A new aspect of <i>in vitro</i> antimicrobial leukocyte- and platelet-rich plasma activity based on flow cytometry assessment

Cieślik-Bielecka, A.; Reichert, Paweł; Skowroński, Rafał; Królikowska, Aleksandra; Bielecki, Tomasz

doi:10.1080/09537104.2018.1513472

Cited by 13 publications

(12 citation statements)

References 37 publications

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“…The increased leukocyte counts within H-PRF were hypothesized to dramatically enhance the antimicrobial properties of PRF compared with those achieved with traditional L-PRF protocols. This finding was also supported by Cieślik-Bielecka et al 32 .…”

Section: Discussionsupporting

confidence: 79%

Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation

et al. 2020

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Platelet-rich fibrin (PRF) has been widely used owing to its ability to stimulate tissue regeneration. To date, few studies have described the antibacterial properties of PRF. Previously, PRF prepared by horizontal centrifugation (H-PRF) was shown to contain more immune cells than leukocyte- and platelet-rich fibrin (L-PRF). This study aimed to compare the antimicrobial effects of PRFs against Staphylococcus aureus and Escherichia coli in vitro and to determine whether the antibacterial effects correlated with the number of immune cells. Blood samples were obtained from eight healthy donors to prepare L-PRF and H-PRF. The sizes and weights of L-PRF and H-PRF were first evaluated, and their antibacterial effects against S. aureus and E. coli were then tested in vitro using the inhibition ring and plate-counting test methods. Flow-cytometric analysis of the cell components of L-PRF and H-PRF was also performed. No significant differences in size or weight were observed between the L-PRF and H-PRF groups. The H-PRF group contained more leukocytes than the L-PRF group. While both PRFs had notable antimicrobial activity against S. aureus and E. coli, H-PRF demonstrated a significantly better antibacterial effect than L-PRF. Furthermore, the antimicrobial ability of the PRF solid was less efficient than that of wet PRF. In conclusion, H-PRF exhibited better antibacterial activity than L-PRF, which might have been attributed to having more immune cells.

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Section: Discussionsupporting

confidence: 79%

Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation

et al. 2020

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“…According to the literature, the presence of leukocytes in PRP could affect the levels of important growth factors such as VEGF and EGF, and may also have antibacterial or immune-regulating effects. 17,18,30 While we did not observe a correlation between WBC and both VEGF and EGF, a positive correlation with PDGF-AA was found. This finding has not been reported in previous research, likely because PDGF-AA is not a frequently analyzed cytokine.…”

Section: Discussioncontrasting

confidence: 80%

Analysis and comparison of autologous platelet-rich plasma preparation systems used in the treatment of enthesopathies: A preliminary study

Dejnek¹,

Moreira²,

Płaczkowska³

et al. 2021

Adv Clin Exp Med

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“…Cieslik-Bielecka et al evaluated the antibacterial effect of L-PRP against selected bacterial strains (MRSA, MSSA, extended spectrum beta-lactamase, E. coli , K. pneumoniae , E. faecalis , and P. aeruginosa ) in vitro, and correlated antimicrobial effect with leukocyte and platelet counts (N = 20 healthy males) [ 46 ]. The result showed that L-PRP was activated using different concentrations of thrombin and calcium chloride, and the activated products were tested in following groups: G1, 20 μL of L-PRP and 5 μL of autologous thrombin (gelatinous mass); G2, 20 μL of L-PRP and 2 μL of autologous thrombin (gelatinous mass); G3, 25 μL of liquid L-PRP; G4, 25 μL of autologous thrombin; G5, 20 μL of L-PRP and 5 μl of bovine thrombin in a calcium chloride solution; G6, 20 μL of L-PRP and 2 μL of bovine thrombin in a calcium chloride solution; and G7, 25 μL of bovine thrombin in a calcium chloride solution.…”

Section: Bench Experimentsmentioning

confidence: 99%

“…There is weak preclinical evidence suggesting that when used as an adjunct to antibiotics, PRP may have a synergistic effect; however, other studies contradict this [ 3 , 46 ]. Platelet lysates showed a synergistic effect with β-lactam antibiotic (oxacillin) and glycopeptide (vancomycin) but not with oxazolidinone (linezolid) [ 31 ].…”

Section: Is There a Synergistic Effect Between Antibiotics And Prp On Bacterial Killing?mentioning

confidence: 99%

“…Similar observations were reported by other authors who found no correlation between antimicrobial activity and the concentration of platelets and leukocytes. In contrast, Cieslik-Bielecka et al suggested that a strong relationship was observed among selected leukocyte subtypes (T and B lymphocytic NK cells, monocytes, and granulocytes with CD45) with antimicrobial activity of L-PRP [ 46 , 60 ]. Other authors suggested that PRP significantly increased the proliferation and migration of fibroblasts, indicating a role of PRP in regeneration of damaged tissue [ 47 ].…”

Section: What Is the Mechanism Involved In The Antibacterial Effect Of Different Components Of Prp And Can It Be Enhanced?mentioning

confidence: 99%

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Systematic literature review evaluating evidence and mechanisms of action for platelet-rich plasma as an antibacterial agent

Sethi

Martin²,

Shrotriya³

et al. 2021

J Cardiothorac Surg

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Platelet rich plasma or PRP is a supraphysiologic concentrate of platelets derived by centrifugation and separation of whole blood components. Along with platelets and plasma, PRP contains various cell types including white blood cells (WBC)/leukocytes, both granulocytes (neutrophils, basophils, eosinophils) and agranulocytes (monocytes, lymphocytes). Researchers and clinicians have explored the application of PRP in wound healing and prevention of surgical wound infections, such as deep sternal wounds. We conducted this systematic literature review to evaluate the preclinical and clinical evidence for the antibacterial effect of PRP and its potential mechanism of action. 526 records were identified for screening. 34 unique articles were identified to be included in this literature review for data summary. Overall, the quality of the clinical trials in this review is low, and collectively qualify as Oxford level C. Based on the available clinical data, there is a clear trend towards safety of autologous PRP and potential efficacy in deep sternal wound management. The preclinical and bench data is very compelling. The application of PRP in treatment of wounds or prevention of infection with PRP is promising but there is a need for foundational bench and preclinical animal research to optimize PRP as an antibacterial agent, and to provide data to aid in the design and conduct of well-designed RCTs with adequate power to confirm antimicrobial efficacy of PRP in specific disease states and wound types.

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A new aspect of in vitro antimicrobial leukocyte- and platelet-rich plasma activity based on flow cytometry assessment

Cited by 13 publications

References 37 publications

Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation

Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation

Analysis and comparison of autologous platelet-rich plasma preparation systems used in the treatment of enthesopathies: A preliminary study

Systematic literature review evaluating evidence and mechanisms of action for platelet-rich plasma as an antibacterial agent

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