A New 24-membered Lactone and a New Polyene δ-Lactone from the Marine Bacterium Bacillus marinus

Xue, Chunmei; Tian, Li; Xu, Min-Juan; Deng, Zhiwei; Lin, Wenhan

doi:10.1038/ja.2008.94

Cited by 51 publications

(36 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fermentation broths of B-9987/sfp followed by extraction and HPLC analysis showed that overexpression of sfp led to increment of the macrolactin A production to 29 mg L 1 ( Figure 4A, panel iii), which is about 1.6-fold as compared to that of the wild type strain (18 mg L 1 ) ( Figure 4A, panel ii). The identity of macrolactin A was determined by comparison with the standard [5] (Figure 4A, panel i), exhibiting the same retention time and UV spectrum ( Figure 4B) Figure 4C). As a result, the biocontrol efficacy of B-9987/sfp could probably be enhanced, indicating this strategy would potentially be applicable to improvement of other biocontrol strains.…”

Section: Overexpression Of the Phosphopantetheinyl Transferase Gene Smentioning

confidence: 99%

“…Bacillus marinus B-9987 was isolated from the rhizosphere of Suaeda salsa collected in the intertidal zone of Bohai Bay of Eastern China [2]. Chemical investigations revealed that B. marinus B-9987 is capable to produce a variety of antimicrobial secondary metabolites [35], such as macrolactins (Figure 1), which are able to inhibit the growth of plant fungal pathogens Pyricularia oryzae and Alternaria solani by destroying their cell walls [5]. Besides antifungal activity, macrolactins also exhibit antibacterial, antiviral, anticancer, anti-inflammatory, and as well as antiangiogenic activities [69].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain

Liu

Zheng

Zhan

et al. 2014

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

Marine-derived Bacillus strains have been proved to be a very promising source for natural product leads. However, transformation of environmental strains is much more difficult than that of domesticated strains. Here, we report the development of an efficient and robust electroporation-based transformation system for marine-derived Bacillus marinus B-9987, which is a macrolactin antibiotics producer and a very promising biological control agent against fungal plant diseases. The transformation efficiency was greatly enhanced 10 3 -fold by using unmethylated plasmid to bypass modification-restriction barrier, and using glycine betaine to protect cells from electrical damages during electroporation. Addition of HEPES and 2 mmol L 1 MgCl 2 further improved the efficiency by additional 2-fold, with a maximum value of 7.1×10 4 cfu/µg pHT3101. To demonstrate the feasibility and efficiency of the protocol, a green fluorescent protein reporter system was constructed; furthermore, phosphopantetheinyl transferase gene sfp, which is essential to the biosynthesis of polyketides and nonribosomal peptides, was overexpressed in B-9987, leading to increased production of macrolactin A by about 1.6-fold. In addition, this protocol is also applicable to marine-derived Bacillus licheniforms EI-34-6, indicating it could be a reference for other undomesticated Bacillus strains. To our knowledge, this is the first report regarding the transformation of marine-derived Bacillus strain. marine-derived, Bacillus marinus, electroporation, macrolactin, green fluorescent protein, phosphopantetheinyl transferase Citation:Liu Y, Zheng H, Zhan GH, Qin W, Tian L, Li WL. Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain.

show abstract

Section: Overexpression Of the Phosphopantetheinyl Transferase Gene Smentioning

confidence: 99%

mentioning

confidence: 99%

Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain

Liu

Zheng

Zhan

et al. 2014

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The structures of 3-5 were established by analyzing their 1D NMR spectral data and by comparison with the literature. 5,6 Compound 1 had the molecular formula of C 24 H 34 O 6 as deduced from NMR spectra and HR-ESI-MS. 13 ) were obviously observed in the NMR spectra of 1, which should be caused by the change of the three-dimensional effect at C-7 position, and suggested that the configuration of ÀOH at C-7 in 1 should be a-configuration instead of b-configuration as it existed in 2 and other previously isolated macrolactins. 4,[7][8][9][10][11][12][13] The suggestion was supported by the NOESY (Figure 3), in which NOE correlations of H-6b with H-8/H-4 and H-7 with H-8 were observed, whereas no NOE correlations between H-7 and H-9/H-5 were observed, which were in agreement with the MM2 optimized conformation of 1.…”

mentioning

confidence: 85%

“…4,[7][8][9][10][11][12][13] Some of these compounds showed strong antibacterial, anti-tumor, and antivirus activities. 4,7,[9][10][11][12][13] The antibacterial activity of 1-5 against four bacterial strains and the antilarval activity of 3-5 against larval settlement of Bugula neritina and Balanus amphitrite larvae were evaluated. This paper deals with the isolation, structural elucidation of 1 and biological activity of 1-5.…”

mentioning

confidence: 99%

Antibacterial and antilarval compounds from marine gorgonian-associated bacterium Bacillus amyloliquefaciens SCSIO 00856

