A new chemically defined medium for the growth and sporulation of Bacillus cereus strains in anaerobiosis

Abbas, Amina Aicha; Planchon, Stella; Jobin, Michel-Philippe; Schmitt, Philippe

doi:10.1016/j.mimet.2014.07.006

Cited by 9 publications

(5 citation statements)

References 35 publications

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“…Based on MOD a chemically defined medium was used for B. cereus to demonstrate anaerobic cultivation conditions in Hungate tubes. The medium contained low concentrations of glucose (10 mM) and amino acids (2.5 gL −1 ) [72]. Monteiro et al developed and optimized a chemically defined medium for B. subtilis by varying the concentrations of vitamins, carbon, nitrogen and Ca 2+ .…”

Section: Defined Mediamentioning

confidence: 99%

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Endospore production of Bacillus spp. for industrial use

Biermann,

Beutel

2023

Engineering in Life Sciences

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The increased occurrence of antibiotic resistance and the harmful use of pesticides are a major problem of modern times. A ban on the use of antibiotics as growth promoters in animal breeding has put a focus on the probiotics market. Probiotic food supplements are versatile and show promising results in animal and human nutrition. Chemical pesticides can be substituted by biopesticides, which are very effective against various pests in plants due to increased research. What these fields have in common is the use of spore‐forming bacteria. The endospore‐forming Bacillus spp. belonging to this group offer an effective solution to the aforementioned problems. Therefore, the biotechnological production of sufficient qualities of such endospores has become an innovative and financially viable field of research. In this review, the production of different Bacillus spp. endospores will be reviewed. For this purpose, the media compositions, cultivation conditions and bioprocess optimization methods of the last 20 years are presented and reflected.

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Section: Defined Mediamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endospore production of Bacillus spp. for industrial use

Biermann,

Beutel

2023

Engineering in Life Sciences

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“…The growth and sporulation model (equations 1, 2, and 5) was used to fit the kinetics of B. subtilis BSB1 cultivated in whey and of Bacillus licheniformis Ad978 cultivated in batch in Hageman medium supplemented with 1% brain heart infusion (BHI) at two temperatures. Kinetics from the literature (24) were used also to fit the model, like the kinetics obtained with Bacillus cereus AH187 cultivated in the chemically defined MODS medium (50) in aerobiosis at 37°C (Fig. 2).…”

Section: Importancementioning

confidence: 99%

Differentiation of Vegetative Cells into Spores: a Kinetic Model Applied to Bacillus subtilis

Gauvry

Mathot

Couvert

et al. 2019

Appl Environ Microbiol

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Spore-forming bacteria are natural contaminants of food raw materials, and sporulation can occur in many environments from farm to fork. In order to characterize and to predict spore formation over time, we developed a model that describes both the kinetics of growth and the differentiation of vegetative cells into spores. The model is based on a classical growth model and enables description of the kinetics of sporulation with the addition of three parameters specific to sporulation. Two parameters are related to the probability of each vegetative cell to commit to sporulation and to form a spore, and the last one is related to the time needed to form a spore once the cell is committed to sporulation. The goodness of fit of this growth-sporulation model was assessed using growth-sporulation kinetics at various temperatures in laboratory medium or in whey for Bacillus subtilis, Bacillus cereus, and Bacillus licheniformis. The model accurately describes the kinetics in these different conditions, with a mean error lower than 0.78 log 10 CFU/ml for the growth and 1.08 log 10 CFU/ml for the sporulation. The biological meaning of the parameters was validated with a derivative strain of Bacillus subtilis 168 which produces green fluorescent protein at the initiation of sporulation. This model provides physiological information on the spore formation and on the temporal abilities of vegetative cells to differentiate into spores and reveals the heterogeneity of spore formation during and after growth. Citation Gauvry E, Mathot A-G, Couvert O, Leguérinel I, Jules M, Coroller L. 2019. Differentiation of vegetative cells into spores: a kinetic model applied to Bacillus subtilis. Appl Environ Microbiol 85:e00322-19. https://doi .on July 10, 2020 by guest http://aem.asm.org/ Downloaded from RESULTSModel development and experimental strategy. The growth of vegetative cells was described by a modified logistic model (equation 1) and their differentiation into spores over time with specific sporulation parameters: the probability (t max , , and P max ; see below) of vegetative cells to give a mature spore (heat resistant) during the incubation and the time needed for the spore formation (t f

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“…For assay of the spores biofilm, the sporulation Casein-Casein-Yeast (CCY) medium (Sigma-Aldrich, USA) was used. The method of spore production was introduced by Abbas et al [18] and modified as follows. To obtain spores form vegetative cells, both bacterial strains were incubated in a CCY medium for 24 hours.…”

Section: Determining the Biofilm Biomass Formation Capacitymentioning

confidence: 99%

Biofilm formation capacity of Bacillus cereus on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials

Oder

Fink

2019

Sanitarno Inženirstvo International Journal of Sanitary Engineering Research

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Biofilms on food contact materials represent public health issues because they are resistant to cleaning and disinfection. This study aims to assess the Bacillus cereus biofilm formation capacity on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials. The biofilm biomass was analysed with the crystal violet assay method. We used the standard strain B. cereus CCM 2010, wild strain B. cereus 100 and spores of those two strains. The results show that both the vegetative form the bacteria and it spores form large amounts of biofilm on silicone, followed by polyethylene terephthalate, Teflon, and aluminium. More detailed analysis has shown that spores form more biomass on all materials in comparison to the vegetative form and that the standard strains form low levels of biofilm in contrast to the wild strains. Selecting proper material with the lowest biofilm formation potential can prevent or reduce food contamination and consequently increase food safety.

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A new chemically defined medium for the growth and sporulation of Bacillus cereus strains in anaerobiosis

Abstract: A new chemically defined liquid medium, MODS, was developed for the aerobic growth and anaerobic growth and sporulation of Bacillus cereus strains. The comparison of sporulation capacity of 18 strains of B. cereus has shown effective growth and spore production in anaerobiosis..

Cited by 9 publications

References 35 publications

Endospore production of Bacillus spp. for industrial use

Endospore production of Bacillus spp. for industrial use

Differentiation of Vegetative Cells into Spores: a Kinetic Model Applied to Bacillus subtilis

Biofilm formation capacity of Bacillus cereus on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials

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