A new apparatus for standardization of experimental burn models

Arda, Mehmet Sürhan; Koçman, Atacan Emre; Soztutar, Erdem; Baksan, Bedri; Çetin, Cengiz

doi:10.1016/j.burns.2017.01.014

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…In comparison to the animal model, more superficial burn depths have been noted on ex vivo HS, although there was no difference between the steps of experimental model (15). And it was realized that for Groups 1 and 6, variable depths of second degree burns were created as in the animal model.…”

Section: Discussionmentioning

confidence: 98%

“…However, infeasible real-time contact temperatures and applied weight force (WF) measurements were the weak points of these models (3,14). For this reason, in 2016 we designed a standardized contact burn model in which the real-time contact temperature and pressure could be controlled (15). A standardized second degree burn can be achieved in rats using custom designed apparatus (15).…”

Section: Introductionmentioning

confidence: 99%

“…For this reason, in 2016 we designed a standardized contact burn model in which the real-time contact temperature and pressure could be controlled (15). A standardized second degree burn can be achieved in rats using custom designed apparatus (15). However, although the created wounds were second degree, the burn percentages were significantly different from each other.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Burn Depth at Different Temperatures on Ex Vivo Human Skin with Standardized Model and Comparison of the Results with Rat Contact Burn Model

Arda¹,

Kuas²,

Soztutar³

et al. 2018

jpr

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Burn Depth at Different Temperatures on Ex Vivo Human Skin with Standardized Model and Comparison of the Results with Rat Contact Burn Model

Arda¹,

Kuas²,

Soztutar³

et al. 2018

jpr

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“…The major advantage of the device used in this study consists of the automatic turn off of the vector, doubled by an immediate cooling down process of the skin with the help of cool water sent through the pipe system. Additionally, the steam's temperature is directly measured as it is expelled from the silicon head and not from the heating tub as in other experimental models [ 28 , 32 , 36 , 38 ]. Another major, beneficial advantage, in comparison to other devices [ 18 , 20 ], is that because the heating element itself is not a hard surface that comes in contact with the rat's skin it ensures lesion uniformity via heat radiation from the steam.…”

Section: Discussionmentioning

confidence: 99%

Design and Testing of an Experimental Steam-Induced Burn Model in Rats

Porumb

Trandabat

Terinte

et al. 2017

BioMed Research International

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Background Most of the current models for experimental burns pose difficulties in ensuring consistency and standardization. Aim of Study We aimed to develop an automated, reproducible technique for experimental burns using steam-based heat transfer. Methods The system developed for steam exposure was based on a novel, integrated, computer-controlled design. Three groups of rats were exposed to steam for 1, 3, and 7 seconds. The lesions were evaluated after 20 minutes, 48 hours, and 72 hours after burn induction. Results One-second steam application produced a superficial second-degree burn; three-second application induced deep second-degree burn; and seven-second application led to a third-degree burn. Conclusion The high level of automation of our integrated, computer-controlled system makes the difference between our system and other models, by ensuring the control of the duration of exposure, temperature, and pressure and eliminating as many potential human generated errors as possible. The automated system can accurately reproduce specific types of burns, according to histological assessment. This model could generate the reproducible data needed in the study of burn pathology and in order to assess new treatments.

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Experimental Models for Controlled Burn Injuries in Rats: A Systematic Analysis of Original Methods and Burn Devices

Morărașu

Morarasu

Gheţu

et al. 2021

Journal of Burn Care &Amp; Research

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AIM Despite a wide variety of models found in literature, choosing the right one can be difficult as many of them are lacking precise methodology. This study aims to analyze and compare original burn models in terms of burn device and technique, parameters, and wound depth assessment. METHODS A systematic search was performed according to PRISMA guidelines on studies describing original experimental burn models in rats. The adapted PICO formula and ARRIVE checklist were followed for inclusion and assessment of quality of studies. Characteristics of animals, burn technique, burn parameters and method of histological confirmation of burn depth were recorded. RESULTS Twenty-seven studies were included in the final analysis. Most studies used direct contact with skin for burn infliction (n=20). The rat’s dorsum was the most common site (n=18). Ten studies used manually controlled burn devices, while ten designed automatic burn devices with control over temperature (n=10), exposure time (n=5), and pressure (n=5). Most studies (n=7) used a single biopsy taken from the center of the wound to confirm burn depth immediately after burn infliction. CONCLUSION From the wide variety of burn models in current literature, our study provides an overview of the most relevant experimental burn models in rats aiding researchers to understand what needs to be addressed when designing their burn protocol. Models cannot be compared as burn parameters variate significantly. Assessment of burn depth should be done in a standardized, sequential fashion in future burn studies to increase reproducibility.

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A new apparatus for standardization of experimental burn models

Cited by 4 publications

References 16 publications

Comparison of Burn Depth at Different Temperatures on Ex Vivo Human Skin with Standardized Model and Comparison of the Results with Rat Contact Burn Model

Comparison of Burn Depth at Different Temperatures on Ex Vivo Human Skin with Standardized Model and Comparison of the Results with Rat Contact Burn Model

Design and Testing of an Experimental Steam-Induced Burn Model in Rats

Experimental Models for Controlled Burn Injuries in Rats: A Systematic Analysis of Original Methods and Burn Devices

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