A nano-catalyst promoting endogenous NO production to enhance chemotherapy efficacy by vascular normalization

Wang, Xiaohui; Feng, Xiaoyue; Sun, Mengjie; Wang, Mingzhu; Wang, Wei; Yuan, Zhi

doi:10.1039/d2qm00133k

Cited by 4 publications

(3 citation statements)

References 44 publications

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“…We also assessed the pericyte coverage by co-staining the tumor tissue sections with the vessel marker CD31 and pericyte marker α-SMA. As shown in Figure A, the red fluorescence observed in the Cu 2 O@Dex group suggested that pericytes were properly attached to endothelial cells after treatment . Meanwhile, the semi-quantification results demonstrated that the pericyte coverage (α-SMA/CD31) increased to 33.9% after the Cu 2 O@Dex treatment (Figure B).…”

Section: Resultsmentioning

confidence: 83%

“…As shown in Figure 5A, the red fluorescence observed in the Cu 2 O@Dex group suggested that pericytes were properly attached to endothelial cells after treatment. 21 Meanwhile, the semi-quantification results demonstrated that the pericyte coverage (α-SMA/CD31) increased to 33.9% after the Cu 2 O@ Dex treatment (Figure 5B). The improvement of vessel normalization after Cu 2 O@Dex treatment could be attributed to the sustainable generation of the NO catalyst by the released Cu + .…”

Section: Vasculature Normalization Ability Of Cu 2 O@dex Nps In Vivomentioning

confidence: 93%

“…The NO generation driven by Cu + -catalyzed degradation of GSNO was investigated by the classical Griess method as in our earlier work. 21 To study how the pH value affects the catalytic reaction, the same concentration of Cu (5 μg mL −1 ) was added into the PBS solution of different pH values (pH 7.4, 6.5, or 5.5).…”

Section: Evaluation Of Hydroxyl Radical (•Oh)mentioning

confidence: 99%

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Cuprous Oxide-Based Dual Catalytic Nanostructures for Tumor Vascular Normalization-Enhanced Chemodynamic Therapy

Wang

Yan

Sun

et al. 2023

ACS Appl. Nano Mater.

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The abnormal blood vessels in tumor sites, causing poor tumor perfusion and hypoxia environment, restrict the chemodynamic therapy (CDT) potency significantly. To attain the synergistic therapy of tumor vascular normalization with CDT, herein, we constructed a dual-functional nanocatalyst with a simple structure, cuprous oxide by dextran (Cu 2 O@Dex), to restore proper tumor perfusion and tumor oxygenation. The in vitro experiment outcomes illustrated that Cu 2 O@Dex hardly releases Cu (Cu +/2+ ) under neutral conditions, which avoids unnecessary catalytic reaction in blood circulation. Under the weak acidic condition (pH 6.5), Cu 2 O@Dex begins to release Cu (Cu +/2+ ), catalyzing the nitric oxide (NO) generation for vascular normalization. Also, Cu 2 O@Dex further releases a large amount of Cu (Cu +/2+ ) under more acidic intratumor cells (pH 5.5), fast catalyzing the hydroxyl radical (•OH) generation for CDT. The in vivo experiment observations verified that Cu 2 O@Dex could improve tumor perfusion significantly 3 h post injection, which lasted for more than 144 h. The effective perfusion (Hoechst 33342 + ) area in the Cu 2 O@Dex group was 3.8-fold higher than that in the phosphate buffer saline (PBS) group after 48 h of injection, confirming the effectiveness of Cu 2 O@Dex to induce vascular normalization. Noticeably, tumor vascular normalization further enhanced the therapeutic effect of CDT through delivery of H 2 O 2 generator juglone (JUG) more smoothly. The antitumor efficacy of Cu 2 O@Dex plus JUG was 86.5% with no obvious toxicity. Overall, our design achieved the dual-catalytic effects integrated in one simple nanostructure for tumor vascular normalization-enhanced CDT.