A new approach for sample preparation of protein hydrolyzates for amino acid analysis

Spitz, Harvey D.

doi:10.1016/0003-2697(73)90170-x

Cited by 80 publications

(31 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HCl was evaporated through a rotary evaporator while the residue was dissolved in 5 mL methanol. The essential amino acids were read directly using an amino acid analyzer (Model 126AA, Beckman, Fullerton, CA; Spitz, 1973;Moore, 1963). The essential amino acids determined were: arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.…”

Section: Methodsmentioning

confidence: 99%

Responses of Fruit Yield and Quality ofTrichosanthes cucumerinaLandraces to Phosphorus Rate

Adebooye

Oloyede

2006

Journal of Vegetable Science

View full text Add to dashboard Cite

Snake Gourd (Trichosanthes cucumerina L.), a member of the Cucurbitaceae, also locally known as Snake Tomato, has qualities that could make it a potential substitute for the solanaceous tomato [Lycopersicon esculentum (L.) Mill.], especially when the latter is not available during the year. However, there is little known about the mineral nutrition requirements of Snake Gourd, and how nutrition affects fruit yield and quality. Studies were conducted from University, Ile-Ife, Nigeria to evaluate the effect of phosphorus (P) on fruit yield and chemical composition of two landrace morphotypes of Snake Tomato. Five P treatment applications (0, 30, 60, 90, and 120 kgиha -1 of P 2 O 5 ) were evaluated using single super phosphate fertilizer (9% P). Morphotype had little effect on results. Yield increased as P level increased. Except for crude protein content, moisture content, and oxalate levels, which were not significantly affected by P treatment rates, nutrients, and essential amino acids compositions increased with increasing P treatment level. The high nutrient level and low oxalate composition indicate, that this vegetable can be used in the human diet. These results may help preserve biodiversity and increase the gene bank of neglected wild species of high-quality nutrient sources.

show abstract

Section: Methodsmentioning

confidence: 99%

Responses of Fruit Yield and Quality ofTrichosanthes cucumerinaLandraces to Phosphorus Rate

Adebooye

Oloyede

2006

Journal of Vegetable Science

View full text Add to dashboard Cite

show abstract

“…Milk samples were centrifuged (1558 ½ g, 20 min, 6°C) and skim milk was isolated. Preparation and analysis of skim milk samples for amino acids were conducted according to Spitz (1973), Gustavsson & Betner (1990), respectively. The analysed amino acids in milk were the same as for plasma, except for Met, Gln and Tyr.…”

Section: Plasma and Milk Analysesmentioning

confidence: 99%

Catheterization of Arteria Epigastrica Cranialis, Measurement of Nutrient Arteriovenous Differences and Evaluation of Daily Plasma Flow Across the Mammary Gland of Lactating Sows

Nielsen¹,

Pierzynowski²,

Børsting³

et al. 2002

Acta Agriculturae Scandinavica, Section A — Animal Science

View full text Add to dashboard Cite

Catheterization of arteria epigastrica cranialis, measurement of nutrient arteriovenous differences and evaluation of daily plasma ow across the mammary gland of lactating sows.The objectives of the present study were: (i) to evaluate a novel procedure for catheterization of the mammary artery; (ii) to measure amino acid, glucose and calcium arteriovenous differences in lactating sows; and (iii) to evaluate the use of different markers in estimating mammary plasma ow by the Fick principle. Five multiparous sows were tted with two catheters, in the cranial epigastric artery and anterior mammary vein. Catheterization of the cranial epigastric artery was carried out with a new surgical technique. Implanted catheters remained functional until day 15 (in two animals), day 21 (one animal) and day 27 (one animal), whereas in one case this technique failed. Sampling of arterial and venous blood took place on days 9, 15, 21 and 27 of lactation. Mean extraction rates 9 SEM (percent) were 31.2 9 3.1 arginine (Arg), 16.4 92.5 glutamine (Gln), 27.2 9 5.7 threonine (Thr), 8.3 9 2.6 glycine (Gly), 12.4 9 2.7 alanine (Ala), 20.2 9 3.4 tyrosine (Tyr), 18.1 92.8 proline (Pro), 28.2 96.2 methionine (Met), 18.592.2 valine (Val), 26.4 9 3.1 phenylalanine (Phe), 30.2 9 3.1 isoleucine (Ile), 41.4 9 3.4 leucine (Leu), 32.2 9 4.0 lysine (Lys) and 20.09 2.76 glucose. There was a relatively high abundance of lysine in milk compared with the amount extracted from plasma. This suggests lysine to be limiting in the present experimental situation, which was probably due to a low feed intake only providing 31.4 g Lys day ¼ 1 on average.

show abstract

“…The analysis was carried out in the Biorad analyser, adding buffer pH 2.2 to the hydrolysate (Spitz 1973). 2-Aminobutyric acid was used as internal standard (Roth 1976).…”

Section: Amino Acidsmentioning

confidence: 99%

Carob bean germ seed (Ceratonia siliqua): Study of the oil and proteins

Maza¹,

Zamora²,

Alaiz³

et al. 1989

J Sci Food Agric

View full text Add to dashboard Cite

The oil and flour of the seed germ of carob bean (Ceratonia siliqua L ) were studied. The fatty acid composition included, as main components, palmitic (14.2 %), stearic (3.0 %), oleic (38.5 %) and linoleic (43-6 %) acids. Lipolysis with pancreatic lipase shows the following glyceride composition: S 3 (0-0 %), U S 2 (2.7%), SU2 (27.9 %) and U 3 (63.7%). The protein pour was separated into protein classes according to their solubility and molecular weight, and the preparation of an isolate (with more than 90 % protein) is described.

show abstract

A new approach for sample preparation of protein hydrolyzates for amino acid analysis

Cited by 80 publications

References 4 publications

Responses of Fruit Yield and Quality ofTrichosanthes cucumerinaLandraces to Phosphorus Rate

Responses of Fruit Yield and Quality ofTrichosanthes cucumerinaLandraces to Phosphorus Rate

Catheterization of Arteria Epigastrica Cranialis, Measurement of Nutrient Arteriovenous Differences and Evaluation of Daily Plasma Flow Across the Mammary Gland of Lactating Sows

Carob bean germ seed (Ceratonia siliqua): Study of the oil and proteins

Contact Info

Product

Resources

About