A Near-Infrared-Triggered Dynamic Wrinkling Biointerface for Noninvasive Harvesting of Practical Cell Sheets

Abstract

Cell sheet engineering represents a new era of precise and efficient regenerative medicine, but its efficacy is limited by the elaborative preparation and the weak mechanics. Herein, a near-infrared (NIR)-triggered dynamic wrinkling biointerface was designed for rapid acquisition of practical cell sheets. The biocompatible NIR can initiate the photothermal-mechanical linkage cascade to efficiently dissolve the collagen supporting layer and release the high-quality cell sheets. The interfacial shear force generates with the dynamic wrinkling, playing an active role in accelerating the cell sheet release. High-quality and self-supporting cell sheets can be harvested within a few minutes, demonstrating a new paradigm of photothermal-mechanical manipulation. The transplantable cell sheets with outstanding physiological and mechanical performances were proven to promote wound healing in skin regeneration. This method may open a completely new front in thermal and mechanical responsive cascade to harvest cell sheets, facilitating their wide applications in regenerative medicine.

Mechanical behavior of biomimetic oriented cell sheets from a perspective of living materials

“…1a). 26 The dynamic wrinkle layer was fabricated via spin-Scheme 1 Harvesting and mechanical testing of oriented cell sheets (OCSs). The OCSs were cultured on sandwich-type micropatterned substrates and harvested under NIR irradiation based on a photothermal-mechanical manipulation method.…”

“…21,25 Herein, we have first developed a sandwichtype micropatterned substrate to create OCSs based on a photothermal-mechanical manipulation method. 26 The sandwich-type substrate consists of a micropatterned thermal phase-transferring collagen layer, dynamic wrinkle layer, and photothermal conversion layer. On the micropatterned substrate, aligned cell sheets with different degrees of orientation were incubated.…”

Therapeutic potential of dental pulp stem cells and their derivatives: Insights from basic research toward clinical applications

“…DPSC sheets have also been reported to be composed of multilayer cells forming after 4 wk of culture in basal medium[ 117 ]. In addition to the above cell sheet preparation techniques, more recently, scholars have also used techniques such as near infrared triggering, light induction, and the combination of rough surfaces with thermoresponsive polymers to accelerate or improve the formation of cell sheets[ 118 - 120 ]. These techniques need to be further applied to the preparation of DPSC sheets.…”

Strong and tough self-wrinkling polyelectrolyte hydrogels constructed via a diffusion–complexation strategy

“…15,16 To recreate the distinctive performance of the natural self-wrinkling regime, various elaborate artificial self-wrinkling systems have been successfully fabricated by modulating the stress distribution of hard skin on soft matrices. [17][18][19][20][21][22][23][24] Among others, self-wrinkling hydrogels featuring tissue-like mechanical properties and good biocompatibility have attracted tremendous attention owing to their promising applications in tissue scaffolds, 1,25 bionic mucosal tissue, 26 sensor devices, [27][28][29][30][31] soft robotics, 26 etc.…”