A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans

Raulf, Marie‐Kristin; Jordan, Daniela; Auer, Herbert; Warnecke, Jens M.; Lepenies, Bernd; Strübe, Christina

doi:10.1017/s0031182020002085

Cited by 11 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serology is a commonly used method for diagnosing Toxocara infection [ 34 ]. In this regard, the detection of specific antibodies against Toxocara can be done using ELISA or immunoblotting techniques [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Toxocara spp. in Pregnant Women: A Systematic Review and Meta-Analysis

Shayanfar,

Bahadory,

Taghipour

et al. 2024

Infectious Diseases in Obstetrics and Gynecology

View full text Add to dashboard Cite

Background. Toxocariasis is an important health problem caused by the parasitic species Toxocara canis (T. canis) and Toxocara cati (T. cati). Prevalence of toxocariasis in pregnant women as a vulnerable population is doubly important, and the aim of this study is to estimate the overall prevalence of toxocariasis infection in pregnant women according to the available reports. Methods. The present study followed the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) checklists. A systematic search was carried out in international scientific databases (Google Scholar, Web of Science, ScienceDirect, Scopus, and PubMed) between 1990 and 2023. The overall prevalence of parasitic infection was estimated with a random-effects model. All analyses (overall prevalence, heterogeneity, publication bias, and sensitivity analysis) were performed with comprehensive meta-analysis (V2.2, Bio stat) software. Results. Amid the final eleven included studies, based on the random-effects model, the estimation of the pooled prevalence of Toxocara spp. was 20.8% (95% CI, 9.8–38.7%). The association between the risk factors of toxocariasis and the prevalence of the disease was not statistically significant. Conclusions. In the present study, significant prevalence was reported; however, considering the limited number of studies, it seems that the actual prevalence of the disease is higher. Therefore, it seems necessary to monitor this health problem in pregnant women.

show abstract

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Toxocara spp. in Pregnant Women: A Systematic Review and Meta-Analysis

Shayanfar,

Bahadory,

Taghipour

et al. 2024

Infectious Diseases in Obstetrics and Gynecology

View full text Add to dashboard Cite

show abstract

“…Diagnosis of human toxocariasis is based on the detection of anti- Toxocara antibodies by serological methods such as ELISA and western blotting [ 12 , 35 ]. The main antigens currently employed for the diagnosis of human toxocariasis are TES antigens, but the glycan structures of TES antigens cause cross-reactivity in the serodiagnosis of human toxocariasis [ 16 , 26 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of Toxocara cati Improves Its Efficacy in the Diagnosis of Human Toxocariasis

Pouryousef

Sarkari

Alavi

et al. 2023

Journal of Tropical Medicine

View full text Add to dashboard Cite

Background. Toxocariasis is an important zoonotic infection, especially in tropical areas. One of the significant challenges in the serodiagnosis of human toxocariasis is the cross-reaction of Toxocara antigens with other parasites due to their relatively similar glycan structures. Removing the glycan structure from Toxocara excretory-secretory (TES) antigens may increase the efficacy of these antigens in the diagnosis of toxocariasis. The current study aimed to assess the efficacy of deglycosylated Toxocara cati excretory-secretory (dTES) antigens for the serodiagnosis of human toxocariasis. Methods. Toxocara ES antigens were prepared from T. cati second-stage larvae and deglycosylated using sodium hydroxide (NaOH). The TES antigens, along with the dTES antigens, were used in an ELISA as well as a western blotting system for the detection of anti-Toxocara antibodies. Sera samples collected from 30 confirmed cases of toxocariasis, 30 patients with other diseases, and 30 healthy subjects were evaluated by both systems. Results. The sensitivity of TES and dTES ELISA for the diagnosis of human toxocariasis was 96.67% (95% CI = 82.78–99.92) and 93.33% (95% CI = 77.93–99.18), respectively, while the specificity of dTES (88.33%; 95% CI = 77.43–95.18) increased significantly compared to the TES (80.00%; 95% CI = 67.67–89.22). The sensitivity of both antigens was 100% (95% CI = 88.43–100) by the western blotting system. Moreover, the specificity of TES and dTES antigens was 95% (95% CI = 86.08–98.96) and 98.33% (95% CI = 91.06–99.96), respectively, when using the western blotting system. Conclusion. Results of the current study indicate that the chemical removal of the glycan epitopes of T. cati ES antigens significantly reduces cross-reactivity rates with other parasitic infections. Considering the findings of the present study, the dTES antigens seem to be suitable antigens for the serodiagnosis of human toxocariasis.

show abstract

“…in pets are fundamentally based on the microscopic examination of faeces to find eggs and analyse their morphology, using different coprodiagnostic techniques such as direct smear, Kato-Katz, MacMaster, and Faust (flotationsedimentation), among others [22]. However, other diagnostic tests have greater sensitivity and specificity, such as serological tests, ELISA to detect anti-Toxocara IgG antibodies, and molecular techniques, such as polymerase chain reaction (PCR) and Western blot, that determine the larval TES antigen [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Toxocara cati Infection in Cats (Felis catus): A Systematic Review and Meta-Analysis

Bonilla-Aldana,

Espinosa-Nuñez,

Bonilla-Aldana

et al. 2024

Animals

View full text Add to dashboard Cite

Introduction: Toxocariasis is an infection caused in canines, felines, humans, and other vertebrates by species of the genus Toxocara, such as T. canis and T. cati. The embryonated eggs of these parasites are the primary means of acquiring the infection for both definitive hosts, dogs and cats, respectively, and for intermediates, such as humans and other vertebrates. When deposited on park soils, environmental contamination becomes a risk to environmental, human, and animal health. Objective: To determine the global prevalence of Toxocara cati in cats (Felis catus). Methods: A systematic review of the literature was carried out in six databases (Scopus, PubMed, ScienceDirect, SciELO and Google Scholar) to evaluate the global prevalence of Toxocara cati in cats, defined by coproparasitological, histological, and molecular techniques. A meta-analysis was performed using a random effects model to calculate pooled prevalence and 95% confidence intervals (95% CI). A two-tailed 5% alpha level was used for hypothesis testing. Results: Two hundred and eighty-nine studies were included. The global pooled prevalence of Toxocara cati in cats using coproparasitological methods was 17.0% (95.0% CI: 16.2–17.8%). In the subgroup analysis according to country, Nepal had the highest prevalence of T. cati infection (94.4%; 95% CI 89.7–99.2%). The pooled prevalence of T. cati infection by PCR in four studies was 4.9% (95.0% CI: 1.9–7.9%). Conclusions: This systematic review underscores the need for preventive action against toxocariasis due to its widespread prevalence. The interplay between animal and human health should be emphasised, necessitating measures like deworming cats, hygiene practices, and public education to mitigate risks. Safeguarding feline health can also reduce human transmission, benefiting both species.

show abstract

A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans

Abstract: A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans. Parasitology 1-8.

Cited by 11 publications

References 38 publications

Seroprevalence of Toxocara spp. in Pregnant Women: A Systematic Review and Meta-Analysis

Seroprevalence of Toxocara spp. in Pregnant Women: A Systematic Review and Meta-Analysis

Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of Toxocara cati Improves Its Efficacy in the Diagnosis of Human Toxocariasis

Toxocara cati Infection in Cats (Felis catus): A Systematic Review and Meta-Analysis

Contact Info

Product

Resources

About