A new 24-membered macrolide shows insecticidal activity against Pieris rapae potentially through induction of programmed cell death

Abstract

Pieris rapae is one of the most destructive pests to cabbage around the world and it has developed resistance to present pesticides. 5 ′-Epi-SPA-6952A was a new 24-membered macrolide isolated from the cultured broth of Streptomyces diastatochromogenes. The aim of this study was to confirm its insecticidal activity against Pieris rapae in field condition, and investigate its potential active mechanism in the cellular level. The results of field experiments showed that the control effects for Pieris rapae reached 79.33% at 45 g/hm 2 after 24 h. Meanwhile, we demonstrated that 5 ′-Epi-SPA-6952A could inhibit the proliferation of Sf9 cells, induce the DNA damage and enhance the programmed cell death. Intracellular biochemical assay indicated that the cytotoxicity of 5 ′-Epi-SPA-6952A may be associated with the activity of mitochondrial apoptotic pathways or AMPK/mTOR-mediated autophagy. In conclusion, this study suggests a possible application of 5 ′-Epi-SPA-6952A as a potential insecticide against Pieris rapae in agriculture.

Effects of different levels of cottonseed meal on laying performance, egg quality, intestinal immunity and hepatic histopathology in laying hens

“…Autophagy is a stress reaction that arises when cells resist the undesirable external stimulus. The excessive autophagy impairs the morphology and functions of cells (Liu et al, 2018;Zhang et al, 2019). The ingestion of gossypol present in cottonseed and its products (cake and meal) may promote clinical poisoning of liver damage (Mena et al, 2004).…”

Isoorientin attenuates benzo[a]pyrene-induced liver injury by inhibiting autophagy and pyroptosis in vitro and vivo

“…Autophagy is a stress reaction that arises when cells resist the undesirable external stimulus. The excessive autophagy impairs the morphology and functions of cells (Cui, Liu, & Zhang, 2018;Zhang et al, 2019) and results in the autophagic cell death (Sergi & Jordi, 2018). BaP could induce the liver dysfunction by increasing the appearance of vesicles containing degraded cellular material (Gorria et al, 2008).…”