Factor B Reference Typing Report

Geserick, G.; Abbal, M.; Brenden, Martina; Braun-Stilwell, Margot; Mauff, G.; Schröder, Helga; Siemens, I.

doi:10.1159/000463146

Cited by 22 publications

(10 citation statements)

References 10 publications

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“…and stained [14]. For nomenclature rules see Geserick et al [27], BF Subtyping. The BF F samples were character ized by isoelectric focusing in agarose and immuno fixation as previously described by Abbai et al [21].…”

Section: Methodsmentioning

confidence: 99%

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Sarcoidosis and Major Histocompatibility Complex Genes with Special Emphasis on BF F Subtypes

et al. 1991

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Frequencies for HLA class I and II histoglobulins and C4A, C4B, BF complement proteins were performed for 59 sarcoidosis patients. The DR5 allele was present in 55.9% of patients as compared to 31.5% of controls. We noticed that its increase was more relevant in males and in those with a poor prognosis. BF F allele was significnatly overrepresented in patients (29.09 % vs. 19.15 % of control), especially in women. Special emphasis was given to BF F subtyping, to define an association between a particular BF F subtype and patient’s sex or disease outcome.

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Section: Methodsmentioning

confidence: 99%

“…The gel was then dried and colored with Coomassie blue. For nomenclature rules see Geserick et al [27], Statistical Analysis The association degree was estimated by x2 test and Fisher's probability value (double-tailed). Appropriate correction for the de grees of freedom was applied when necessary.…”

Section: Methodsmentioning

confidence: 99%

Sarcoidosis and Major Histocompatibility Complex Genes with Special Emphasis on BF F Subtypes

et al. 1991

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“…These samples included the common phenotypes F, FS, and S, as well as the variants F155 (in the phenotype F155S), F1 (in F1F), F065 (in F065S), and S07 (in SS07). The typing technology and nomenclature were used as published previously [1][2][3].…”

Section: Protein Allotypingmentioning

confidence: 99%

“…Using polyacrylamide gel isoelectric focusing (PAGIF), the BF*F subtypes FA and FB can be identified [2][3][4]. These subtypes as well as the major alleles F and S can also be distinguished directly at the genomic DNA level using PCR followed by restriction fragment analysis of codon 7 with the enzymes MspI (for F/S typing) and BslI (for FA/FB typing), as summarized in table 1 [5,6].…”

Section: Isoelectric Focusing and Pcr Typingmentioning

confidence: 99%

Reference Typing Report for Complement Factor B

Geserick

Schröder²,

Stradmann‐Bellinghausen³

et al. 1998

Exp Clin Immunogenet

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Factor B (BF) reference typing was carried out on the occasion of the VIIth Complement Genetics Workshop in Mainz, May 1998. Two different sets of samples were analysed by agarose electrophoresis and/or isoelectric focusing at the protein level, and by PCR-RFLP analysis at the DNA level. These results confirmed the reliability of the standard agarose electrophoresis technique for the identification of the major BF alleles as well as for the identification of cathodic and anodic variants. However, the exact alphanumeric designation of individual variants relative to the reference distance between alleles S and F1 turned out to be more difficult. Using PCR-RFLP analysis, the common alleles F and S as well as the FA and FB subtypes in 6 samples containing an F allele were all assigned correctly. However, the variants F1 and S07 were not detected by this method, as they could not be distinguished from the accompanying S allele. Therefore, a combined application of all three typing methods is recommended for a reliable identification of factor B alleles, variants and FA/FB subtypes.

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“…In addition, among the six variants submitted to the Vlth Comple ment Genetics Workshop Reference Typing the new allotypes BF*FQL02 (QL = quanti tatively low) and S04 were detected [see also 20], A further variant could not be distin guished by determination of relative electro phoretic migration with sufficient reliability but probably must be classified as 'S02'. A list of presently reported major BF allotypes is given in table 1.…”

Section: Introductionmentioning

confidence: 99%

Factor B (BF) Nomenclature Statement

Geserick¹,

Abbal²,

Mauff³

et al. 1990

Complement Inflamm

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A common nomenclature for factor B (BF) allotypes is recommended as a result of the BF Reference Typing of the Vlth Complement Genetics Workshop and Conference, Mainz, FRG, 1989. It has generally been agreed that the alphanumeric BF nomenclature according to Mauff et al. should also be used in the future for all major BF allotypes distinguishable by standard agarose gel electrophoresis (AGE). The common BF F subtypes and further described rarer subtype variants are not detectable by standard AGE. Therefore, the nomenclature had to be extended. For the subtypes of BF F an alphabetical designation with capital letters will now be used: FA and FB. The designation of the five rarer subtype variants was modified after the reference typing to FBI, FB2, SB1, SB2, and SB3. Hyposynthetic variants detected in samples with previously assumed non-expressed (BF*Q0) alleles are now designated as SQL, M1QL, and M2QL, ‘QL’ characterizing their lower concentration.

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Factor B Reference Typing Report

Cited by 22 publications

References 10 publications

Sarcoidosis and Major Histocompatibility Complex Genes with Special Emphasis on BF F Subtypes

Sarcoidosis and Major Histocompatibility Complex Genes with Special Emphasis on BF F Subtypes

Reference Typing Report for Complement Factor B

Factor B (BF) Nomenclature Statement

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