Design and Testing of an Experimental Steam-Induced Burn Model in Rats

Abstract

Background Most of the current models for experimental burns pose difficulties in ensuring consistency and standardization. Aim of Study We aimed to develop an automated, reproducible technique for experimental burns using steam-based heat transfer. Methods The system developed for steam exposure was based on a novel, integrated, computer-controlled design. Three groups of rats were exposed to steam for 1, 3, and 7 seconds. The lesions were evaluated after 20 minutes, 48 hours, and 72 hours after burn induction. Results One-second steam application produced a superficial second-degree burn; three-second application induced deep second-degree burn; and seven-second application led to a third-degree burn. Conclusion The high level of automation of our integrated, computer-controlled system makes the difference between our system and other models, by ensuring the control of the duration of exposure, temperature, and pressure and eliminating as many potential human generated errors as possible. The automated system can accurately reproduce specific types of burns, according to histological assessment. This model could generate the reproducible data needed in the study of burn pathology and in order to assess new treatments.

The Effects of Idazoxan and Efaroxan Improves Memory and Cognitive Functions in Rats Experimental research

“…There are further investigations of related studies that indicated the role of substances acting on the imidazoline receptors, on behavior, remembrance, eagerness to learn, locomotor activity and sedation. Finally, progress will also depend on the means of understanding the structural and functional organization of the receptors in the rat cerebral cortex from an interdisciplinary perspective, including psychiatry, psychology, behavioral sciences, genetics, and other neurosciences [8][9][10]. Idazoxan and Efaroxan have a certain potential for being used in new drug development based on the results that have been reported after conducting numerous behavioral animal models, but their clinical use is not implemented yet.…”

Wound healing prospective of pongamia glabra, piper nigrum and momordica charantia on albino rats using anemic burn wound model

“…The mean epithelialization time decreased from 18.23 days in controls, to 14.97 days for TF1,14.77 days for TF-2, 17.55 days for OAQMC, 15.97 days for OAQ (MC+PN), 17.75 days for OAQPG, 16.24 days for OAQ (PG+PN), 15.81 days for OAQ (MC+PG+PN), 14.08 days for O3TF1, 13.79 days for O3TF2 and 18.88 days for GXII, while standard povidone iodine showed the lowest time of 15.50 days.The overall epithelialization time can be presented as: GXII O3TF2. Among the prepared herbal formulations, the blended formulation showed the decrease in epithelization period in comparison with controls, individual extracts and showed comparable results to the standard ointment…”

“…Burn wounds were created on dorsal part of shaved rat's skin surface using concentrate sulfuric acid, exposed for 10 s. After 24 h, dead tissues were excised using sterile surgical blade through a template designed to produce a third degree burn 14 . All groups were treated same as in excision model.…”

Effect of Hydrogen Sulfide on the Mitogen-Activated Protein Kinase Signaling Pathway in Cultured Skin Macrophages of Burned Rats

“…Sections (5 mm) were cut and stained with hematoxylin and eosin. The deep II burn (called burn in the following text), which was characterized as a partial deep burn damaging the upper area of the deep reticular dermis and the superficial papillary dermis, 11,12 was confirmed by the pathologic sections ( Fig. 1).…”