A natural inhibitor from Alisma orientale against human carboxylesterase 2: Kinetics, circular dichroism spectroscopic analysis, and docking simulation

Jin, Yi; Bai, Rong; An, Yue; Liu, Tiantian; Liang, Jiahao; Tian, Xiangge; Huo, Xiaokui; Feng, Lei; Ning, Jing; Sun, Cheng‐Peng; Ma, Xiaochi; Zhang, Hou-Li

doi:10.1016/j.ijbiomac.2019.04.099

Cited by 24 publications

(13 citation statements)

References 32 publications

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“…In recent years, more and more attention has been paid to CES2, as it plays an important role in endogenous and exogenous metabolism (Di, 2019;Frank et al, 1986;Song et al, 2019;Yi et al, 2019). Due to the defects of in vitro and in vivo models, the study of CES2 is greatly limited.…”

Section: Discussionmentioning

confidence: 99%

Construction and Characterization of CRISPR/Cas9 Knockout Rat Model of Carboxylesterase 2a Gene

et al. 2021

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Carboxylesterase 2 (CES2), an important metabolic enzyme, plays a critical role in drug biotransformation and lipid metabolism. Although CES2 is very important, few animal models have been generated to study its properties and functions. Rat Ces2 is similar to human CES2A-CES3A-CES4A gene cluster, with highly similar gene structure, function and substrate. In this report, CRISPR/Cas9 technology was firstly used to knock out rat Ces2a, a main subtype of Ces2 mostly distributed in liver and intestine. This model showed the absence of CES2A protein expression in liver.Further pharmacokinetic studies of diltiazem, a typical substrate of CES2A, confirmed the loss of function of CES2A both in vivo and in vitro. At the same time, the expression of CES2C and CES2J protein in liver decreased significantly. The body and liver weight of Ces2a knockout rats also increased, but the food intake did not change. Moreover, the deficiency of Ces2a led to obesity, insulin resistance and liver fat accumulation, which are consistent with the symptoms of nonalcoholic fatty liver disease (NAFLD). Therefore, this rat model is not only a powerful tool to study drug metabolism mediated by CES2, but also a good disease model to study NAFLD.

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Section: Discussionmentioning

confidence: 99%

Construction and Characterization of CRISPR/Cas9 Knockout Rat Model of Carboxylesterase 2a Gene

et al. 2021

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“…The relative activities of OM-PLA1 (%) were calculated by dividing the enzyme activities of different factors with the control multiplied by 100% (Jiang et al, 2015). A concentration of 1 mM Mg 2+ affecting the secondary structure of OM-PLA1 was investigated by circular dichroism (CD) spectroscopy (Koubaa et al, 2019; Yi et al, 2019). The CD spectra of OM-PLA1 were obtained by Chirascan qCD (Applied Photophysics Ltd., UK).…”

Section: Methodsmentioning

confidence: 99%

“…The data were recorded from 190 to 260 nm with a time-per-point of 1 s and an interval of 1 nm (Koubaa et al, 2019). CDPro software was used to analyze the data (Yi et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Effective Expression of the Serratia marcescens Phospholipase A1 Gene in Escherichia coli BL21(DE3), Enzyme Characterization, and Crude Rapeseed Oil Degumming via a Free Enzyme Approach

Yang

Jiang

et al. 2019

Front. Bioeng. Biotechnol.

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Crude oil degumming by phospholipid removal is crucial to guarantee oil quality. Phospholipase degumming could produce green vegetable oil by reducing energy consumption and protecting the environment. To develop a novel phospholipase for oil degumming, we cloned the Serratia marcescens outer membrane phospholipase A gene (OM-PLA1) and expressed its 33 KDa protein in engineered Escherichia coli BL21(DE3). OM-PLA1 activity reached 18.9 U mL−1 with the induction of 0.6 mM isopropyl β-D-1-thiogalactopyranoside for 4 h. The optimum temperature and pH were 50°C and 7.5, respectively. Mg2+, Ca2+, Co2+, and Mn2+ at 0.1 mM L−1 significantly increased OM-PLA1 activity. The kinetic equations of OM-PLA1 and Lecitase Ultra were y = 13.7x+0.74 (Km = 18.53 mM, Vmax = 1.35 mM min−1) and y = 24.42x+0.58 (Km = 42.1 mM, Vmax = 1.72 mM min−1), respectively. The phosphorus content decreased from 22.6 to 9.3 mg kg−1 with the addition of 15 units of free recombinant OM-PLA1 into 150 g of crude rapeseed oil. OM-PLA1 has the close degumming efficiency with Lecitase Ultra. The S. marcescens outer membrane phospholipase gene (OM-PLA1) possessed higher substrate affinity and catalytic efficiency than Lecitase Ultra. This study provides an alternative approach to achieve crude vegetable oil degumming with enzymatic technology.

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“…(Li, Liu, Dai, Chen, & Li, 2019). In 2019, our group obtained 15 new protostanes from A. orientale , and their structures were defined as alismanins D‐H ( 61 and 65–68 ), 11‐deoxy alisol F 24‐acetate ( 62 ), 11‐deoxy‐12 β ‐hydroxy alisol F 24‐acetate ( 63 ), 11‐deoxy‐12 β ‐hydroxy‐16 β ‐methoxy‐16,23‐ seco alisol F 24‐acetate ( 64 ), 25‐ethoxy‐16‐oxo‐ 11‐anhydro alisol A ( 69 ), 11 β ‐methoxy alisol F ( 70 ), 25‐methoxy alisol F 24‐acetate ( 71 ), 11‐deoxy alisol F ( 72 ), 16‐oxo‐11‐anhydro alisol A 23,24‐acetonide ( 73 ), 11‐deoxy‐12 β ‐hydroxy alisol F ( 74 ), and alismanin I ( 75 ) (Luan et al, 2019; Yi et al, 2019).…”

Section: Phytochemical Constituents Of the Genus Alismamentioning

confidence: 99%

Alisma genus: Phytochemical constituents, biosynthesis, and biological activities

Feng

Liu

Huo

et al. 2020

Phytotherapy Research

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The genus Alisma contains 11 species distributed worldwide, of which at least two species (A. orientale [Sam.] Juzep. and A. plantago‐aquatica Linn.) have been used as common herbal medicines. Secondary metabolites obtained from the genus Alisma are considered to be the material basis for the various biological functions and medicinal applications. In this review, we mainly focused on the recent investigations of secondary metabolites from plants of the genus Alisma and their biological activities, with the highlighting on the diversity of the chemical structures, the biosynthesis of interesting secondary metabolites, the biological activities, and the relationships between structures and bioactivities.

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A natural inhibitor from Alisma orientale against human carboxylesterase 2: Kinetics, circular dichroism spectroscopic analysis, and docking simulation

Cited by 24 publications

References 32 publications

Construction and Characterization of CRISPR/Cas9 Knockout Rat Model of Carboxylesterase 2a Gene

Construction and Characterization of CRISPR/Cas9 Knockout Rat Model of Carboxylesterase 2a Gene

Effective Expression of the Serratia marcescens Phospholipase A1 Gene in Escherichia coli BL21(DE3), Enzyme Characterization, and Crude Rapeseed Oil Degumming via a Free Enzyme Approach

Alisma genus: Phytochemical constituents, biosynthesis, and biological activities

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