A new approach to determine the susceptibility of bacteria to antibiotics directly from positive blood culture bottles in two hours

March, Gabriel A.; Garcia-Loygorri, Maria C.; Simarro, Marı́a; Gutiérrez, María P.; Orduña, Antonio; Bratos, Miguel Ángel

doi:10.1016/j.mimet.2014.12.007

Cited by 16 publications

(10 citation statements)

References 34 publications

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“…The viable bacteria percentage obtained from the cells incubated with meropenem were compared, using ROC curves, to the viable bacterial percentage from paired antibiotic‐free control. It was evaluated that the viable bacterial percentage obtained from K. pneumoniae after 1 hr of incubation made it possible to define whether a K. pneumoniae isolate was resistant or susceptible to meropenem . This incubation time is similar to other susceptibility studies performed in Enterobacteriaceae .…”

Section: Discussionmentioning

confidence: 99%

“…It was evaluated that the viable bacterial percentage obtained from K. pneumoniae after 1 hr of incubation made it possible to define whether a K. pneumoniae isolate was resistant or susceptible to meropenem . This incubation time is similar to other susceptibility studies performed in Enterobacteriaceae . Based on the analysis of the ROC curves, the best validity and sensitivity data were obtained with a cut‐off value of 18.88%.…”

Section: Discussionmentioning

confidence: 99%

“…FCM is a rapid, powerful high‐throughput technology for the differentiation and functional analysis of microorganisms. FCM has also been used previously for antibiotic‐susceptibility testing of several clinical important microorganisms .…”

Section: Introductionmentioning

confidence: 99%

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Rapid Identification of Klebsiella pneumoniae by Matrix‐Assisted Laser Desorption/Ionization‐Time of Flight Mass Spectrometry and Detection of Meropenem Resistance by Flow Cytometric Assay

Kılıç

Dogan

Kaya

et al. 2016

Clinical Laboratory Analysis

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Rapid Identification of Klebsiella pneumoniae by Matrix‐Assisted Laser Desorption/Ionization‐Time of Flight Mass Spectrometry and Detection of Meropenem Resistance by Flow Cytometric Assay

Kılıç

Dogan

Kaya

et al. 2016

Clinical Laboratory Analysis

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“…Isolate testing by proteomics has been reported to be faster and less costly for many of these infections [134–136]. Moreover, a single test can determine not only the bacterial strain but also antibiotic resistance mechanisms reliant upon alterations in protein expression [111,137,138]. …”

Section: Personalized Proteomicsmentioning

confidence: 99%

Personalized Proteomics: The Future of Precision Medicine

2016

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Medical diagnostics and treatment has advanced from a one size fits all science to treatment of the patient as a unique individual. Currently, this is limited solely to genetic analysis. However, epigenetic, transcriptional, proteomic, posttranslational modifications, metabolic, and environmental factors influence a patient’s response to disease and treatment. As more analytical and diagnostic techniques are incorporated into medical practice, the personalized medicine initiative transitions to precision medicine giving a holistic view of the patient’s condition. The high accuracy and sensitivity of mass spectrometric analysis of proteomes is well suited for the incorporation of proteomics into precision medicine. This review begins with an overview of the advance to precision medicine and the current state of the art in technology and instrumentation for mass spectrometry analysis. Thereafter, it focuses on the benefits and potential uses for personalized proteomic analysis in the diagnostic and treatment of individual patients. In conclusion, it calls for a synthesis between basic science and clinical researchers with practicing clinicians to design proteomic studies to generate meaningful and applicable translational medicine. As clinical proteomics is just beginning to come out of its infancy, this overview is provided for the new initiate.

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“…The essential agreement of the test with the commercial methods was 99%. 162 Numerous further potential AST configurations are under development. These near or more distant future technologies have been reviewed by other authors.…”

Section: Endimiani and Jacobsmentioning

confidence: 99%

The Changing Role of the Clinical Microbiology Laboratory in Defining Resistance in Gram-negatives

Endimiani

Jacobs

2016

Infectious Disease Clinics of North America

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A new approach to determine the susceptibility of bacteria to antibiotics directly from positive blood culture bottles in two hours

Cited by 16 publications

References 34 publications

Rapid Identification of Klebsiella pneumoniae by Matrix‐Assisted Laser Desorption/Ionization‐Time of Flight Mass Spectrometry and Detection of Meropenem Resistance by Flow Cytometric Assay

Rapid Identification of Klebsiella pneumoniae by Matrix‐Assisted Laser Desorption/Ionization‐Time of Flight Mass Spectrometry and Detection of Meropenem Resistance by Flow Cytometric Assay

Personalized Proteomics: The Future of Precision Medicine

The Changing Role of the Clinical Microbiology Laboratory in Defining Resistance in Gram-negatives

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