Pepper (Piper nigrum L.) is an important cash crop for smallholders in the state of Sarawak, Malaysia. At present, there are more than eight pepper cultivars being planted in the state since the 1950s. In this study, the unique spectral signatures of three high yielding pepper cultivars in Malaysia were examined using a ground-based spectral sensor to detect varietal differences. Concurrent foliar spectral signatures, chlorophyll concentration, and Normalized Difference Vegetation Index (NDVI) of cultivar Kuching, Semongok Aman and Semongok Emas were recorded under normal field conditions. Foliar chlorophyll concentration and NDVI results showed that all the matured pepper vines under this assessment were in healthy condition. From a rough observation, except for a slight increase in the visible (VIS) region of the spectrum (500 to 699 nm) for cultivar Semongok Aman and Semongok Emas, spectral reflectance of all three pepper cultivars were similar in the near-infrared region (700 to 1000 nm). The results for First Derivative Curves analysis indicated that the 684.5 to 756.5 nm region showed the highest separation followed by region with the second lowest separation (504.5 to 540.5 nm). The highest value was obtained in the Red Edge Position (REP) for cultivar Kuching, Semongok Aman and Semongok Emas which were 709, 706 and 710, respectively. The highest value obtained in the VIS region for cultivar Kuching, Semongok Aman and Semongok Emas were 523, 524 and 527, respectively. These fundamental findings suggested that it is possible to discriminate pepper cultivars through field spectroscopy and first derivative analysis. In addition, this information may be used for further studies related to precision agriculture particularly in integrated nutrient and disease management.