A new chloramphenicol and florfenicol resistance gene flanked by two integron structures in<i>Salmonella typhimurium</i>DT104

Arcangioli, Marie-Anne; Leroy-Sétrin, Sabine; Martel, Jean-Louis; Chaslus-Dancla, Élisabeth

doi:10.1111/j.1574-6968.1999.tb13586.x

Cited by 147 publications

(41 citation statements)

References 22 publications

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“…Several possible explanations can be put forward, most of which would involve the presence of a particular protein coding gene within the MDR region. For example, the floR gene, which conveys cross-resistance to chloramphenicol and florfenicol encodes a membrane spanning efflux pump protein, which may possess other transport functions [37]. There are also a small number of gene products, namely ORF1, 2, 5, 6, as well as a groEL gene fragment, encoded in the MDR region, which currently have unknown functions.…”

Section: Discussionmentioning

confidence: 99%

Identification of Virulence Properties in Salmonella Typhimurium DT104 Using Caenorhabditis elegans

et al. 2013

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Salmonella enterica serover Typhimurium definitive phage type DT104, resistant to multiple antibiotics, is one of the most widespread Salmonella species in human infection worldwide. Although several cohort studies indicate that DT104 carrying the multidrug resistance (MDR) locus on salmonella genomic island 1 is a possible hyper-virulent strain compared to DT104 strains without MDR, or other Salmonella enterica serotypes, existing experimental evidence regarding virulence properties associated with the MDR region is controversial. To address this question, we constructed an isogenic MDR deletion (∆MDR) mutant strain of DT104, SNS12, by allelic exchange and used Caenorhabditis elegans as a host model to assess differences in virulence between these two strains. SNS12 exhibited decreased virulence in C. elegans, and we observed increased colonization and proliferation of the intestine of C. elegans by DT104. The immune response against MDR-carrying DT104 appears to function through a non-canonical Unfolded Protein Response (UPR) pathway, namely prion-like-(QN-rich)-domain-bearing protein pathway (PQN), in a ced-1 dependent manner in C. elegans. Further, we also demonstrate that genes of the PQN pathway and antimicrobial peptide gene abf-2, are expressed at higher transcriptional levels in worms immediately following exposure to DT104, in comparison with worms exposed to SNS12. Altogether, our results suggest that the MDR region of Salmonella Typhimurium DT104 has a direct role in virulence against Caenorhabditis elegans.

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Section: Discussionmentioning

confidence: 99%

Identification of Virulence Properties in Salmonella Typhimurium DT104 Using Caenorhabditis elegans

et al. 2013

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“…This gene was first identified in Salmonella typhimurium DT104 in 1999 and has ever since been detected in Escherichia coli and P. multocida isolated from livestock and aquaculture settings 47–50 . It is possible that the emergence and spreading of floR is due to the selection by florfenicol, since this antibiotic has been extensively used in veterinary medicine, especially in aquaculture settings 49,51 .…”

Section: Discussionmentioning

confidence: 99%

Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn43716385

et al. 2018

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Pseudomonas aeruginosa can cause life-threatening infections in immunocompromised patients. The first-line agents to treat P. aeruginosa infections are carbapenems. However, the emergence of carbapenem-resistant P. aeruginosa strains greatly compromised the effectiveness of carbapenem treatment, which makes the surveillance on their spreading and transmission important. Here we characterized the full-length genomes of two carbapenem-resistant P. aeruginosa clinical isolates that are capable of producing New Delhi metallo-β-lactamase-1 (NDM-1). We show that blaNDM-1 is carried by a novel integrative and conjugative element (ICE) ICETn43716385, which also carries the macrolide resistance gene msr(E) and the florfenicol resistance gene floR. By exogenously expressing msr(E) in P. aeruginosa laboratory strains, we show that Msr(E) can abolish azithromycin-mediated quorum sensing inhibition in vitro and anti-Pseudomonas effect in vivo. We conclude that ICEs are important in transmitting carbapenem resistance, and that anti-virulence treatment of P. aeruginosa infections using sub-inhibitory concentrations of macrolides can be challenged by horizontal gene transfer.

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“…The pandemic spread of S . Typhimurium DT104, for example, has caused a high number of foodborne disease outbreaks over the last two decades and is resistant to four of the five most commonly used antibiotics in veterinary medicine (tetracycline, β-lactams, aminoglycosides and sulfonamides) 106 , 107 . These multidrug-resistant strains are more virulent and cause increased hospitalizations and bacteremia 108 , 109 .…”

Section: Salmonella (2012): the Trojan Horsementioning

confidence: 99%

Rise of the microbes

Mahan

Kubicek-Sutherland²,

Heithoff³

2013

Virulence

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Infectious diseases continue to plague the modern world. In the evolutionary arms race of pathogen emergence, the rules of engagement appear to have suddenly changed. Human activities have collided with nature to hasten the emergence of more potent pathogens from natural microbial populations. This is evident in recent infectious disease outbreaks, the events that led to their origin, and lessons learned: influenza (2009), meningitis (Africa, 2009), cholera (Haiti, 2010), E. coli (Germany, 2011) and Salmonella (USA, 2012). Developing a comprehensive control plan requires an understanding of the genetics, epidemiology and evolution of emergent pathogens for which humans have little or no pre-existing immunity. As we plot our next move, nature’s genetic lottery continues, providing the fuel to transform the most unlikely infectious disease scenarios into reality.

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A new chloramphenicol and florfenicol resistance gene flanked by two integron structures inSalmonella typhimuriumDT104

Cited by 147 publications

References 22 publications

Identification of Virulence Properties in Salmonella Typhimurium DT104 Using Caenorhabditis elegans

Identification of Virulence Properties in Salmonella Typhimurium DT104 Using Caenorhabditis elegans

Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn43716385

Rise of the microbes

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