1990
DOI: 10.1128/jvi.64.12.6176-6183.1990
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A neutralizable epitope common to the envelope glycoproteins of ecotropic, polytropic, xenotropic, and amphotropic murine leukemia viruses

Abstract: An epitope common to all classes of murine leukemia viruses (MuLVs) was detected by reactivity of MuLVs with a rat monoclonal antibody (MAb) termed 83A25. The antibody is of the immunoglobulin G2a isotype and was derived after fusion of NS-1 myeloma cells with spleen cells from a Fischer rat immunized with a Friend polytropic MuLV. The antibody reacted with nearly all members of the ecotropic, polytropic, xenotropic, and amphotropic classes of MuLVs. Unreactive viruses were limited to the Friend ecotropic MuLV… Show more

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Cited by 160 publications
(61 citation statements)
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References 56 publications
(55 reference statements)
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“…L. Portis (National Institutes of Health, Hamilton, Montana) . The anti-gp70 mAb 83A25 (46) recognizing an epitope that is present on gp70 of all classes of murine leukemia viruses (MuLds), was used as a positive control . Staining was performed by incubating infected and uninfected cells with serial dilutions of serum samples, followed by incubation with FITC-labeled goat anti-mouse IgG + IgM .…”
Section: Methodsmentioning
confidence: 99%
“…L. Portis (National Institutes of Health, Hamilton, Montana) . The anti-gp70 mAb 83A25 (46) recognizing an epitope that is present on gp70 of all classes of murine leukemia viruses (MuLds), was used as a positive control . Staining was performed by incubating infected and uninfected cells with serial dilutions of serum samples, followed by incubation with FITC-labeled goat anti-mouse IgG + IgM .…”
Section: Methodsmentioning
confidence: 99%
“…FITCconjugated anti-CD2, PE-conjugated anti-CD20, and their isotypematched controls were obtained from Coulter Immunology (Hialeah, FL); PE-conjugated anti-CD4 along with its isotype matched control were obtained from Becton Dickinson & Co. (Mountain View, CA); and FITC-conjugated anti-CD8 along with its isotypematched control were obtained from Gen Trak, Inc. (Plymouth Meeting, PA). The rat mAb, 83A25 (32,33), was used in these studies to recognize an epitope of the envelope gp70 molecule common to the different classes of murine leukemia retroviruses. The rat IgG2a isotype control was obtained from Zymed (San Francisco, CA).…”
Section: Culture Of Bone Marrow Cells Immunoselected Cd34 + Cellsmentioning
confidence: 99%
“…At 48 h posttransfection, the cells were harvested with enzyme-free cell dissociation solution (GIBCO-BRL) and washed with phosphate-buffered saline (PBS). The cells (10 6 ) were incubated at 4°C for 1 h with the rat monoclonal antibody 83A25 (10), which recognizes the C terminus of MoMuLV gp70 envelope protein. The cells were washed with 10% goat serum in PBS and incubated with fluorescein isothiocyanate-labeled goat anti-rat IgG (Kirkegaard & Perry Laboratories, Inc., Gaithersburg, Md.)…”
Section: Methodsmentioning
confidence: 99%