A new approach to the treatment of recurrent aphthous stomatitis with bioadhesive gels containing cyclosporine A solid lipid nanoparticles: in vivo/in vitro examinations

Abstract

Aim:To develop a suitable buccal bioadhesive gel formulation containing cyclosporine A solid lipid nanoparticles (CsA SLNs) for the treatment of recurrent aphthous stomatitis. Methods: The suitability of the prepared formulations for buccal application was assessed by means of rheological studies, textural profile analysis, and ex vivo drug-release studies. Plastic flows, typical gel-like spectra, and suitable mechanical properties were obtained from prepared formulations. The retention time was explored in in vivo distribution studies and the effect of the gel containing CsA SLNs on the healing of oral mucosal ulceration was investigated in an animal model. In vivo distribution studies are a very important indicator of the retention time of formulations at the application site. Results: Distribution studies showed that 64.76% ± 8.35% of the formulation coded "F8+SLN" remained on the buccal mucosa 6 hours after application. For the second part of the in vivo experiments, 36 rabbits were separated into three groups: the first group was treated with the gel formulation without the active agent; the second group with the gel formulation containing CsA SLNs; and the third group, used as the control group, received no treatment. Wound healing was established by scoring of the rate of wound healing on Days 3, 6, 9, and 12. Histological observations were made on the same days as the scoring studies. The bioadhesive gel formulation that included CsA SLNs increased the rate of mucosal repair significantly. Conclusion: This study has shown that the bioadhesive gel formulation containing CsA SLNs reported here is a promising candidate for the topical treatment of recurrent aphthous stomatitis.

Evaluation of chitosan based vaginal bioadhesive gel formulations for antifungal drugs

“…In our study, we evaluated vaginal retention using a new imaging method: Odyssey Infrared Imaging System with MousePOD™ adapter (LI-COR Biosciences). The benefit of this method is that live animals are used and that they are not exposed to radiation like in radio-imaging studies (34). Near-infrared images of rats administered RDye 800RS Carboxylate dye (peak absorption at 786 nm) loaded chitosan gels are shown in Fig.…”

Utilização de nanopartículas no tratamento de feridas: revisão sistemática

“…In study A7, when compared to the control group, the use of Ciclosporin A gel with solid lipid nanoparticles (NLS/CsA-loaded) showed a reduction in the oral wound area and significantly increased the rate of mucosal repair (22) . The increase in healing speed was also analyzed in study A8, in which a nitric oxide nanoparticle (NO-NP) dressing was used.…”

“…A smaller wound area was observed in the poly-Ag group than in the untreated groups and in the Staphylococcus group on days four and seven. A7 Karavana et al 2012 (22) A new approach to the treatment of recurrent aphthous stomatitis with bioadhesive gels containing cyclosporine A solid lipid nanoparticles: in vivo/in vitro examinations A gel formulation of Cyclosporine A loaded with solid lipid nanoparticles (NLS/CsA-loaded) was developed and applied in vivo. The observations were made in rabbits, divided into three groups, in which gingival ulcers were made for application and analysis of the product.…”

Development and evaluation of coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced dermal delivery

“…It has been shown that systemic uptake of active substances (e.g., isotretinoin) through skins could be avoided and SLN could increase the accumulative uptake of the active material in the skin (15). SLNs are produced as liquid dispersions and their incorporation into hydrogels or emulsions helps enhance the retention time in the applied area and patient compliance (16,17).Hydrogels are generally formed from carbomers that have the ability to absorb water, get hydrated and swell by neutralization with a base. Carbomers have been mainly used in liquid or semi-solid pharmaceutical formulations in order to modify their flow characteristics (17,18).…”

“…SLNs are produced as liquid dispersions and their incorporation into hydrogels or emulsions helps enhance the retention time in the applied area and patient compliance (16,17).…”