A Nd:YAG laser-microperforated poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-basal membrane matrix composite film as substrate for keratinocytes

Serrano, Fernando E.; López-G, Laura; Jadraque, María; Koper, Mariëlle; Ellis, Gary; Cano, Pilar; Martı́n, Margarita; Garrido, Leoncio

doi:10.1016/j.biomaterials.2006.09.018

Cited by 16 publications

(16 citation statements)

References 45 publications

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“…Biocompatibility is one of basic requirements for a qualified biomaterial. 17 Chitosan is nontoxic, antibacterial, 19 This study demonstrated that chitosan nanofibers did not change the morphologies or viabilities of mouse and human osteoblasts. Hence, chitosan nanofibers are biodegradable and nontoxic to osteoblasts.…”

Section: Discussionmentioning

confidence: 81%

“…To fulfill the functions of a biomaterial for bone tissue, the material should meet certain requirements of absorption kinetics, and have interconnected micropores and optimal porosity. 17 However, one major reason for orthopedic implant failure is the lack of sufficient integration of the implanted material into the juxtaposed bone. 18 Chitosan nanofibers possess a high surface area and porosity, and their use for fabricating biocompatible and biomimetic nanostructure scaffolds in tissue engineering has been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation

Liao²,

Lin³

et al. 2014

IJN

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Osteoblast maturation plays a key role in regulating osteogenesis. Electrospun nanofibrous products were reported to possess a high surface area and porosity. In this study, we developed chitosan nanofibers and examined the effects of nanofibrous scaffolds on osteoblast maturation and the possible mechanisms. Macro- and micro observations of the chitosan nanofibers revealed that these nanoproducts had a flat surface and well-distributed fibers with nanoscale diameters. Mouse osteoblasts were able to attach onto the chitosan nanofiber scaffolds, and the scaffolds degraded in a time-dependent manner. Analysis by scanning electron microscopy further showed mouse osteoblasts adhered onto the scaffolds along the nanofibers, and cell–cell communication was also detected. Mouse osteoblasts grew much better on chitosan nanofiber scaffolds than on chitosan films. In addition, human osteoblasts were able to adhere and grow on the chitosan nanofiber scaffolds. Interestingly, culturing human osteoblasts on chitosan nanofiber scaffolds time-dependently increased DNA replication and cell proliferation. In parallel, administration of human osteoblasts onto chitosan nanofibers significantly induced osteopontin, osteocalcin, and alkaline phosphatase (ALP) messenger (m)RNA expression. As to the mechanism, chitosan nanofibers triggered runt-related transcription factor 2 mRNA and protein syntheses. Consequently, results of ALP-, alizarin red-, and von Kossa-staining analyses showed that chitosan nanofibers improved osteoblast mineralization. Taken together, results of this study demonstrate that chitosan nanofibers can stimulate osteoblast proliferation and maturation via runt-related transcription factor 2-mediated regulation of osteoblast-associated osteopontin, osteocalcin, and ALP gene expression.

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Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation

Liao²,

Lin³

et al. 2014

IJN

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show abstract

“…To fulfill the functions of a scaffold in tissue engineering, the scaffold should meet a number of requirements. First, it should have interconnected micropores, so that numerous cells can be seeded, migrate into the inside, increase the cell number and should be supplied by sufficient amounts of nutrients [3]. Micropores make both vascular formation and waste transport possible.…”

Section: Introductionmentioning

confidence: 99%

Polymeric composites containing carbon nanotubes for bone tissue engineering

Sahithi

Swetha

Ramasamy

et al. 2010

International Journal of Biological Macromolecules

148

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“…For example, it has been used to probe 5 μm-wide layers of newly deposited bone [22,23], plaques in Alzheimer's disease [24], and different stages of apoptosis [25]. Other ongoing projects at beamline U10B include studies of the structure of infectious prion proteins in scrapie [26], altered lipid and collagen content and structure in heart disease [27], elevated creatine content in Alzheimer's disease [28], the effects of drug treatment in wound healing [29], polymer distributions in copolymers [30,31], carbon sequestration in soils [32], bioavailability in seeds and plants [16], inclusions in minerals [33], and organic content in IDPs [34] (Figure 3). …”

Section: Technical Reportsmentioning

confidence: 99%