid Detection of Contagious Caprine Pleuropneumonia Using a Mycoplasma capricolum subsp. capripneumoniae Capsular Polysaccharide-Specific Antigen Detection Latex Agglutination Test

“…Detection of M. capricolum subsp. capripneumoniae antigen in the sera of infected goats (as reported in this paper and by others [23]) suggests either a hitherto undetected systemic phase to the infection or, perhaps more likely, that soluble antigen (CPS) is entering the circulation from the infected lung. Using both PCR and direct culture we were unable to detect M. capricolum subsp.…”

“…No agglutination was noted for any other mycoplasmal species tested, even in undiluted growth medium. The specificity of the LAT exactly mirrors that of the CPSspecific monoclonal antibody WM-25 (2,23), providing further evidence that CPS is the M. capricolum subsp. capripneumoniae antigen recognized by the LAT.…”

“…However, an exact correlation is unlikely since CFT detects a serological response, in contrast to LAT, which detects M. capricolum subsp. capripneumoniae antigen and which is therefore likely to be more sensitive at the early stages of infection (CPS antigen has been detected in the serum of experimentally infected animals within 4 to 9 days [23]) or 8 to 11 days [this study], while significant complement fixation titers were not observed until 21 days postinfection [17]).…”

“…capripneumoniae antigen should allow the diagnosis of a current infection, since antigen has been detected within 3 to 9 days of the onset of clinical signs following infection with M. capricolum subsp. capripneumoniae (23). Used in conjunction with the current antibody-detection LAT, this should allow effective field screening at all stages of infection.…”

Characterization of Strains of Mycoplasma mycoides subsp. mycoides Small Colony Type Isolated from Recent Outbreaks of Contagious Bovine Pleuropneumonia in Botswana and Tanzania: Evidence for a New Biotype

“…While cross protection using antisera raised against these two Mycoides cluster subspecies has been known for some time (due to the highly similar or identical CPS shared between M. capricolum subsp. capripneumoniae and bovine serogroup 7 [2,32]), we believe that this is the first time that antiserum raised against MmmSC has demonstrated GI activity against M. capricolum subsp. capripneumoniae.…”