A new approach to finding optimal centrifugation conditions for shear-sensitive microalgae

Molina-Miras, A.; López-Rosales, L.; Cerón-García, M.C.; Sánchez-Mirón, A.; Camacho, F. Garcı́a; Contreras-Gómez, Antonio; Molina-Grima, E.

doi:10.1016/j.algal.2019.101677

Cited by 15 publications

(10 citation statements)

References 30 publications

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“…The first of them would correspond to a possible breakage of cells by excess centrifugation treatment and the release of intracellular AMs to the supernatant. Although it cannot be dismissed, it is unlikely that lysis was as significant, as centrifugation conditions to prevent it were selected on the basis of a previous study [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Isolation and Structural Elucidation of New Amphidinol Analogues from Amphidinium carterae Cultivated in a Pilot-Scale Photobioreactor

et al. 2021

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The demand for valuable products from dinoflagellate biotechnology has increased remarkably in recent years due to their many prospective applications. However, there remain many challenges that need to be addressed in order to make dinoflagellate bioactives a commercial reality. In this article, we describe the technical feasibility of producing and recovering amphidinol analogues (AMs) excreted into a culture broth of Amphidinium carterae ACRN03, successfully cultured in an LED-illuminated pilot-scale (80 L) bubble column photobioreactor operated in fed-batch mode with a pulse feeding strategy. We report on the isolation of new structurally related AMs, amphidinol 24 (1, AM24), amphidinol 25 (2, AM25) and amphidinol 26 (3, AM26), from a singular fraction resulting from the downstream processing. Their planar structures were elucidated by extensive NMR and HRMS analysis, whereas the relative configuration of the C-32®C-47 bis-tetrahydropyran core was confirmed to be antipodal in accord with the recently revised configuration of AM3. The hemolytic activities of the new metabolites and other related derivatives were evaluated, and structure–activity conclusions were established. Their isolation was based on a straightforward and high-performance bioprocess that could be suitable for the commercial development of AMs or other high-value compounds from shear sensitive dinoflagellates.

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Section: Resultsmentioning

confidence: 99%

Isolation and Structural Elucidation of New Amphidinol Analogues from Amphidinium carterae Cultivated in a Pilot-Scale Photobioreactor

et al. 2021

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“…Once the stationary phase of strains A01BR, ACRN02, ACRN03, DN241EHU, and CCMP1314 was reached, the cultures were deposited in 50 mL Falcon tubes and centrifuged at 2500× g for 5 min at room temperature in a benchtop centrifuge (Beckman Coulter, model Allegra 25R, Madrid, Spain) using a rotor (swing-out head) with a maximum radius of 13.7 cm (max RCF = 15,300× g ). These centrifugation conditions were within the operating window previously determined for Amphidinium carterae where maximum cell recovery and cell separation-cell integrity are guaranteed [ 33 ]. The pellets were gently washed with distilled water.…”

Section: Methodsmentioning

confidence: 99%

LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

et al. 2020

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Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.

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“…Despite the fact that centrifugation is widely used for research and small-scale operations due to its high reliability and efficiency, Molina-Miras et al [61] and Xu et al [62] have proven in their studies that damage to microalgae cells occurs because of the high shear rates and high centrifugal forces between the cells during the harvesting process. The conditions needed to reach complete microalgae cell separation from biomass tend to damage the cells [12]. The cell damage occurs in relation to the hydro dynamic shear forces, the velocity gradients, relative cell-fluid movement during the settlement, and the compressive centrifugal forces to which microalgae cells are subjected in the pellet [12].…”

Section: Suspended Microalgal Cultivationmentioning

confidence: 99%

“…The conditions needed to reach complete microalgae cell separation from biomass tend to damage the cells [12]. The cell damage occurs in relation to the hydro dynamic shear forces, the velocity gradients, relative cell-fluid movement during the settlement, and the compressive centrifugal forces to which microalgae cells are subjected in the pellet [12]. Therefore, two significance parameters that determine the cell survival in the centrifugation process are how long the microalgae cells stay in the pellet and the G-force applied in the process [12].…”

Section: Suspended Microalgal Cultivationmentioning

confidence: 99%

“…However, the microalgal biomass harvesting methods for the suspended microalgal cultivation require a high amount of energy to separate the water medium from mature cells before proceeding to the biofuel procedures [4]. Furthermore, the methods that are often used for microalgae harvesting such as centrifugation and flocculants not only require more energy to perform but also lead to cell damage [12] and contamination of the harvested biomass [13]. This is where attached microalgal cultivation becomes the solution for all the issues associated with suspended cultivation to produce third-generation biofuels.…”

Section: Introductionmentioning

confidence: 99%

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Assuaging Microalgal Harvesting Woes via Attached Growth: A Critical Review to Produce Sustainable Microalgal Feedstock

et al. 2021

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Third-generation biofuels that are derived from microalgal biomass have gained momentum as a way forward in the sustainable production of biodiesel. Such efforts are propelled by the intention to reduce our dependence on fossil fuels as the primary source of energy. Accordingly, growing microalgal biomass in the form of suspended cultivation has been a conventional technique for the past few decades. To overcome the inevitable harvesting shortcomings arising from the excessive energy and time needed to separate the planktonic microalgal cells from water medium, researchers have started to explore attached microalgal cultivation systems. This cultivation mode permits the ease of harvesting mature microalgal biomass, circumventing the need to employ complex harvesting techniques to single out the cells, and is economically attractive. However, the main bottleneck associated with attached microalgal growth is low biomass production due to the difficulties the microalgal cells have in forming attachment and populating thereafter. In this regard, the current review encompasses the novel techniques adopted to promote attached microalgal growth. The physicochemical effects such as the pH of the culture medium, hydrophobicity, as well as the substratum surface properties and abiotic factors that can determine the fate of exponential growth of attached microalgal cells, are critically reviewed. This review aims to unveil the benefits of an attached microalgal cultivation system as a promising harvesting technique to produce sustainable biodiesel for lasting applications.

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A new approach to finding optimal centrifugation conditions for shear-sensitive microalgae

Cited by 15 publications

References 30 publications

Isolation and Structural Elucidation of New Amphidinol Analogues from Amphidinium carterae Cultivated in a Pilot-Scale Photobioreactor

Isolation and Structural Elucidation of New Amphidinol Analogues from Amphidinium carterae Cultivated in a Pilot-Scale Photobioreactor

LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

Assuaging Microalgal Harvesting Woes via Attached Growth: A Critical Review to Produce Sustainable Microalgal Feedstock

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