A new BIS-Andrographolide Ether from <i>Andrographis paniculata</i> Nees and evaluation of anti-HIV activity

Reddy, V. L. Niranjan; Reddy, S. Malla; Ravikanth, V.; Krishnaiah, P.; Goud, T. Venkateshwar; Rao, T. Prabhakar; Ram, Tejomoorty Siva; Gonnade, Rajesh G.; Bhadbhade, Mohan M.; Venkateswarlu, Y.

doi:10.1080/14786410410001709197

Cited by 100 publications

(46 citation statements)

References 13 publications

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“…n the process of osteogensis, Wnt5a antagonizes Wnt/ -catenin signaling through the Wnt/Calcium pathway [ ] and -catenin degradation by promoting b [ 4]. tudies ha e shown that haploinsuf iciency of -b can partially rescue dwar ism caused by c P-dependent protein inase de iciency [ 5]. n the -catenin -independent pathway, Wnt5a acti ated Rac by the ac uirement of Frizzled2 (Fzd2) through a clathrinmediated route in response [ ].…”

Section: Discussionmentioning

confidence: 99%

Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro

Jiang¹,

Zhou²,

Huang³

et al. 2015

Cell Physiol Biochem

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Background/Aims: Osteoporosis is a metabolic bone disorders that tortures about millions of people worldwide. Recent studies showed that Andrographolide (AP) is a promising natural compound for the treatment of osteoclast-related bone diseases. However, its potential in treatment of osteoporosis has not been fully explored. Methods: In this study, the effect of AP on osteoblasts metabolism was investigated via the detection of cell proliferation, cell viability, ALP activity, the expression of osteogenic specific genes including runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP), osteocalcin (OCN), Bone morphogenic protein-2 (BMP2) and Alkaline phosphatase(ALP) for 3, 5 and 7 days respectively. Further exploration of the association of AP with WNT/β-catenin signaling pathway was performed by examination of the expression of WNT related genes and proteins. Results: Results showed that AP of 4.46 and 8.92 µM, especially 8.92 µM was beneficial to osteogenic differentiation by upregulating ALP activity and expression of osteogenic related genes (P<0.05). Pathway analyses identify canonical WNT/β-catenin pathway as an important mediator in AP-induced osteogenesis. Conclusion: This study indicates that AP exerts its pro-osteogenic potential via activation of the WNT/β-catenin in osteoblasts and thus may represent a candidate of therapeutic agent for osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro

Jiang¹,

Zhou²,

Huang³

et al. 2015

Cell Physiol Biochem

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“…Andrographolide, and 14-deoxy-11,12-didehydroandrographolide have been reported to exhibit anti-HIV activity [34]. Similarly, methanolic extract of M. charantia was found to contain terpenes, saponins and steroids [35,36].…”

Section: Resultsmentioning

confidence: 99%

Screening of anti-dengue activity in methanolic extracts of medicinal plants

Tang

Ling

Koh

et al. 2012

BMC Complement Altern Med

153

113

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BackgroundDengue fever regardless of its serotypes has been the most prevalent arthropod-borne viral diseases among the world population. The development of a dengue vaccine is complicated by the antibody-dependent enhancement effect. Thus, the development of a plant-based antiviral preparation promises a more potential alternative in combating dengue disease.MethodsPresent studies investigated the antiviral effects of standardised methanolic extracts of Andrographis paniculata, Citrus limon, Cymbopogon citratus, Momordica charantia, Ocimum sanctum and Pelargonium citrosum on dengue virus serotype 1 (DENV-1).ResultsO. sanctum contained 88.6% of total flavonoids content, an amount that was the highest among all the six plants tested while the least was detected in M. charantia. In this study, the maximum non-toxic dose (MNTD) of the six medicinal plants was determined by testing the methanolic extracts against Vero E6 cells in vitro. Studies also determined that the MNTD of methanolic extract was in the decreasing order of M. charantia >C. limon >P. citrosum, O. sanctum >A. paniculata >C. citratus. Antiviral assay based on cytopathic effects (CPE) denoted by degree of inhibition upon treating DENV1-infected Vero E6 cells with MNTD of six medicinal plants showed that A. paniculata has the most antiviral inhibitory effects followed by M. charantia. These results were further verified with an in vitro inhibition assay using MTT, in which 113.0% and 98.0% of cell viability were recorded as opposed to 44.6% in DENV-1 infected cells. Although methanolic extracts of O. sanctum and C. citratus showed slight inhibition effect based on CPE, a significant inhibition was not reflected in MTT assay. Methanolic extracts of C. limon and P. citrosum did not prevent cytopathic effects or cell death from DENV-1.ConclusionsThe methanol extracts of A. paniculata and M. charantia possess the ability of inhibiting the activity of DENV-1 in in vitro assays. Both of these plants are worth to be further investigated and might be advantageous as an alternative for dengue treatment.

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“…Some of these phytochemicals exhibited a wide range of biological activities such as antiviral , antibacterial , anticancer , antioxidant , and anti‐inflammatory as well as inhibitory activities of the phosphorylation of mitogen‐activated protein kinases . The rich terpenoids and flavones are found to be responsible for the therapeutic effects of leaves of AP . In order to further study the biochemical properties of terpenoids and flavones, and broaden their pharmacological applications, it is necessary to obtain large quantities of highly purified compounds.…”

Section: Introductionmentioning

confidence: 99%

Separation of five compounds from leaves of Andrographis paniculata (Burm. f.) Nees by off‐line two‐dimensional high‐speed counter‐current chromatography combined with gradient and recycling elution

Zhang

Liu

et al. 2015

J of Separation Science

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An off-line two-dimensional high-speed counter-current chromatography method combined with gradient and recycling elution mode was established to isolate terpenoids and flavones from the leaves of Andrographis paniculata (Burm. f.) Nees. By using the solvent systems composed of n-hexane/ethyl acetate/methanol/water with different volume ratios, five compounds including roseooside, 5,4'-dihydroxyflavonoid-7-O-β-d-pyranglucuronatebutylester, 7,8-dimethoxy-2'-hydroxy-5-O-β-d-glucopyranosyloxyflavon, 14-deoxyandrographiside, and andrographolide were successfully isolated. Purities of these isolated compounds were all over 95% as determined by high-performance liquid chromatography. Their structures were identified by UV, mass spectrometry, and (1) H NMR spectroscopy. It has been demonstrated that the combination of off-line two-dimensional high-speed counter-current chromatography with different elution modes is an efficient technique to isolate compounds from complex natural product extracts.

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A new BIS-Andrographolide Ether from Andrographis paniculata Nees and evaluation of anti-HIV activity

Cited by 100 publications

References 13 publications

Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro

Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro

Screening of anti-dengue activity in methanolic extracts of medicinal plants

Separation of five compounds from leaves of Andrographis paniculata (Burm. f.) Nees by off‐line two‐dimensional high‐speed counter‐current chromatography combined with gradient and recycling elution

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