A NAC transcription factor, NOR-like1, is a new positive regulator of tomato fruit ripening

Gao, Ying; Wei, Wei; Zhao, Xiaodan; Tan, Xiaoxiao; Fan, Zhengqiu; Zhang, Yiping; Yuan, Junfa; Meng, Lanhuan; Zhu, Beiwei; Zhu, Hongliang; Chen, Jianye; Jiang, Cai‐Zhong; Grierson, Donald; Luo, Yunbo; Fu, Daqi

doi:10.1038/s41438-018-0111-5

Cited by 183 publications

(183 citation statements)

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“…Evidence from other recent work (Gao et al , ) has confirmed that other TFs, including NACs, also modulate expression of genes involved in cell wall metabolism. There is some evidence that RIN interacts with a NAC protein, NAC4 (Zhu et al , ), and may also form heterodimers with other MADS TFs, such as TAGL1, AGL11 and FUL1/2 (reviewed in Li et al , ).…”

Section: Discussionmentioning

confidence: 71%

“…RIN expression is enhanced by ethylene (Giovannoni et al , ; Li et al , ; Lü et al , ). Other factors such as NOR, NOR‐like1, ERFs, ARFs and TAGL1, not mentioned in this model, also are involved in the ripening genetic programme (Li et al , ; Gao et al , ). (b) A model of the role of RIN and ET in regulating tomato fruit cell wall changes and softening.…”

Section: Discussionmentioning

confidence: 99%

“…RIN affects accumulation of many gene transcripts (Giovannoni, 2004), proteins and their post-translational degradation (Qin et al, 2012;Wang et al, 2014). Recent advances in knowledge concerning the transcription factors that regulate climacteric fruits ripening (Ito et al, 2017;Gao et al, 2018;Li et al, 2018;Wang et al, 2019), including discrepancies between the phenotypes of natural mutants and CRISPR/Cas9-induced mutations of ripening inhibitor (rin), non-ripening (nor) and colourless non-ripening (Cnr), have called into question the precise roles of these regulatory genes in the ripening control network (Ito et al, 2017;Gao et al, 2018;Li et al, 2019;Wang et al, 2019). The rin mutation was originally thought to correspond to a loss-of-function event (Vrebalov et al, 2002) but is now known to generate an active hybrid transcription factor, RIN-MC, which has a repressor activity (Ito et al, 2017;Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Roles of RIN and ethylene in tomato fruit ripening and ripening‐associated traits

Zhu

Pirrello³

et al. 2019

New Phytologist

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Summary RIPENING INHIBITOR (RIN)‐deficient fruits generated by CRISPR/Cas9 initiated partial ripening at a similar time to wild‐type (WT) fruits but only 10% WT concentrations of carotenoids and ethylene (ET) were synthesized. RIN‐deficient fruit never ripened completely, even when supplied with exogenous ET. The low amount of endogenous ET that they did produce was sufficient to enable ripening initiation and this could be suppressed by the ET perception inhibitor 1‐MCP. The reduced ET production by RIN‐deficient tomatoes was due to an inability to induce autocatalytic system‐2 ET synthesis, a characteristic feature of climacteric ripening. Production of volatiles and transcripts of key volatile biosynthetic genes also were greatly reduced in the absence of RIN. By contrast, the initial extent and rates of softening in the absence of RIN were similar to WT fruits, although detailed analysis showed that the expression of some cell wall‐modifying enzymes was delayed and others increased in the absence of RIN. These results support a model where RIN and ET, via ERFs, are required for full expression of ripening genes. Ethylene initiates ripening of mature green fruit, upregulates RIN expression and other changes, including system‐2 ET production. RIN, ET and other factors are required for completion of the full fruit‐ripening programme.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Roles of RIN and ethylene in tomato fruit ripening and ripening‐associated traits

Zhu

Pirrello³

et al. 2019

New Phytologist

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140

137

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“…In light of these studies, we speculate that the 485 action of the NAC18.1 transgenes was likely attenuated by the dominant-negative activity of the 486 spontaneous nor allele used in our work. Further heterologous characterization of NAC18.1 487 would likely benefit from the use of null nor mutants, or double mutants of nor and nor-like 1 488 (Gao et al, 2018). This may allow for more precise quantitative comparisons between alleles in 489 order to resolve whether differences in the coding sequence of NAC18.1 confer different degrees 490 of ripening.…”

