A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

Mesbah, Kiarach; Duc, Thanh; Jensen, Thomas Glasdam; Solá, Laura; Chiari, Marcella; Kutter, Jörg Peter; Taverna, Myriam

doi:10.1007/s00604-016-1825-4

Cited by 19 publications

(23 citation statements)

References 30 publications

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“…Thiol-ene substrates have been used in microfluidic techniques recently due to properties including good solvent resistance, tunable rigidity, rapid polymerization kinetics, good optical transparency, excellent chemical resistance to solvents, good adhesion to glass and metal, and temperature resistance (from -150°C to 125°C). 106, 107 The material was introduced for microchip electrophoresis separations recently, 107 and thiol-ene microchip electrophoresis involving microchannel surface modification via neutral copolymers for protein separations was performed. 106 In this work, the initial SU-8 mold fabrication step is performed in a cleanroom.…”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

“…106, 107 The material was introduced for microchip electrophoresis separations recently, 107 and thiol-ene microchip electrophoresis involving microchannel surface modification via neutral copolymers for protein separations was performed. 106 In this work, the initial SU-8 mold fabrication step is performed in a cleanroom. The casting of a PDMS mold, fabrication of thiol-ene microchannel and cover layers, and bonding of the thiol-ene layers is performed outside of the cleanroom.…”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

“…The insert in the figure displays electropherograms from five repetitive separations of TI-Alexa 488 (1.5 μM) and BSA-FITC (3 μM). (Modified from reference 106 with permission)…”

Section: Figurementioning

confidence: 99%

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Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

Analyst

120

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This review article describes the significant recent advances in analysis of proteins by capillary and microchip electrophoresis during the period mid 2014 to early 2017. The review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. Protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.

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Section: Separation Modes and Conditionsmentioning

confidence: 99%

Section: Separation Modes and Conditionsmentioning

confidence: 99%

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Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

Analyst

120

View full text Add to dashboard Cite

show abstract

“…The mechanical stiffness and rigidity of thiol-enes can also be tuned by altering the respective quantities of the thiol and allyl ("ene") monomers in the bulk material. [18] The use of off-stoichiometric monomer ratios results in excess of free thiol or allyl functional groups on the polymer surface [19][20][21] which have been exploited for numerous biofunctionalizations [22][23][24][25] and for aqueous MCE in combination with fluorescence detection. [19,22,26] Thiol-ene channels generally maintain high cathodic electroosmotic flow over a wide pH range (pH 3-12) [19,22] and show little nonspecific adsorption of peptides in native, allyl-rich microchannel walls.…”

Section: Introductionmentioning

confidence: 99%

“…[19] Polyacrylate copolymer coatings can be used to eliminate protein adsorption in thiol-rich microchannels. [22] Thanks to their inherent good stability against organic solvents, thiol-enes as chip fabrication materials also provide greater flexibility in terms of analytical method development than most other microfabrication polymers. In this study, we exploit the good solvent compatibility to carry out microchip electrophoresis in non-aqueous conditions.…”

Section: Introductionmentioning

confidence: 99%

Aqueous and non-aqueous microchip electrophoresis with on-chip electrospray ionization mass spectrometry on replica-molded thiol-ene microfluidic devices

Tähkä

Bonabi

Jokinen

et al. 2017

Journal of Chromatography A

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This work describes aqueous and non-aqueous capillary electrophoresis on thiol-ene-based microfluidic separation devices that feature fully integrated and sharp electrospray ionization (ESI) emitters. The chip fabrication is based on simple and low-cost replica-molding of thiol-ene polymers under standard laboratory conditions. The mechanical rigidity and the stability of the materials against organic solvents, acids and bases could be tuned by adjusting the respective stoichiometric ratio of the thiol and allyl ("ene") monomers, which allowed us to carry out electrophoresis separation in both aqueous and non-aqueous (methanol- and ethanol-based) background electrolytes. The stability of the ESI signal was generally ≤10% RSD for all emitters. The respective migration time repeatabilities in aqueous and non-aqueous background electrolytes were below 3 and 14% RSD (n=4-6, with internal standard). The analytical performance of the developed thiol-ene microdevices was shown in mass spectrometry (MS) based analysis of peptides, proteins, and small molecules. The theoretical plate numbers were the highest (1.2-2.4×10m) in ethanol-based background electrolytes. The ionization efficiency also increased under non-aqueous conditions compared to aqueous background electrolytes. The results show that replica-molding of thiol-enes is a feasible approach for producing ESI microdevices that perform in a stable manner in both aqueous and non-aqueous electrophoresis.

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An integrated plastic microchip for enhancing electrophoretic separation using tunable pressure‐driven backflows

et al. 2022

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Microfluidic CE (MCE) is an effective solution for rapid and sensitive determination of multiple analytes. Herein, a dynamic coated cyclic olefin copolymer microchip was developed having an on‐chip micropump for fluid velocity adjusting in electrophoretic separations. This micropump was fabricated by constructing a polyacrylamide gel membrane at one channel terminal. Once applying electric field across the membrane, a pressure‐driven flow generated automatically to balance the electroosmotic flow (EOF) mismatch at the channel‐membrane interface. The influence of gel precursor concentration and operating voltages on the fluid velocity was carefully evaluated. Moreover, the highly integration of injection, separation, and pumping units of the MCE system minimized the dead volume and provides satisfied column efficiency. Experiments showed that by adjusting of pumping voltage reduced the fluid velocity by a factor of 6, resulting six‐ and threefold resolving power enhancements of rhodamine dye mixture and amino acid mixture, respectively. Furthermore, the developed MCE method was applied for rhodamines and amino acids quantitation in food and cosmetics, with standard addition recoveries of 87.3–106.9% and 89.9–117.4%, respectively. These results were also confirmed by standard HPLC method, revealing the application potential in fast and onsite analysis of complex samples.

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A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

Cited by 19 publications

References 30 publications

Recent advances in protein analysis by capillary and microchip electrophoresis

Recent advances in protein analysis by capillary and microchip electrophoresis

Aqueous and non-aqueous microchip electrophoresis with on-chip electrospray ionization mass spectrometry on replica-molded thiol-ene microfluidic devices

An integrated plastic microchip for enhancing electrophoretic separation using tunable pressure‐driven backflows

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