A new approach based on isoindole formation reaction for sensitive fluorimetric assay of milnacipran in tablets and biological fluids (plasma/urine)

Abstract

The current study describes a new, sensitive, and economic protocol for milnacipran analysis.

Green innovative fluorescence approach for the feasible and reliable assay of thiol-containing drugs; captopril as a model

“… 54 Therefore, the chain should be of reasonable length. In our laboratory, various compounds that contain aliphatic primary amino group such as gabapentin, 48 3-isobutyl gamma aminobutyric acid, 49 milnacipran, 50 and AMH 51 were analyzed through the isoindole formation reaction. However, the use of AMH was found to fulfil the aforementioned criteria of being has a side chain of appropriate length (C6) with two points of branching, one of them is close to the amino group.…”

“…The interaction of OPA with thiols, specially 2-ME and compounds containing primary amino group, was extensively used to determine primary amine compounds, [47][48][49][50][51] including pharmaceutical compounds. 52,53 Because of the essential presence of the thiol compound in the reaction vessel, the reaction could be possibly employed for the thiol-containing compound.…”

A new application of isoindole fluorophore derivative in sitagliptin antidiabetic medication assay: Application to dosage forms and biological fluid evaluation

“…Also, compared to an approach that relies on inherent fluorescence monitoring [40], this strategy's LOQ is more sensitive. When 2-ME is present, the functional amino group in STG allows it to condense with OPA to produce the fluorescent derivative isoindole [36][37][38][39]. Figure 1 shows the isoindole fluorescence product monitored at excitation (339.7 nm) and emission (434.6 nm) wavelengths, and Scheme 1 shows the pathway for derivative generation.…”

“…It is common practice to use OPA in conjunction with a thiol molecule, such as 2-ME, to derivatize amino compounds into luminous compounds. A number of medicinal substances have been quantified using this method [36][37][38][39]. Previously developed fluorometric methods for STG had disadvantages such as low sensitivity [14,15] or drastic coupling conditions with high-cost fluorogenic reagents [13].…”

An integrative analytical approach designed for feasible tranexamic acid assay using o‐phthalaldehyde as a fluorogenic probe: applications to tablets, ampoules, and urine

“…Therefore, we sought to use a different fluorimetric method that eliminates the disadvantages mentioned earlier. We chose the spectrofluorimetric method because it is inexpensive, is widely available, and requires no extraction or heating steps for o ‐phthalaldehyde condensation [25, 26]. The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines were used to validate the technique.…”