A new class of phospholipase A2 substrates: kinetics of the phospholipase A2 catalyzed hydrolysis of 3-(acyloxy)-4-nitrobenzoic acids

Proteomic and biological characterization of the venom of the redtail coral snake, Micrurus mipartitus (Elapidae), from Colombia and Costa Rica

“…Phospholipase A 2 (PLA 2 ) activity of the venoms was assayed on the synthetic substrate 4-nitro-3-octanoyloxy-benzoic acid [24,25]. Variable amounts of venoms (in 25 μL of 10 mM Tris, 10 mM CaCl 2 , 100 mM NaCl, pH 8.0 buffer) were mixed with 25 μL of substrate (1 mg/ml) and 250 μL of the same buffer, and incubated for 60 min at 37°C, in triplicate wells of a microplate.…”

Phospholipases A2 from Callosellasma rhodostoma venom gland

“…4-Nitro-3-(octanoyloxy)benzoic acid (NOB) is a gift from F. J. Ketzdy (The Upjohn Company, Kalamazoo, MI, USA) [7] and is now available from Sigma. Other reagents and buffers are of reagent grade.…”

Native state dynamics affects the folding transition of porcine pancreatic phospholipase A2

“…For NMR and some CD measurements (see below), purified ppPLA 2 was extensively dialyzed against water and lyophilized. The catalytic activity was tested by 4-nitro-3-octanoyloxybenzoate (NOB, Sigma) [28] as substrate. Assays were performed as described [29], but in a continuous setup and at varied substrate concentrations.…”