A new BF F variant by polyacrylamide gel isoelectric focusing

Nakamura, Shinko; Ohue, Osamu; Abe, Kazue

doi:10.1007/bf00284492

Cited by 15 publications

(6 citation statements)

References 17 publications

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“…1 and 2). The BF F"subtype variants" (FBl*; [30,31] and FB2*; 1321) can be distinguished from the common F subtypes by the absence of both the anodic minor band and a split in the major F band (Fig. 2).…”

Section: Demonstration Of Unconverted Factor B After Treatment With Nmentioning

confidence: 99%

Improvement of BF typing and of BF F subtyping after neuraminidase treatment in the unconverted and converted factor B

et al. 1992

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When neuraminidase-treated sera are analyzed by agarose gel isoelectric focusing, the factor B (BF) banding pattern is reduced to predominantly one major band without cathodically positioned bands. This not only makes unequivocal typing of BF allotypes possible but also the reliable distinction of all BF F subtype phenotypes with delimitation of "BF F subtype variants". With this new method, serum aging affects the BF determination to a lesser extent than when applying methods that separate native sera. We show that sialylation is not responsible for the BF F subtype polymorphism. All of the investigated BF allotype bands, including those characteristic of the subtypes, show functional hemolytic activity. The banding pattern after removal of neuraminic acid residues ranges from pH 6.8 to 7.3 for factor B, from pH 5.3 to 5.9 for the Ba fragment, and from pH 8.2 to 8.7 for the Bb fragment. The protein structure of factor B is also discussed. Eliminating the superimposition of bands in different BF allotypes, as demonstrated by these methods, proved to be necessary for the detection of hypomorphic BF gene products (BF QL), which are expressed by assumed BF*Q0 alleles in heterozygous genotypes. This allows investigation of BF*Q0 alleles on a protein level, which complements molecular genetic approaches.

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Section: Demonstration Of Unconverted Factor B After Treatment With Nmentioning

confidence: 99%

Improvement of BF typing and of BF F subtyping after neuraminidase treatment in the unconverted and converted factor B

et al. 1992

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“…[12] for FXIJIB, with the following slight modifications: Gels for FXIIIA contained 5% Ampholine carrier ampholytes (LKB), pH range 4-6, 6-8,3.5-9.5 in a 1:2:1 ratio, 10% glycerol, and 2 M urea. Gels for FXIIIB contained 5 Yo ampholine consisting of 2 parts of pH 4-6, one part of pH 5-7, and one part of 3.5-9.5, 10% glycerol, and 3 M urea.…”

Section: Polyacrylamide Iefmentioning

confidence: 99%

Genetic polymorphisms of the A and B subunits of human coagulation factor XIII in mainland Italy and Sardinia: Description of a new FXIIIA variant allele

Scacchi¹,

Corbo²,

Mureddu³

et al. 1991

Electrophoresis

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The distribution of the two alleles of FXIIIA and the three alleles of FXIIIB were studied in populations from mainland Italy and from Sardinia. The frequencies of the FXIIIA*2 allele were 0.266 and 0.265. The frequencies of FXIIIB*1 were 0.787 and 0.765; of B*2, 0.070 and 0.094; of B*3, 0.143 and 0.141. A new cathodal FXIIIA allele (A*7) was described in the Rome sample. No significant difference in the distribution of allele frequencies for either system was found between the two populations studied. For typing both markers, good results were also obtained by using whole blood instead of plasma.

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“…BF Fbl [13,14] was distinguishable from BF FBI [15] and from the common FB subtype in the Ba fragment. The new designation for these variants will be [see also 16]: 'Fbl' FBI, 'FB 1' -» FB2.…”

Section: Methods Of Bf Typingmentioning

confidence: 99%

Factor B Reference Typing Report

Geserick

Abbal²,

Brenden³

et al. 1990

Complement Inflamm

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In a factor B (BF) Reference Typing of the Vlth Complement Genetics Workshop and Conference, Mainz, FRG, 1989, 99 samples from 13 laboratories, including 18 families, were investigated with the majority of presently known typing procedures. Among the major (‘standard’) allotypes BF S04 was found to be new. For the group of common BF F subtypes samples from 11 laboratories including complete family data from 5 laboratories were compared. The subtypes BF FA and FB were recognized and confirmed to be identical in the samples from all groups. Within a third group rare subtype variants of F and S were compared and characterized. In samples submitted from individuals with assumed non- expressed (BF*Q0) alleles unexpected and hypomorphic gene products were seen. The investigation of DNA samples for restriction fragment length polymorphisms from the same set of individuals revealed a correlation of the Msp 10.7-kb fragment with BF F, and confirmed the correlation of a Taq I 6.6-kb fragment with BF FA.

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A new BF F variant by polyacrylamide gel isoelectric focusing

Cited by 15 publications

References 17 publications

Improvement of BF typing and of BF F subtyping after neuraminidase treatment in the unconverted and converted factor B

Improvement of BF typing and of BF F subtyping after neuraminidase treatment in the unconverted and converted factor B

Genetic polymorphisms of the A and B subunits of human coagulation factor XIII in mainland Italy and Sardinia: Description of a new FXIIIA variant allele

Factor B Reference Typing Report

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