A new approach for the analysis of amino acid neurotransmitters in mouse brain tissues using DESI imaging

Maciel, Lanaia Ítala Louzeiro; Vaz, Boniek Gontijo; Ramalho, Ruver Rodrigues Feitosa; Ribeiro, Raul Izidoro; Pinto, Mauro Cunha Xavier; Vaz, Boniek G.

doi:10.1016/j.ijms.2021.116730

Cited by 6 publications

(6 citation statements)

References 39 publications

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“…DESI was performed using an Omni Spray Ion Source 2D (Prosolia Inc.) coupled to a Thermo Scientific Q-Exactive Hybrid Quadrupole-Orbitrap . The system is illustrated in Figure S1.…”

Section: Methodsmentioning

confidence: 99%

“…DESI was performed using an Omni Spray Ion Source 2D (Prosolia Inc.) coupled to a Thermo Scientific Q-Exactive Hybrid Quadrupole-Orbitrap. 37 The system is illustrated in Figure S1. In detail, the rocks were mounted on the 2D moving stage, and their surfaces were scanned under impinging charged droplets using 2 μL/min MeOH, MeOH/ Tol (7:3), MeOH/Tol (6:4), or MeOH/Tol (5:5) as a spray solvent.…”

Section: ■ Introductionmentioning

confidence: 99%

“…14,41 Another interesting finding was the detection of m/z 377, identified as a chlorinated disaccharide. Its isotopic profile showed a peak at m/z 379 corresponding to [M + 37 Cl] − , and its MS/MS spectrum (Figure S7) showed the loss of chlorine and a proton to form the deprotonated disaccharide at m/z 341 and then the loss of a monosaccharide to generate the m/z 179. 42 Aquatic plants or bacteria may have produced this sugar.…”

Section: ■ Introductionmentioning

confidence: 99%

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Directly Mapping the Spatial Distribution of Organic Compounds on Mineral Rock Surfaces by DESI and LAESI Mass Spectrometry Imaging

et al. 2022

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Here, we present a new application of desorption electrospray ionization (DESI) and laser ablation electrospray ionization (LAESI) mass spectrometry imaging to assess the spatial location of organic compounds, both polar and nonpolar, directly from rock surfaces. Three carbonaceous rocks collected from an aquatic environment and a berea sandstone subjected to a small-scale oil recovery experiment were analyzed by DESI and LAESI. No rock pretreatment was required before DESI and LAESI analyses. DESI detected and spatially mapped several fatty acids and a disaccharide on the surfaces of carbonaceous rocks, and various nitrogenated and oxygenated compounds on the surfaces of berea sandstone. In contrast, LAESI using a 3.4 μm infrared laser beam was able to detect and map hydrocarbons on the surfaces of all rock samples. Both techniques can be combined to analyze polar and nonpolar compounds. DESI can be used first to detect polar compounds, as it does not destroy the rock surface, and LAESI can then be used to analyze nonpolar analytes, as it destroys a layer of the sample surface. Both techniques have the potential to be used in several scientific areas involving rocks and minerals, such as in the analysis of industry-derived contaminants in aquatic sediments or in small-scale rock–fluid interaction experiments.

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“…DESI was performed using an Omni Spray Ion Source 2D (Prosolia Inc.) coupled to a Thermo Scientific Q-Exactive Hybrid Quadrupole-Orbitrap . The system is illustrated in Figure S1.…”

Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Directly Mapping the Spatial Distribution of Organic Compounds on Mineral Rock Surfaces by DESI and LAESI Mass Spectrometry Imaging

et al. 2022

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“…Ambient ionization techniques, such as desorption electrospray ionization (DESI) and its variants, are mostly employed for the real-time analysis of solids or viscous liquids using MS. , Such ionization strategies can facilitate MS imaging and monitor the ensuing chemical reactions in situ . − For the direct analysis of solid surfaces, the limit of detection (LOD) is a crucial parameter, and several attempts have been made to optimize it, with an ultimate goal of enhancing the signal intensity and/or expanding the analyte area. A prominent way to achieve this objective involves the chemical derivatization of the surface or a specific pretreatment, leading to sample desorption. − For example, during the vibrating sharp-edge spray ionization process, liquid samples undergo desorption aided by a vibrating glass slide.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Sniffing Mass Spectrometry Aided by Venturi Self-Pumping Applicable to Gaseous and Solid Surface Analysis

Chen

et al. 2022

Anal. Chem.

