A new corneal epithelial biomimetic 3D model for in vitro eye toxicity assessment: Development, characterization and applicability

Silva, Artur Christian Garcia da; Chialchia, Adrienny Rodrigues; Castro, Elisandra Gava de; Silva, Marlos R.L. e; Arantes, Diego Antônio Costa; Batista, Aline Carvalho; Kitten, Gregory T.; Valadares, Marize Campos

doi:10.1016/j.tiv.2019.104666

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…Due to bureaucratic issues, ready-to-use commercially available tissue models are not obtainable in Brazil and South America. This situation led to efforts to develop and commercialize a novel 3D tissue model that could be available in the region (da Silva et al 2020). This in vitro 3D model is reconstructed from the HaCaT cell line, which is a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin.…”

Section: In Vitro 3d Corneal Epithelial Model-based Eye Irritation Testsmentioning

confidence: 99%

In vitro reconstructed 3D corneal tissue models for ocular toxicology and ophthalmic drug development

Kaluzhny

Klausner

2021

In Vitro Cell.Dev.Biol.-Animal

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Testing of all manufactured products and their ingredients for eye irritation is a regulatory requirement. In the last two decades, the development of alternatives to the in vivo Draize eye irritation test method has substantially advanced due to the improvements in primary cell isolation, cell culture techniques, and media, which have led to improved in vitro corneal tissue models and test methods. Most in vitro models for ocular toxicology attempt to reproduce the corneal epithelial tissue which consists of 4-5 layers of non-keratinized corneal epithelial cells that form tight junctions, thereby limiting the penetration of chemicals, xenobiotics, and pharmaceuticals. Also, significant efforts have been directed toward the development of more complex threedimensional (3D) equivalents to study wound healing, drug permeation, and bioavailability. This review focuses on in vitro reconstructed 3D corneal tissue models and their utilization in ocular toxicology as well as their application to pharmacology and ophthalmic research. Current human 3D corneal epithelial cell culture models have replaced in vivo animal eye irritation tests for many applications, and substantial validation efforts are in progress to verify and approve alternative eye irritation tests for widespread use. The validation of drug absorption models and further development of models and test methods for many ophthalmic and ocular disease applications is required.

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Section: In Vitro 3d Corneal Epithelial Model-based Eye Irritation Testsmentioning

confidence: 99%

In vitro reconstructed 3D corneal tissue models for ocular toxicology and ophthalmic drug development

Kaluzhny

Klausner

2021

In Vitro Cell.Dev.Biol.-Animal

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“…In the first culture type, cell-cell interactions between corneal fibroblasts and epithelial cells are analyzed, which can also be used as a so-called wound and healing model of the cornea [ 54 ]. 3D cell culture models with a single cell type can be established for example within an air-liquid interface model, and drug effects, injury, or chemical noxious agents can be studied [ 74 , 75 , 76 , 77 ], (reviewed in [ 54 ]). Pluripotent stem cells can differentiate into any cell type and acquire so-called organ-like structures [ 78 ].…”

Section: Development Of 3d Cell Culture Modelsmentioning

confidence: 99%

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

Aydin

Dietrich

Witt

et al. 2021

IJMS

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There is a lack of knowledge regarding the connection between the ocular and nasal epithelia. This narrative review focuses on conjunctival, corneal, ultrastructural corneal stroma, and nasal epithelia as well as an introduction into their interconnections. We describe in detail the morphology and physiology of the ocular surface, the nasolacrimal ducts, and the nasal cavity. This knowledge provides a basis for functional studies and the development of relevant cell culture models that can be used to investigate the pathogenesis of diseases related to these complex structures. Moreover, we also provide a state-of-the-art overview regarding the development of 3D culture models, which allow for addressing research questions in models resembling the in vivo situation. In particular, we give an overview of the current developments of corneal 3D and organoid models, as well as 3D cell culture models of epithelia with goblet cells (conjunctiva and nasal cavity). The benefits and shortcomings of these cell culture models are discussed. As examples for pathogens related to ocular and nasal epithelia, we discuss infections caused by adenovirus and measles virus. In addition to pathogens, also external triggers such as allergens can cause rhinoconjunctivitis. These diseases exemplify the interconnections between the ocular surface and nasal epithelia in a molecular and clinical context. With a final translational section on optical coherence tomography (OCT), we provide an overview about the applicability of this technique in basic research and clinical ophthalmology. The techniques presented herein will be instrumental in further elucidating the functional interrelations and crosstalk between ocular and nasal epithelia.

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“…A reconstructed human corneal epithelial model (EpiOcular™) was developed by MatTek Life Sciences and consisted of human corneal epithelial cells in 3D cultures that formed a stratified squamous epithelium (Kaluzhny et al, 2018). This model was used as a screening tool for identification of chemicals with the potential for ocular surface toxicity (Garcia et al, 2019;da Silva et al, 2020). Also, a 3D corneal epithelial tissue model was used to study oxidative stress-related corneal epithelial injuries (Kaluzhny et al, 2020).…”

Section: D Models With a Single Cell Typementioning

confidence: 99%

3D in vitro corneal models: A review of current technologies

Shiju

Oliveira

Wilson

2020

Experimental Eye Research

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A new corneal epithelial biomimetic 3D model for in vitro eye toxicity assessment: Development, characterization and applicability

Cited by 5 publications

References 24 publications

In vitro reconstructed 3D corneal tissue models for ocular toxicology and ophthalmic drug development

In vitro reconstructed 3D corneal tissue models for ocular toxicology and ophthalmic drug development

The Communication between Ocular Surface and Nasal Epithelia in 3D Cell Culture Technology for Translational Research: A Narrative Review

3D in vitro corneal models: A review of current technologies

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