A near infrared BODIPY-based lysosome targeting probe for selectively detection of carboxylesterase 1 in living cells pretreated with pesticides

Ma, Chenggong; Wu, Jichun; Sun, Wen; Hou, Yadan; Zhong, Guoyan; Gao, Rui; Shen, Baolong; Huang, He

doi:10.1016/j.snb.2020.128798

Cited by 24 publications

(15 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Functionalized BODIPYs were synthesized and their molecular structure was fully characterized ( Figure a and Figures S1–S3, Supporting Information). [ 44–50 ] Prior to their application as interlayers in solar cells, theoretical studies were performed in order to reveal the optimized geometrical structures (Figure 1b), energy levels (Table S1, Supporting Information), frontier orbitals (Figure S4, Supporting Information), and molecular dipole moments (Table S2, Supporting Information). The results clearly show that these materials exhibit quite similar energy levels and energy gap values of nearly 3.0 eV with the exception of the methyl ester compound (BDP‐COOCH 3 ), which has an energy gap of 2.2 eV.…”

Section: Resultsmentioning

confidence: 99%

“…Functionalized BODIPYs were synthesized and their molecular structure was fully characterized (Figure 1a and Figures S1-S3, Supporting Information). [44][45][46][47][48][49][50] Prior to their application as interlayers in solar cells, theoretical studies were performed 31+G(d,p) level of theory in gas phase. C/N/B/F/H atoms in grey/blue/pink/light blue/white colors.…”

Section: Properties Of the Interlayersmentioning

confidence: 99%

See 1 more Smart Citation

Functionalized BODIPYs as Tailor‐Made and Universal Interlayers for Efficient and Stable Organic and Perovskite Solar Cells

Soultati

Nunzi

Fakharuddin

et al. 2022

Adv Materials Inter

View full text Add to dashboard Cite

Solar cells based on metal halide perovskite and polymer donor:nonfullerene acceptor blend absorbers have recently witnessed a significant rise in their photovoltaic performance. However, they still suffer from some instability issues originating from the inferior interface quality and poor nanomorphology of the absorber layer. In this work, a series of functionalized boron‐dipyrromethene, BODIPY, molecules are introduced as ultrathin interlayers at the absorber/electron transport layer interface. This study indicates that BODIPY compounds with a high molecular dipole moment can enhance the device performance mainly due to better interface energy level alignment. They also induce passivation of defect traps and improvement in the charge transport properties of the absorber layer coated on top of them. Among the various compounds used, amino‐functionalized BODIPY, owing to the synergetic effect of the abovementioned factors, enables the highest power conversion efficiency in organic (15.69%) as well as in perovskite solar cells (20.12%). Amino‐functionalized BODIPY also demonstrates an enhanced stability under continuous illumination (in nitrogen) without and with heating (at 65 °C) for 1000 h. These results pave the way for the implementation of molecules with tailor‐made functionalities in high efficiency and stable solution‐based photovoltaic devices of the future.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Properties Of the Interlayersmentioning

confidence: 99%

Functionalized BODIPYs as Tailor‐Made and Universal Interlayers for Efficient and Stable Organic and Perovskite Solar Cells

Soultati

Nunzi

Fakharuddin

et al. 2022

Adv Materials Inter

View full text Add to dashboard Cite

show abstract

“…Limit of detection for protamine was calculated as 0.034 μg mL À 1 . [23] Heparin has a high binding affinity for protamine. Therefore, when heparin was added to the CBX/protamine complex, CBX molecules would be dissociated to form protamine resulting in a reduce of fluorescence intensity.…”

Section: Quantitative Determination Of Protaminementioning

confidence: 99%

Sensitive Detection of Protamine Based on a Yellow Emission Fluorophore

Dai

Zhao

et al. 2021

ChemistrySelect

Self Cite

View full text Add to dashboard Cite

In this study, a novel p-hydroxybenzylidene-imidazolidone derivatives-based probe CBX (abbreviation of the synthetic probe) is designed and synthesized for sensitive detection of protamine. CBX emits fluorescence at 569 nm and a large Stokes shift of 114 nm is observed. Since protamine can induce the aggregation of negatively charged CBX, the designed probe shows excellent fluorescent response to protamine. Due to stronger electrostatic interactions between heparin and protamine, protamine prefers to unite with heparin rather than the probe after the addition of heparin, so the fluorescence of CBX decreases and realizes the detection of heparin. Comparing to other reported probes, CBX shows lower background interference, better sensitivity and higher stability. Limit of detection for protamine and heparin is calculated as 0.034 and 0.028 μg mL À 1 , respectively. Moreover, the detection of protamine by CBX is not interfered by other glycosaminoglycans.

show abstract

“…[1,5,7] To address this issue, a few fluorescent chemical tools capable of reporting on CES1 activity in live cells using fluorescence microscopy have been developed. [1,[8][9][10] To date, these tools have been limited in use to study the factors that influence CES1 activity in live cells and the best characterized chemical tools, which have the highest potential utility, have been confined to the BODIPY framework due to their mechanism of reporting on CES1 activity. [1,[8][9][10][11][12] Additionally, these probes require multiple synthetic steps to obtain.…”

Section: Introductionmentioning

confidence: 99%

“…[1,[8][9][10] To date, these tools have been limited in use to study the factors that influence CES1 activity in live cells and the best characterized chemical tools, which have the highest potential utility, have been confined to the BODIPY framework due to their mechanism of reporting on CES1 activity. [1,[8][9][10][11][12] Additionally, these probes require multiple synthetic steps to obtain. We sought to create a design approach that could be applied to a wider range of fluorophores, including those that are commercially available, to generate new fluorescent chemical tools to study CES1.…”

Section: Introductionmentioning

confidence: 99%

Carbonate‐Based Fluorescent Chemical Tool for Uncovering Carboxylesterase 1 (CES1) Activity Variations in Live Cells

et al. 2022

View full text Add to dashboard Cite

Carboxylesterase 1 (CES1) plays a key role in the metabolism of endogenous biomolecules and xenobiotics including a variety of pharmaceuticals. Despite the established importance of CES1 in drug metabolism, methods to study factors that can vary CES1 activity are limited with only a few suitable for use in live cells. Herein, we report the development of FCP1, a new CES1 specific fluorescent probe with a unique carbonate substrate constructed from commercially available reagents. We show that FCP‐1 can specifically report on endogenous CES1 activity with a robust fluorescence response in live HepG2 cells through studies with inhibitors and genetic knockdowns. Subsequently, we deployed FCP‐1 to develop a live cell fluorescence microscopy‐based approach to identify activity differences between CES1 isoforms. To the best of our knowledge, this is the first application of a fluorescent probe to measure the activity of CES1 sequence variants in live cells.

show abstract

A near infrared BODIPY-based lysosome targeting probe for selectively detection of carboxylesterase 1 in living cells pretreated with pesticides

Cited by 24 publications

References 44 publications

Functionalized BODIPYs as Tailor‐Made and Universal Interlayers for Efficient and Stable Organic and Perovskite Solar Cells

Functionalized BODIPYs as Tailor‐Made and Universal Interlayers for Efficient and Stable Organic and Perovskite Solar Cells

Sensitive Detection of Protamine Based on a Yellow Emission Fluorophore

Carbonate‐Based Fluorescent Chemical Tool for Uncovering Carboxylesterase 1 (CES1) Activity Variations in Live Cells

Contact Info

Product

Resources

About