A new basic metachromatic dye, 1?9-Dimethyl Methylene Blue

Taylor, Kenneth B.; Jeffree, Grace M.

doi:10.1007/bf01081408

Cited by 73 publications

(24 citation statements)

References 2 publications

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“…DMB reagent (2.5 ml) was then added and the absorbance at 525 nM was read after 15 secs in a spectrophotometer. The DMB reagent was made according to the method published by Tayler and Jeffree (1969) and prepared by adding 3.04 g of glycine, 2.37 g of NaCl and 16 mg of 1.9-dimethylemethylene blue to one litre of deionised water. The pH was adjusted to 0.3 with HCI.…”

Section: Gag Assaymentioning

confidence: 99%

Glycosaminoglycans in horses with osteoarthritis

Alwan

Carter

Bennett

et al. 1991

Equine Veterinary Journal

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SummaryHorse articular cartilage glycosaminoglycans (GAGs) were measured in synovial fluids from 48 joints affected with osteoarthritis (OA), 22 normal joints, four joints with osteochondritis, three joints with traumatic arthritis and seven joints infected with bacteria. Serum and urine from individual horses were also examined for the presence of GAGs. High levels of GAGs were found in synovial fluids (SF) from horses with OA. In each case, the level was higher in the synovial fluid than in the serum or urine from the same horse. Horses with OA showed high GAG levels in SF, serum and urine compared to horses with normal and infected joints. High levels were also found in horses with osteochondritis and traumatic arthritis. Levels of synovial fluid GAG reflect cartilage destruction in arthritis and may be useful for monitoring disease progression in the equine species.

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Section: Gag Assaymentioning

confidence: 99%

Glycosaminoglycans in horses with osteoarthritis

Alwan

Carter

Bennett

et al. 1991

Equine Veterinary Journal

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“…GAG content of the constructs was measured using the dimethylmethylene blue dye method. 21 Chondroitin sulfate B (Sigma-Aldrich) was used as a standard in the interpretation of the data. The hydroxyproline content was quantified using the simplified hydroxyproline method as previously reported.…”

Section: Specimen Evaluationmentioning

confidence: 99%

Injectable and Photopolymerizable Tissue-Engineered Auricular Cartilage Using Poly(Ethylene Glycol) Dimethacrylate Copolymer Hydrogels

Papadopoulos

Bichara²,

Zhao³

et al. 2011

Tissue Engineering Part A

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In this study we investigated the histological, biochemical, and integrative features of the neocartilage using swine auricular chondrocytes photoencapsulated into two poly(ethylene glycol) dimethacrylate (PEGDM) copolymer hydrogels of a different degradation profile: degradable (PEG-4,5LA-DM) and nondegradable (PEGDM) macromers in molar ratios of 60:40 and 70:30. Integration of the engineered tissue with existing native cartilage was examined using an articular cartilaginous ring model. Experimental group samples (total n ¼ 96) were implanted subcutaneously into nude mice and harvested at 6, 12, and 18 weeks. Nonimplanted constructs (total n ¼ 16) were used as controls for quantification of DNA, glycosaminoglycan, and hydroxyproline. Histologically, neocartilage resembled both the cellular population and composition of the extracellular matrix of the native swine auricular cartilage. DNA content demonstrated that the photoencapsulated chondrocytes were capable of survival and proliferation over time. Both glycosaminoglycan and hydroxyproline contents appeared higher in the neotissue, which was supported by less degradable PEGDM hydrogel. Integration of neocartilage with surrounding native cartilage improved with time, resulting in the development of tight integration interface. PEGDM copolymer hydrogels can support in vivo chondrogenesis by photoencapsulating auricular chondrocytes.

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“…Type C virus particles (designated FBJ) were identified in the original tumour, suggesting an aetiological relationship (Biskis and Finkel, 1969) which was confirmed by cell-free passage of similar lesions to strains other than the CF1 mouse (Kelloff et al, 1969;Yumoto et al, 1970). Subsequently, neoplastic transformation of rat embryo cells in vitro by FBJ virus was demonstrated by Rhim et al (1969), which, on injection into newborn NIH Swiss mice produced sarcomata of different histological types, including " osteoblastic sarcoma, osteogenic sarcoma and fibrosarcoma with osteoblasts and chondrocytes ".…”

mentioning

confidence: 94%

“…However, to date the virus has revealed no significant leukaemogenic activity, passage through a number of mouse strains having produced only sarcomata (Yumoto et al, 1970).…”

mentioning

confidence: 99%