Section: Apple 442mentioning

confidence: 99%

Apple ripening is controlled by a NAC transcription factor

Migicovsky

Yeats

Watts

et al. 2019

Preprint

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22Highlight: 23 NAC18.1 is a member of a family of conserved transcriptional regulators of ripening that 24 underlies variation in fruit firmness and harvest date in diverse apple accessions. 25 26 Abstract: 27Softening is a hallmark of ripening in fleshy fruits, and has both desirable and 28 undesirable implications for texture and postharvest stability. Accordingly, the timing and extent 29 of ripening and associated textural changes are key targets for improving fruit quality through 30 breeding. Previously, we identified a large effect locus associated with harvest date and firmness 31 in apple (Malus domestica) using genome-wide association studies (GWAS). Here, we present 32 additional evidence that polymorphisms in or around a transcription factor gene, NAC18.1, cause 33 variation in these traits. First, we confirmed our previous findings with new phenotype and 34 genotype data from ~800 apple accessions. In this population, we compared NAC18.1 to three 35 other ripening-related markers currently used by breeders (ACS1, ACO1, and PG1), and found 36 that the effect of the NAC18.1 genotype on both traits greatly exceeded that observed for the 37 other markers. By sequencing NAC18.1 across 18 accessions, we revealed two predominant 38 haplotypes containing the SNP previously identified using GWAS, as well as dozens of 39 additional SNPs and indels in both the coding and promoter sequences. NAC18.1 encodes a 40 protein with high similarity to the NON-RIPENING (NOR) transcription factor, an early 41 regulator of ripening in tomato (Solanum lycopersicum). To test whether these genes are 42 functionally orthologous, we introduced NAC18.1 transgenes into the tomato nor mutant and 43 showed that both haplotypes complement the nor ripening deficiency. Taken together, these 443 results indicate that polymorphisms in NAC18.1 underlie substantial variation in apple firmness 45 and harvest time through modulation of a conserved ripening program. 46 47

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“…With respect to the NAC TF family, functional characterization studies revealed that the NAC members participate in monitoring a wide spectrum of biological processes. Specifically, they have been shown to regulate embryo development [20,21], root development [22,23], shoot development [24], secondary cell wall formation [25,26], reproductive organ development [27,28], fiber development (e.g., in cotton) [29], trichome development [30,31], ripening [32] and senescence [33]. Their regulatory mechanisms have also been demonstrated to be coordinated by various hormone signaling pathways, including abscisic acid (ABA)- [34], jasmonic acid- [35,36], auxin- [37,38], ethylene- [39,40] and gibberellic acid-dependent [41] signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance in Arabidopsis in an Abscisic Acid-Dependent Manner

Hoang

Nguyen

et al. 2019

IJMS

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Being master regulators of gene expression, transcription factors (TFs) play important roles in determining plant growth, development and reproduction. To date, many TFs have been shown to positively mediate plant responses to environmental stresses. In the current study, the biological functions of a stress-responsive NAC [NAM (No Apical Meristem), ATAF1/2 (Arabidopsis Transcription Activation Factor1/2), CUC2 (Cup-shaped Cotyledon2)]-TF encoding gene isolated from soybean (GmNAC019) in relation to plant drought tolerance and abscisic acid (ABA) responses were investigated. By using a heterologous transgenic system, we revealed that transgenic Arabidopsis plants constitutively expressing the GmNAC019 gene exhibited higher survival rates in a soil-drying assay, which was associated with lower water loss rate in detached leaves, lower cellular hydrogen peroxide content and stronger antioxidant defense under water-stressed conditions. Additionally, the exogenous treatment of transgenic plants with ABA showed their hypersensitivity to this phytohormone, exhibiting lower rates of seed germination and green cotyledons. Taken together, these findings demonstrated that GmNAC019 functions as a positive regulator of ABA-mediated plant response to drought, and thus, it has potential utility for improving plant tolerance through molecular biotechnology.

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A NAC transcription factor, NOR-like1, is a new positive regulator of tomato fruit ripening

Cited by 183 publications

References 77 publications

Roles of RIN and ethylene in tomato fruit ripening and ripening‐associated traits

Roles of RIN and ethylene in tomato fruit ripening and ripening‐associated traits

Apple ripening is controlled by a NAC transcription factor

The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance in Arabidopsis in an Abscisic Acid-Dependent Manner

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