A new approach for obtaining trans-resveratrol from tree peony seed oil extracted residues using ionic liquid-based enzymatic hydrolysis in situ extraction

Chen, Fengli; Zhang, Xinglong; Du, Xiaoqiang; Yang, Lei; Zu, Yuangang; Yang, Fengchun

doi:10.1016/j.seppur.2016.06.056

Cited by 41 publications

(22 citation statements)

References 47 publications

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“…The mobile phase was degassed before use, which consisted of (A) ultrapure water containing 0.05% acetic acid and (B) HPLC-grade acetonitrile. Isocratic elution at A-B (78: 22) was carried out at a flow rate of 0.9 mL/min at room tem-perature for 12 min, followed by column wash at 40% B for 2 min before the next injection. The column temperature was 30 • C. The volume for sample injection was 10 uL and the chromatogram was recorded at 306 nm for t-RVT.…”

Section: Determination Of Trans-resveratrol By Hplcmentioning

confidence: 99%

“…Chen et al. compared the effect of various enzymes for obtaining t‐RVT from tree peony seed oil extracted residues and found that cellulase was efficient for extraction of t‐RVT. However, the enzyme‐assisted method has not been optimized in the extraction of t‐RVT in peanut seeds.…”

Section: Introductionmentioning

confidence: 99%

“…For example, cellulase was a complex enzyme system containing β-glucosidase for hydrolyzing the β-glucosidic linkages in the t-RVT glycosides and other enzymes for degradation of cell walls. Chen et al [22] compared the effect of various enzymes for obtaining t-RVT from tree peony seed oil extracted residues and found that cellulase was efficient for extraction of t-RVT. However, the enzyme-assisted method has not been optimized in the extraction of t-RVT in peanut seeds.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of extraction of total trans‐resveratrol from peanut seeds and its determination by HPLC

Luo

Chen

et al. 2020

J of Separation Science

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Resveratrol, a stilbene phytoalexin in plants, is believed to benefit human health. In this study, an optimized enzyme‐assisted method was developed to extract the total content of trans‐resveratrol (free or combined with glucose) in peanut seeds, followed by detection using high‐performance liquid chromatography. The extraction process was optimized by Box–Behnken design and response surface methodology. The optimized enzyme concentration, digestion time, pH, and temperature were 3.02 g/L, 57.06 min, 5.88, and 51.05°C, respectively. Validation tests indicated that the experimental yield of trans‐resveratrol was 0.183 ± 0.007 µg/g with a relative standard deviation of 3.87% (n = 5) under the optimal condition, which was closely agreed with the predicted value (0.182 µg/g). The recoveries obtained from the spiked samples were varied from 89.4 to 103.9%. Therefore, this study will provide a useful method for quantification of total trans‐resveratrol in peanut seeds.

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Section: Determination Of Trans-resveratrol By Hplcmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization of extraction of total trans‐resveratrol from peanut seeds and its determination by HPLC

Luo

Chen

et al. 2020

J of Separation Science

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“…The reason may be that when the substrate concentration is higher, the diffusion of the enzymolysis product and the enzyme transfer are limited during the enzymolysis process, and the feedback inhibition of microenvironment is intense. [24] So, compared the selected experimental ratios, the solid-liquid ratio of 1:6 (g/mL) was appropriate.…”

Section: Effect Of the Solid-liquid Ratiomentioning

confidence: 99%

Surfactant-assisted enzymatic extraction of piperine from Piper nigrum L

Yang

et al. 2020

International Journal of Food Properties

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Surfactant-assisted enzymatic hydrolysis was used to extract the flavor compounds from piper nigrum L. The extraction conditions were optimized through orthogonal and single-factor experiments. The extracted piperine content, measured with High-Performance Liquid Chromatography (HPLC) and UV spectrophotometric, was used as experimental index. The optimized conditions were as follows: the amount of cellulase, neutral protease and surfactant (sodium stearoyl lactylate) addition was 0.08%, 0.1% and 0.4% (w/w) respectively. The solid-liquid ratio was 1:5 (g/mL), the enzymolysis temperature and time were 60°C and 4 h, and the granularity was less than 10 mesh. Under these conditions, the content of the extracted piperine was 4.54% (UV spectrophotometric) and 4.42% (HPLC), respectively. Meanwhile, the mechanisms of the enzymatic reaction, as well as the role of the surfactant in enzymatic hydrolysis process were discussed.

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“…Nevertheless, severe water deficiency stress can limit the cultivation area, lead to smaller leaves and flowers, inhibit the synthesis of organic substances and flower pigments, and reduce the ornamental value and seed yield of tree peony (Li et al., ). Recent studies, however, have mainly focused on oil extraction (Chen et al., , b; Cui et al., ; Han et al., ). In addition, efficient protocols for the micropropagation of tree peony and the effects of different medium compositions and exogenous hormones on the browning of tree peony callus in tissue culture were recently investigated (Wen et al., ; Zhou et al., ).…”

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confidence: 99%

Tolerance strategies revealed in tree peony (Paeonia suffruticosa; Paeoniaceae) ecotypes differentially adapted to desiccation

Guo

Yin

et al. 2018

Appl Plant Sci

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Premise of the StudyTree peony (Paeonia suffruticosa; Paeoniaceae) is well known for its ornamental value, edible oil, and medicinal properties. However, its growing area has been limited by drought that has been exacerbated by global climate change.MethodsGene expression profiles of a drought‐tolerant cultivar and a drought‐sensitive cultivar during dehydration and rehydration were investigated by transcriptome analysis. Expression patterns of unigenes related to drought and recovery response and unrelated to either cultivar were classified by hierarchical clustering and real‐time quantitative PCR (qPCR).ResultsA total of 81,725 unigenes with a mean length of 762 nucleotides that may play roles in drought response were identified. Unigenes were characterized as being involved in lipid transport metabolism, proline metabolism, and photosynthesis. In addition, plant hormone signaling pathway genes were also characterized as potentially being involved in drought response. Expression patterns of the 20 drought‐responsive unigenes verified by qPCR showed a differential expression pattern under either the drought or recovery treatment.DiscussionThis is the first report to identify and verify unigenes of tree peonies with differing water sensitivity during dehydration and rehydration. This study offers a valuable resource for candidate genes involved in drought and provides insight into the breeding of drought‐resistant tree peony cultivars.

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A new approach for obtaining trans-resveratrol from tree peony seed oil extracted residues using ionic liquid-based enzymatic hydrolysis in situ extraction

Cited by 41 publications

References 47 publications

Optimization of extraction of total trans‐resveratrol from peanut seeds and its determination by HPLC

Optimization of extraction of total trans‐resveratrol from peanut seeds and its determination by HPLC

Surfactant-assisted enzymatic extraction of piperine from Piper nigrum L

Tolerance strategies revealed in tree peony (Paeonia suffruticosa; Paeoniaceae) ecotypes differentially adapted to desiccation

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