A new electrochemical biosensor for DNA detection based on molecular recognition and lead sulfide nanoparticles

Fan, Hao; Zhao, Kun; Lin, Yan; Wang, Xiaoyun; Wu, Bo; Li, Qianggen; Cheng, Lin

doi:10.1016/j.ab.2011.08.005

Cited by 13 publications

(8 citation statements)

References 26 publications

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“…Several groups have developed voltammetric biosensors to detect DNA using different surface modification to improve sensitivity and analytical performances as presented in Table S1 on the Supplementary Information [34][35][36][37][38] . As an example, Bo et al 35 reported a voltammetric biosensor based on the chemically modified graphene paste with a limit of detection of 0.02 nM and a dynamic range of 0.02 -21.2 nM.…”

Section: Development Of Biosensors Based On Fc Intercalatorsmentioning

confidence: 99%

A Novel DNA Biosensor Using a Ferrocenyl Intercalator Applied to the Potential Detection of Human Population Biomarkers in Wastewater

Yang

d’Auriac

Goggins

et al. 2015

Environ. Sci. Technol.

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A new label-free electrochemical DNA (E-DNA) biosensor using a custom synthesized ferrocenyl (Fc) double-stranded DNA intercalator as a redox marker is presented. Single-stranded DNA (ssDNA) was co-immobilized on gold electrodes with 6-mecarpto-hexanol to control the surface density of the ssDNA probe, and hybridized with complementary DNA. The binding of the Fc intercalator to dsDNA was measured by differential pulse voltammetry. This new biosensor was optimized to allow the detection of single base pair mismatched sequences, able to detect as low as 10 pM target ssDNA with a dynamic range from 10 pM to 100 nM. DNA extracted from wastewater was analyzed by quantitative polymerase chain reaction targeting human-specific mitochondrial DNA (mtDNA). The aim of this approach is to enable the analysis of population biomarkers in wastewater for the evaluation of public health using wastewater-based epidemiology (WBE). The E-DNA biosensor was employed to detect human-specific mtDNA from wastewater before and after PCR amplification. The results demonstrate the feasibility of detecting human DNA biomarkers in wastewater using the developed biosensor, which may allow the further development of DNA population biomarkers for public health using WBE.

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Section: Development Of Biosensors Based On Fc Intercalatorsmentioning

confidence: 99%

A Novel DNA Biosensor Using a Ferrocenyl Intercalator Applied to the Potential Detection of Human Population Biomarkers in Wastewater

Yang

d’Auriac

Goggins

et al. 2015

Environ. Sci. Technol.

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“…The electrochemical signal that produced by probe hybridized with target DNA is used for qualitative and quantitative detection. Based on hybridization type, the electrochemical sensors were divided into two categories: heterogeneous hybridization (Cai et al, 2002 ;Wang 2006 ;Li et al, 2014 ;Song and Jin 2015 ;Ionescu et al, 2006) and homogeneous hybridization (Cui et al, 2014 ;Fan et al, 2011a ;Fan et al, 2011b ;Fan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…As a result, we had developed the non-immobilizing electrochemical DNA sensing strategy to overcome some shortcomings. This type of sensor avoided immobilizing probe DNA on electrode surface and hybridization event occurred in a homogeneous solution which made it have the high sensitivity (Cui et al, 2014 ;Fan et al, 2011b;Fan et al, 2011a ;Fan et al, 2010). However, the necessary modifications on probe DNA and electrode surface made the non-immobilizing electrochemical DNA sensors more complex than E-DNA sensor.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical DNA sensor without electrode pre-modification

Hong

Cheng

Wei

et al. 2017

Biosensors and Bioelectronics

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We present a non-modification electrochemical DNA sensing strategy, which used Potential-Assisted Au-S Deposition and a clamp-like DNA probe. The dual-hairpin probe DNA was tagged with a methylene blue (MB) at the 3' terminal and a thiol at the 5' terminal., Without being hybridized with target DNA, the loop of probe prevented the thiol from reaching the bare gold electrode surface with an applied potential., After hybridization with the target DNA, the probe' s loop-stem structure opened through two distinct and sequential events, which led to the formation of a triplex DNA structure. Then the thiol easily contacted with electrode and resulted in potential-assisted Au-S self-assembly. Electrochemical signals of MB were measured by differential pulse voltammetry (DPV) and used for target quantitative detection. This strategy offered a detection limit down to 2.3pM. and an inherently high specificity for detecting even single mismatch.

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“…Bacillus cereus (B. Cereus) is one of the most common bacteria found in water and foods causing food poisoning and hence food borne illnesses [1,2]. When the B. Cereus affects the stomach and intestine, symptoms such as nausea, vomiting, abnormal cramps and diarrhea are observed [3].…”

Section: Introductionmentioning

confidence: 99%

Direct Impedimetric Detection and Isolation of Bacillus Cereus using Modified Platinum Electrode

Roy¹,

Ramamurthy²

2017

J. Res. Updates Polym. Sci.

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This work proposes a technique for isolation of bacteria using magnetic nanoparticles. The magnetic nanoparticles that are prepared by a sol-gel method using citric acid and nano-ferrites are characterized for structural and morphology by X-ray diffraction (XRD) and Transmission electron microscope (TEM), respectively. The prepared nano-ferrites coated with poly vinyl alcohol (PVA) over platinum electrode are used for detection of Bacillus Cereus. The isolated bacterial cells from colloidal solution are treated with zinc ferrite for 12 h, and it is observed that the cells are coagulated with the nanoparticle when allowed to settle down. This is further filtered by different size of filter paper to get less than 0.1% of cells in water. Various electrochemical parameters like cyclovoltammetry (CV), sweep step function, Tafel plot, AC impedance are studied employing the modified platinum electrode. It is observed through CV graph that the peaks are formed at -0.25 V due to the oxidation of bacterial cells, which is further supported by sweep step function graph. Therefore, this is one of the economically efficient techniques to detect and isolate the Bacillus cereus from a colloidal solution.

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A new electrochemical biosensor for DNA detection based on molecular recognition and lead sulfide nanoparticles

Cited by 13 publications

References 26 publications

A Novel DNA Biosensor Using a Ferrocenyl Intercalator Applied to the Potential Detection of Human Population Biomarkers in Wastewater

A Novel DNA Biosensor Using a Ferrocenyl Intercalator Applied to the Potential Detection of Human Population Biomarkers in Wastewater

An electrochemical DNA sensor without electrode pre-modification

Direct Impedimetric Detection and Isolation of Bacillus Cereus using Modified Platinum Electrode

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