Gao

Tian

et al. 2010

J Antibiot

View full text Add to dashboard Cite

It is well understood that corals harbor diverse microbial communities. Recently, many coral-associated bacteria have been characterized as sources of marine natural products, especially as the coral surface is more nutrient rich than seawater or even sediments. 1,2 Colonization of coral surfaces by bacteria and other microorganisms is mostly nondestructive to corals. Owing to the close spatial vicinity of these biofilm-forming bacteria, it can be expected that the indigenous microbial population is adapted to competitive conditions. 3 The production of secondary metabolites is a common adaptation of these bacteria to compete in such microenvironments. There have been few reports documented on exploration of coralassociated bacteria in the South China Sea as a source of secondary metabolites.In order to obtain biologically active natural products from coral-associated bacteria, we studied the marine bacterium Bacillus amyloliquefaciens SCSIO 00856 isolated from the South China Sea gorgonian Junceella juncea. In the preliminary experiments, we found that the culture broth of the strain had strong antibacterial activity towards three bacteria (Escherichia coli, Bacillus subtilis, and Staphyloccocus aureus) and antilarval activity towards laboratory-reared bryozoan Bugula neritina larvae. In this study, a new 24-membered ring lactone, macrolactin V (1) (Figure 1), together with four known compounds, macrolactin S (2), 4 4-butoxyphenol (3), 5 4-propoxyphenol (4), 6 and 4-ethoxyphenol (5), 6 was isolated from a culture broth of the strain. Macrolactins are polyene macrolides containing a 24-membered lactone ring. About 20 macrolactins have been chemically characterized, including macrolactins A-U, 7-O-succinyl macrolactin A, and 7-O-succinyl macrolactin F, from marine Bacillus sp. sediment isolate, and 7-O-malonyl macrolactin A from Bacillus subtilis soil isolate. 4,7-13 Some of these compounds showed strong antibacterial, anti-tumor, and antivirus activities. 4,7,[9][10][11][12][13] The antibacterial activity of 1-5 against four bacterial strains and the antilarval activity of 3-5 against larval settlement of Bugula neritina and Balanus amphitrite larvae were evaluated. This paper deals with the isolation, structural elucidation of 1 and biological activity of 1-5.The producing strain, Bacillus amyloliquefaciens SCSIO 00856, was isolated from the gorgonian coral Junceella juncea in Sanya, Hainan province, China. The strain culture (2 ml) was used to inoculate 50 ml seed medium consisting of 0.4% yeast extract, 0.4% glucose, 0.5% malt meal, and 1.8% sea salt (pH 7.3), in a 250-ml flask. A volume of 20 ml of the resulting culture was used to inoculate 400 ml of the seed medium, in a 1-l flask. The culture was incubated at 27 1C on a rotary shaker operating at 150 r.p.m. for 5 days. The resulting 400-ml culture was used to inoculate 120 l of production medium, in a 200-l fermenter, containing the following components per liter of water: yeast extract 4 g, malt meal 5 g, glucose 4 g, and sea salt 18 g. The production fe...

show abstract

“…1,2) The strain Bacillus marinus B-9987, isolated from Suaeda salsa in Bohai coastline of P. R. China, has shown excellent activity against phytopathogens both in vitro and in vivo, 3,4) and some antimicrobial macrolactin constituents 5) have been isolated from this strain. In order to screen more constituents by bio-guided isolation, we have obtained two new cyclic lipopeptides maribasins A (1) and B (2) (Fig.…”

mentioning

confidence: 99%

Two New Antifungal Cyclic Lipopeptides from Bacillus marinus B-9987

Zhang

Liu

et al. 2010

CHEMICAL & PHARMACEUTICAL BULLETIN

Self Cite

View full text Add to dashboard Cite

show abstract

A New 24-membered Lactone and a New Polyene δ-Lactone from the Marine Bacterium Bacillus marinus

Cited by 51 publications

References 9 publications

Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain

Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain

Antibacterial and antilarval compounds from marine gorgonian-associated bacterium Bacillus amyloliquefaciens SCSIO 00856

Two New Antifungal Cyclic Lipopeptides from Bacillus marinus B-9987

Contact Info

Product

Resources

About