Multiple Input Multiple Output (MIMO) system has several input and output antennas for executing the data transmission. Channel Estimation (CE) is required in MIMO, to achieve the effective signal transmission over the various amount of antennas. By using CE over the MIMO, the noiseless data transmission is performed. Hence in this paper, a Multi-layer Neural Network (MNN) is used for identifying the CE and this system is named as Multi-layer Neural Network-MIMO-Digital Filter (MNN-MIMO-CE) is proposed for blind channel equalization. The MNN-MIMO-CE has Feed forward Artificial Neural Network (FANN) with back propagation in Levenberg-Marquardt (LM) algorithm and it has two processes MNN training and MNN testing. LM algorithm is used to train the MNN. These processes are used to provide the CE for different combination of antennas. The performance of the MNN-MIMO-CE method is evaluated in comparison with the existing method [25] through simulations using BER as the performance measure.