A new diet for yeast to improve biofuel production

Galazka, Jonathan M.; Cate, J.H.D.

doi:10.4161/bbug.2.4.15624

Cited by 3 publications

(1 citation statement)

References 54 publications

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“…In microorganisms yeast, the cellobiose utilization pathway usually has two different strategies: (1) cellobiose is first hydrolysed extracellularly to glucose, followed by being transported into cells such as Candida wickerhamii; (2) cellobiose is directly transported into cells and hydrolysed by intracellular b-glucosidase for further mechanism such as Candida queiroziae [5,19,20]. In our work, b-glucosidase, which is responsible for cellobiose hydrolysis, was only detected intracellularly in C. curvatus (unpublished data).…”

Section: Sugar Consumption By C Curvatusmentioning

confidence: 99%

Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Cryptococcus curvatus

Zheng

Xiong

et al. 2014

Biomass and Bioenergy

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Section: Sugar Consumption By C Curvatusmentioning

confidence: 99%

Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Cryptococcus curvatus

Zheng

Xiong

et al. 2014

Biomass and Bioenergy

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An evaluation of cellulose saccharification and fermentation with an engineered Saccharomyces cerevisiae capable of cellobiose and xylose utilization

Fox

Levine

Blanch

et al. 2012

Biotechnology Journal

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Commercial-scale cellulosic ethanol production has been hindered by high costs associated with cellulose-to-glucose conversion and hexose and pentose co-fermentation. Simultaneous saccharification and fermentation (SSF) with a yeast strain capable of xylose and cellobiose co-utilization has been proposed as a possible avenue to reduce these costs. The recently developed DA24-16 strain of Saccharomyces cerevisiae incorporates a xylose assimilation pathway and a cellodextrin transporter (CDT) that permit rapid growth on xylose and cellobiose. In the current work, a mechanistic kinetic model of cellulase-catalyzed hydrolysis of cellulose was combined with a multi-substrate model of microbial growth to investigate the ability of DA24-16 and improved cellobiose-consuming strains to obviate the need for exogenously added β-glucosidase and to assess the impact of cellobiose utilization on SSF and separate hydrolysis and fermentation (SHF). Results indicate that improved CDT-containing strains capable of growing on cellobiose as rapidly as on glucose produced ethanol nearly as rapidly as non-CDT-containing yeast supplemented with β-glucosidase. In producing 75 g/L ethanol, SSF with any strain did not result in shorter residence times than SHF with a 12 h saccharification step. Strains with improved cellobiose utilization are therefore unlikely to allow higher titers to be reached more quickly in SSF than in SHF.

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Lignocellulosic biofuel properties and reactivity analyzed by thermogravimetric analysis (TGA) toward zero carbon scheme: A critical review

Aniza,

Chen,

Kwon

et al. 2024

Energy Conversion and Management: X

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A new diet for yeast to improve biofuel production

Cited by 3 publications

References 54 publications

Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Cryptococcus curvatus

Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Cryptococcus curvatus

An evaluation of cellulose saccharification and fermentation with an engineered Saccharomyces cerevisiae capable of cellobiose and xylose utilization

Lignocellulosic biofuel properties and reactivity analyzed by thermogravimetric analysis (TGA) toward zero carbon scheme: A critical review

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