A new automated cell washer device for thawed cord blood units

Perotti, Cesare; Fante, Claudia Del; Viarengo, Gianluca; Papa, P.; Rocchi, Lorenzo; Bergamaschi, Paola; Bellotti, Laura; Marchesi, Andrea; Salvaneschi, L.

doi:10.1111/j.1537-2995.2004.03389.x

Cited by 43 publications

(39 citation statements)

References 28 publications

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“…Antonenas et al (2002) observed a loss of 27-30% of nucleated cells due to postthaw washing of umbilical cord blood. Perotti et al (2004) observed a similar loss. Laroche et al (2005) suggested that the wash step be omitted particularly in certain categories of patients where the loss of cells may have a detrimental effect.…”

Section: Introductionsupporting

confidence: 71%

Experimental study of diffusion-based extraction from a cell suspension

Mata

Longmire

McKenna

et al. 2008

Microfluid Nanofluid

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A recently proposed application of microfluidics is the post-thaw processing of biological cells. Numerical simulations suggest that diffusion-based extraction of the cryoprotective agent dimethyl sulfoxide (DMSO) from blood cells is viable and more efficient than centrifugation, the conventional method of DMSO removal. In order to validate the theoretical model used in these simulations, a prototype was built and the flow of two parallel streams, a suspension of Jurkat cells containing DMSO and a wash stream that contained neither cells nor DMSO, was characterized experimentally. DMSO transport in a rectangular channel (depth 500 lm, width 25 mm and overall length 125 mm) was studied as a function of three dimensionless parameters: depth ratio of the streams, cell volume fraction in the cell solution, and the Peclet number (Pe) based on channel depth, average flow rate and the diffusion coefficient for DMSO in water. In our studies, values of Pe ranged from O(10 3 ) to O(10 4 ). Laminar flow was ensured by keeping the Reynolds number between O(1) and O(10). Experimental results based on visual and quantitative data demonstrate conclusively that a microfluidic device can effectively remove DMSO from liquid and cell laden streams without compromising cell recovery. Also, flow conditions in the microfluidic device appear to have no adverse effect on cell viability at the outlet. Further, the results demonstrate that we can predict the amount of DMSO removed from a given device with the theoretical model mentioned previously.

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Section: Introductionsupporting

confidence: 71%

Experimental study of diffusion-based extraction from a cell suspension

Mata

Longmire

McKenna

et al. 2008

Microfluid Nanofluid

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“…Many in vitro studies have reported that the freezing phase can lower by as much as 30% the viability and functional capacity of the cryopreserved product [8][9][10][11]. In the present study, whose aim was to evaluate the manual method of thawing described by Rubinstein et al [8], we obtained a cell viability of approximately 73%.…”

Section: Discussionmentioning

confidence: 96%

Viability and functional capacity after thawing of hematopoietic progenitor cells cryopreserved at a cord blood stem cell bank in Colombia

Portillo

Guzmán

Rojas

et al. 2010

Intl J Gynecology & Obste

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“…Quantifying CD34+ cell content in frozen and thawed HSC products is also challenging for some of the same reasons described above. For example, studies have reported CD34+ cell recovery of >100% and as high as 200% [65,94]. There is no evi-dence that the freezing process spurs proliferation of HSCs.…”

Section: Post-thaw Assessmentmentioning

confidence: 99%

“…Losses of 27-30% of nucleated cells were observed by Antonenas and colleagues [61], with slightly lower losses observed in a subsequent study [64] using modified centrifugation technique to maximize cell recovery. Using an automated cell washer to remove DMSO from frozen and thawed cord blood resulted in similar losses [65]. Technologies have been developed for the removal of CPA solutions from frozen and thawed blood cells (not HSC products).…”

Section: Emerging Technologymentioning

confidence: 99%

Cryopreservation of Hematopoietic Stem Cells: Emerging Science, Technology and Issues

Fleming

Hubel

2007

Transfus Med Hemother

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Cell therapies based on hematopoietic stem cells (HSCs) have become the standard of care for a variety of diseases, and the number of patients and disorders being treated using HSCs continues to grow. Effective methods of preservation are critical for the clinical implementation of HSC-based therapies. Preservation permits the completion of safety and quality control testing, transportation to the site of use and coordination of the therapy with patient care regimes. This review discusses advances in the basic science of preservation that will permit us to improve preservation protocols for HSCs and other cell types. Recent advances in preservation technology can also be used to reduce cell losses during cell processing for preservation and improve the safety of HSCs used therapeutically. Finally, the growth of HSC therapy worldwide has created interest in alternative methods of preservation. This review also includes a discussion of different methods of freezing and storage. Improved methods for preserving HSCs will improve not only therapies based on these cells but can improve and speed development of preservation protocols for other cell types used therapeutically.

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A new automated cell washer device for thawed cord blood units

Cited by 43 publications

References 28 publications

Experimental study of diffusion-based extraction from a cell suspension

Experimental study of diffusion-based extraction from a cell suspension

Viability and functional capacity after thawing of hematopoietic progenitor cells cryopreserved at a cord blood stem cell bank in Colombia

Cryopreservation of Hematopoietic Stem Cells: Emerging Science, Technology and Issues

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