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Based on the Venturi self-pumping effect, real-time sniffing with mass spectrometry (R-sniffing MS) is developed as a tool for direct and real-time mass spectrometric analysis of both gaseous and solid samples. It is capable of dual-mode operation in either gaseous or solid phase, with the corresponding techniques termed as Rg-sniffing MS and Rs-sniffing MS, respectively. In its gaseous mode, Rg-sniffing MS is capable of analyzing a gaseous mixture with response time (0.8–2.1 s rise time and 7.3–9.6 s fall time), spatial resolution (<80 μm), three-dimensional diffusion imaging, and aroma distribution imaging of red pepper. In its solid mode, an appropriate solvent droplet desorbs the sample from a solid surface, followed by the aspiration of the mixture using the Venturi self-pumping effect into the mass spectrometer, wherein it is ionized by a standard ion source. Compared with the desorption electrospray ionization (DESI) technique, Rs-sniffing MS demonstrated considerably improved limit of detection (LOD) values for arginine (0.07 μg/cm2 Rs-sniffing vs 1.47 μg/cm2 DESI), thymopentin (0.10 μg/cm2 vs 2.67 μg/cm2), and bacitracin (0.16 μg/cm2 vs 2.28 μg/cm2). Rs-sniffing is applicable for the detection of C60(OCH3)6Cl–, an intermediate in the methoxylation reaction involving C60Cl6 (solid) and methanol (liquid). The convenient and highly sensitive R-sniffing MS has a characteristic separation of desorption from the ionization process, in which the matrix atmosphere of desorption can be interfaced by a pipe channel and self-pumped by the Venturi effect with consequent integration using a standard ion source. The R-sniffing MS operates in a voltage-, heat-, and vibration-free environment, wherein the analyte is ionized by a standard ion source. Consequently, a wide range of samples can be analyzed simultaneously by the R-sniffing MS technique, regardless of their physical state.

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“…With the aid of a variety of ionization sources operated under vacuum or ambient atmospheric condition (e.g. MALDI, SIMS, DESI, LA-ESI), MSI technique has been successfully applied to detect synthetic drugs, metabolites, lipids, peptides, proteins, metals, glycans, etc 7,8,9,10 . A multiplex molecular changes caused by disease progression or drug intervention can be visualized at the whole-body, organ, tissue, cell or even sub-cellular resolution 11,12,13,14 .…”

Section: Introductionmentioning

confidence: 99%

X-dimensional Mass Spectrometry Imaging Discovers Spatially Resolved Metabolic Response

Song

Zang

Zhang

et al. 2022

Preprint

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This study developed an open-source method, x-dimensional mass spectrometry imaging (MSI(x)), to reveal the spatially-resolved high-order metabolomics information associated with disease progression or drug action. This high order bioinformation includes metabolism pathway, species, biofunction, or biotransformation, which involves multiplex metabolites and cannot be presented by a single ion image. The MSI(x) enables the evaluation of the physiological status, the discovery of therapeutic or adverse effects, the investigation of regional heterogeneous response to drug treatment, possible molecular mechanism, and even drug potential target. MSI(x) was demonstrated to be a promising molecular imaging tool not only for efficacy and safety evaluation but also for the molecular mechanism investigation at the early stage of drug research and development.

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A new approach for the analysis of amino acid neurotransmitters in mouse brain tissues using DESI imaging

Cited by 6 publications

References 39 publications

Directly Mapping the Spatial Distribution of Organic Compounds on Mineral Rock Surfaces by DESI and LAESI Mass Spectrometry Imaging

Directly Mapping the Spatial Distribution of Organic Compounds on Mineral Rock Surfaces by DESI and LAESI Mass Spectrometry Imaging

Real-Time Sniffing Mass Spectrometry Aided by Venturi Self-Pumping Applicable to Gaseous and Solid Surface Analysis

X-dimensional Mass Spectrometry Imaging Discovers Spatially Resolved Metabolic Response

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