A New 15–50 MHz Array-Based Micro-Ultrasound Scanner for Preclinical Imaging

Foster, F. Stuart; Mehi, J.; Lukacs, Marc; Hirson, D.; White, C Michael; Chaggares, Chris; Needles, A.

doi:10.1016/j.ultrasmedbio.2009.04.012

Cited by 169 publications

(92 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A high-frequency ultrasound imaging system (Vevo 2100, VisualSonics Inc., Toronto, Canada) with a 30 MHz linear array transducer was used. 29 During ultrasound imaging, mice were anesthetized using isoflurane at 1.5% in 21% O 2 , and body temperature was maintained at 36-37 C. The heart rate and respiration rate were continuously monitored. For evaluating the cardiac function, M-mode recordings of the left ventricle were made and analyzed for wall thicknesses and chamber dimensions.…”

Section: Measurement Of Cbf Using Continuous Arterial Spin Labellingmentioning

confidence: 99%

Functional and anatomical evidence of cerebral tissue hypoxia in young sickle cell anemia mice

Cahill

Gazdzinski

Tsui

et al. 2016

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

Cerebral ischemia is a significant source of morbidity in children with sickle cell anemia; however, the mechanism of injury is poorly understood. Increased cerebral blood flow and low hemoglobin levels in children with sickle cell anemia are associated with increased stroke risk, suggesting that anemia-induced tissue hypoxia may be an important factor contributing to subsequent morbidity. To better understand the pathophysiology of brain injury, brain physiology and morphology were characterized in a transgenic mouse model, the Townes sickle cell model. Relative to age-matched controls, sickle cell anemia mice demonstrated: (1) decreased brain tissue pO 2 and increased expression of hypoxia signaling protein in the perivascular regions of the cerebral cortex; (2) elevated basal cerebral blood flow , consistent with adaptation to anemia-induced tissue hypoxia; (3) significant reduction in cerebrovascular blood flow reactivity to a hypercapnic challenge; (4) increased diameter of the carotid artery; and (5) significant volume changes in white and gray matter regions in the brain, as assessed by ex vivo magnetic resonance imaging. Collectively, these findings support the hypothesis that brain tissue hypoxia contributes to adaptive physiological and anatomic changes in Townes sickle cell mice. These findings may help define the pathophysiology for stroke in children with sickle cell anemia.

show abstract

Section: Measurement Of Cbf Using Continuous Arterial Spin Labellingmentioning

confidence: 99%

Functional and anatomical evidence of cerebral tissue hypoxia in young sickle cell anemia mice

Cahill

Gazdzinski

Tsui

et al. 2016

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

show abstract

“…14 The abdom-inal aorta is also easily visualized. With pulsed wave Doppler and newly available Doppler color flow imaging, 17 the flow patterns in the mouse aorta can be comprehensively observed.…”

mentioning

confidence: 99%

Aortic Regurgitation Dramatically Alters the Distribution of Atherosclerotic Lesions and Enhances Atherogenesis in Mice

Zhou

Zhu

Foster

et al. 2010

ATVB

Self Cite

View full text Add to dashboard Cite

Objective-Hemodynamics plays a critical role in atherogenesis, but the association between flow pattern and preferential localization of lesion is not fully understood. We developed a mouse model of aortic valve regurgitation (AR) to change the aortic flow pattern and observed the effects on plaque formation. Methods and Results-High-frequency Doppler ultrasound imaging of 10 untreated C57BL/6J mice and 6 sham-treated low-density lipoprotein receptor-deficient (Ldlr Ϫ/Ϫ ) mice revealed consistent antegrade blood flow throughout the aorta and oscillatory flow only along the lesser curvature of the aortic arch. Catheter-induced AR in 7 Ldlr Ϫ/Ϫ mice produced various degrees of diastolic retrograde flow throughout the aorta. After the mice were fed a cholesterol-enriched diet for 6 weeks, the burden of atherosclerotic lesions was increased 6-fold, with the naturally plaque-resistant descending aorta becoming susceptible. The AR severity correlated positively with the lesion burden in the descending thoracic and abdominal aorta but negatively with the lesions in the ascending aorta and aortic arch. Key Words: atherosclerosis Ⅲ blood flow Ⅲ Doppler ultrasound Ⅲ genetically altered mice C omplex and disturbed blood flow at sites of aortic curvature, branches, bifurcations, or other arterial regions is a predisposing factor for atherosclerosis. 1,2 Endothelial cells of arteries sense wall shear stress (WSS), the frictional force of blood flow, and respond differently to various flow patterns by triggering unique signal transduction pathways that lead to distinct gene expression patterns. Unidirectional laminar WSS diminishes endothelial inflammatory responses and protects the artery from atherosclerosis. In contrast, low time-averaged WSS with oscillating directions correlates with increased predisposition to atherosclerosis. 3,4 However, the association between flow dynamics and atherogenesis is still not fully understood, and the roles of low WSS and oscillatory WSS have never been differentiated. Useful insights have been gained from previous studies on animals of various species by altering the blood flow through a variety of surgical interventions. 4 However, those models focus on specific aspects of endothelial cell biology in limited vessel segments and are unable to provide, at the systemic level, an in vivo environment with flow alteration in a morphologically normal aortic system for a comprehensive elucidation of the flow-plaque relationship. Conclusion-ThisIn the last decade, mice have commonly been used in biological research because this species is most amenable to genetic manipulation. Low-density lipoprotein receptor-deficient (Ldlr Ϫ/Ϫ ) and apolipoprotein E-deficient (Apoe Ϫ/Ϫ ) mice are the most popular strains for studies related to atherosclerosis. 5-9 A model was developed by placing a cast around the common carotid artery of Apoe Ϫ/Ϫ mice, in an attempt to induce 3 regions of lowered, increased, and oscillatory WSS. 10,11 The segment facing elevated WSS was spared from lesions. However, the proxi...

show abstract

“…arterioles and venules larger than about 50 mm. Despite this, the development of high frequency micro-US [156,157] and Doppler, has facilitated the non-invasive imaging of microscopic structure and blood flow with improved spatial resolution. The intensity of scatter echoes depends on the reflective property of the tracers [4].…”

Section: Ultrasoundmentioning

confidence: 99%

‘Go with the flow ’: A review of methods and advancements in blood flow imaging

Daly

Leahy

2012

Journal of Biophotonics

110

108

View full text Add to dashboard Cite

Physics has delivered extraordinary developments in almost every facet of modern life. From the humble thermometer and stethoscope to X‐Ray, CT, MRI, ultrasound, PET and radiotherapy, our health has been transformed by these advances yielding both morphological and functional metrics. Recently high resolution label‐free imaging of the microcirculation at clinically relevant depths has become available in the research domain. In this paper, we present a comprehensive review on current imaging techniques, state‐of‐the‐art advancements and applications, and general perspectives on the prospects for these modalities in the clinical realm. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

A New 15–50 MHz Array-Based Micro-Ultrasound Scanner for Preclinical Imaging

Cited by 169 publications

References 22 publications

Functional and anatomical evidence of cerebral tissue hypoxia in young sickle cell anemia mice

Functional and anatomical evidence of cerebral tissue hypoxia in young sickle cell anemia mice

Aortic Regurgitation Dramatically Alters the Distribution of Atherosclerotic Lesions and Enhances Atherogenesis in Mice

‘Go with the flow ’: A review of methods and advancements in blood flow imaging

Contact Info

Product

Resources

About