A new colorimetric nucleic acid hybridization assay for Listeria in foods

King, W.A.; Raposa, S. M.; Warshaw, J. E.; Johnson, Andrew; Halbert, Donald N.; Klinger, Jeffrey D.

doi:10.1016/0168-1605(89)90017-2

Cited by 57 publications

(31 citation statements)

References 7 publications

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“…A commercially available DNA probe assay (Gene-Trak Systems, Framingham, Mass.) uses a fluorescein-labeled DNA probe targeted to a Listeria species-specific rRNA sequence for rapid detection of Listeria species in foods (82,84).…”

Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

Epidemiology of human listeriosis

1991

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During the 1980s, investigation of several large epidemics of listeriosis confirmed that transmission of L. monocytogenes in food causes human disease. Progress in laboratory detection and subtyping of the organism has enhanced our ability to compare human and environmental isolates of L. monocytogenes. Transmission by foodborne organisms is now recognized as causing both epidemic and sporadic listeriosis. Continued study of dietary risk factors associated with listeriosis is needed in order to develop dietary recommendations for the expanding population at increased risk of disease. Current research application of new molecular methods to the study of L. monocytogenes may improve the ability to diagnose pregnancy-associated disease and permit the rapid detection and control of L. monocytogenes in the food supply.

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Section: Microbiology Of L Monocytogenesmentioning

confidence: 99%

Epidemiology of human listeriosis

1991

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“…Because the nucleic acids contain all the primary information that specifies the complete make-up and characteristics of an organism, probes should be able to be designed which are not only entirely specific, but related to the organism's genealogy and are specific at the different taxonomic levels of genus and species, or for a particular gene of interest, such as a pathogenic factor. Many nucleic acid probes have now been produced, mainly in research laboratories, although some have been commercialized (Klinger et a/., 1988;Barry, Powell and Gannon, 1990). However, probe hybridization is invariably measured using 32p.…”

Section: Primary Enzyme Involvement For Bacterial Detectionmentioning

confidence: 99%

The Influence of Biotechnological Developments on Cheese Manufacture

Law

Mulholland

1991

Biotechnology and Genetic Engineering Reviews

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“…Methods for obtaining rapid results equivalent to total viable counts based on assays of microbial ATP (La Rocco et al 1986;Webster et al 1988) or epifluorescent microscopy (Pettipher and Rodrigues 1982;Rodrigues and Kroll 1988) have been successfully developed. Genus-or species-specific methods for food-borne bacterial pathogens have also been developed, based on immunological (Mattingly 1984;Beumer and Brinkman 1989) or nucleic acid hybridisation probe (Fitts et al 1983;Klinger et al 1988) specificity.…”

Section: Introductionmentioning

confidence: 99%

A semi-homogeneous amperometric immunosensor for protein A-bearing Staphylococcus aureus in foods

Mirhabibollahi

Brooks

Kroll

1990

Appl Microbiol Biotechnol

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A semi-homogeneous amperometric immunosensor specific to the protein A of Staphylococcus aureus was developed using direct electrochemical detection of phenol produced by alkaline phosphatase from phenyl phosphate. The immunosensor could reliably detect strains of protein A-bearing S. aureus in pure cultures at ca. 10(4) cfu/ml, and at ca. 10(5) cfu/g or ml in various food samples. Due to its semi-homogeneous nature, the system was very simple, easy to operate, and labour-saving. The good correlation between the amperometric current generated by the immunosensor and plate counts illustrated the potential usefulness of this simple system. It proved to be a reliable 24-h detection method for food samples containing very low numbers of protein A-bearing S. aureus after pre-enrichment, as it was able to detect cells that could not directly be enumerated by plate counts.

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A new colorimetric nucleic acid hybridization assay for Listeria in foods

Cited by 57 publications

References 7 publications

Epidemiology of human listeriosis

Epidemiology of human listeriosis

The Influence of Biotechnological Developments on Cheese Manufacture

A semi-homogeneous amperometric immunosensor for protein A-bearing Staphylococcus aureus in foods

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