A new division of bacterial UvrA homologues

Abstract

The UvrA protein is a DNA-binding and damage-recognition enzyme which participates in the prokaryotic type nucleotide excision repair (NER) pathway. It has recently been noted that some bacterial genomes comprise additional uvrA genes which encode five distinct types of UvrA homologue. We investigated the sequences of over 2400 bacterial genomes and found 130 examples of bacteria containing uvrA 2 genes. The sequence analyses conducted on these UvrA homologues revealed that the previously established division of UvrA proteins might be based on some incorrect assumptions. In this paper, we present the reasons for our creation of a new division of UvrA homologues and a description of the four UvrA classes we have created.

The UvrA‐like protein Ecm16 requires ATPase activity to render resistance against echinomycin

“…In addition to the canonical UvrA, several bacterial phyla (including Actinobacteria , Firmicutes , Bacteroidetes , and Proteobacteria ) also express a class II UvrA, which are further subdivided into class IIa and IIb (Marszałkowska et al, 2013). The UvrA homologs found in Streptomyces that produce DNA intercalator drugs are class IIa UvrA (Ogawara, 2019; Zhang et al, 2013).…”

“…The UvrA homologs found in Streptomyces that produce DNA intercalator drugs are class IIa UvrA (Ogawara, 2019; Zhang et al, 2013). Compared to the class I UvrA protein, class IIa UvrA lacks the first zinc finger motif and the UvrB‐binding domain (Marszałkowska et al, 2013). Class IIa UvrA proteins share ~40% sequence identity with the class I UvrA.…”

Mycothiol Peroxidase Activity as a Part of the Self-Resistance Mechanisms against the Antitumor Antibiotic Cosmomycin D

“…The protein encoded by the cosU gene has a high similarity to UvrA proteins; however, it contains deletions of the UvrB-binding domain and the first zinc finger motif. For this reason, the protein function is not involved in NER and it is classified as a UvrA-like class IIa protein ( 43 ). Proteins of the latter class have been shown to behave in vitro like ATP-dependent DNA-binding proteins, such as that of S. peucetius from the daunorubicin cluster ( 10 , 15 ), the nogalamycin produced by Streptomyces nogalater ( 44 ), the product of cmrX that confers resistance to chromomycin in Streptomyces griseus subsp.…”

Class II UvrA protein Ecm16 requires ATPase activity to render resistance against echinomycin

“…In addition to the canonical UvrA, several bacterial phyla (including Actinobacteria, Firmicutes, Bacteroidetes and Proteobacteria) also express a class II UvrA (47). The UvrA homologs found in Streptomyces that produce DNA intercalator drugs are class II UvrA (referred here as UvrA2) (1, 48).…”