A new enzymatic assay for selectively measuring conjugated bilirubin concentration in serum with use of bilirubin oxidase

Kurosaka, Keisuke; Senba, Shoji; Tsubota, Hiroyuki; Kondo, Hitoshi

doi:10.1016/s0009-8981(97)00194-0

Cited by 65 publications

(42 citation statements)

References 19 publications

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“…A similar result has also been reported 12,23 on human blood specimens using these methods. The inter-and intra-assay precision studies revealed that the vanadate oxidase method has comparable between-run and within-run CVs to those of the diazo method for both [Bt] and [Bd].…”

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confidence: 88%

“…1,9,11,12,16,17 In these methods, bilirubin oxidase catalyzes the oxidation of bilirubin to biliverdin, which causes the characteristic yellow absorption band for bilirubin to disappear. It has been suggested 9,11,12,16,17 that Bc can be exclusively detected by bilirubin oxidase methods. The vanadate oxidase method is based on oxidation of bilirubin to biliverdin, accomplished by utilization of vanadate as an oxidizing agent.…”

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confidence: 99%

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Comparison of the Vanadate Oxidase Method with the Diazo Method for Serum Bilirubin Determination in Dog, Monkey, and Rat

Ameri¹,

Schnaars²,

Sibley³

et al. 2011

J VET Diagn Invest

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Abstract. The most widely used method for bilirubin concentration determination is the diazo method, which measures the color of azobilirubin. The vanadate oxidase method is based on oxidation of bilirubin to biliverdin by vanadate. The objective of this study was to compare total and direct bilirubin concentration ([Bt] and [Bd], respectively) determined by the diazo and vanadate oxidase methods in pooled serum samples from dogs, monkeys, and rats spiked with panels of different concentrations of bilirubin standards. Pooled serum samples from 40 dogs, 40 monkeys, and 60 rats were spiked with either ditaurine conjugates of bilirubin or a standard reference material. The results obtained from both assays were compared using Deming regression analysis. The intra-and interassay precision, expressed as a percentage of the coefficient of variation (%CV), was determined for [Bt] and [Bd], and the mean percentage of recovery was calculated. The vanadate oxidase method displayed an excellent correlation (r 5 0.99-1.00) with the diazo method. Using Deming regression, there were minimal negative or positive constant and proportional biases for [Bt] and [Bd]. The precision studies revealed that the vanadate oxidase method has comparable between-run and within-run CVs to those of the diazo method. The recovery study demonstrated that the diazo method more closely approximates the expected values of [Bt]. In conclusion, the vanadate oxidase method is a simple and rapid method that can be employed as an alternative to the diazo method when interfering substances are present in the serum samples of dog, monkey, and rat.

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confidence: 88%

Section: <!?Show "Fnote_aff1"$^!"content-markup(/author-grp[1]/aff|/author-grp[1]/dept-list)>mentioning

confidence: 99%

Comparison of the Vanadate Oxidase Method with the Diazo Method for Serum Bilirubin Determination in Dog, Monkey, and Rat

Ameri¹,

Schnaars²,

Sibley³

et al. 2011

J VET Diagn Invest

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“…Bilirubin oxidase (BOD; EC 1.3.3.5) catalyzes the oxidation of bilirubin to biliverdin (2) and is used clinically to determine the levels of total and conjugated bilirubin in serum (3,12,14). It is distributed mainly in fungi, and BODs from Myrothecium verrucaria (20) and Trachyderma tsunodae K-2593 (6) have been purified and characterized.…”

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confidence: 99%

Bilirubin Oxidase Activity of Bacillus subtilis CotA

Sakasegawa

Ishikawa

Imamura

et al. 2006

Appl Environ Microbiol

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“…In addition, a modified Jendrassik-Grof method (J-G method) and spectrophotography 3,4 are also familiar methods to determine BR in clinical analysis. Several methods, such as the spectrophotometric method, 5,6 bilirubin oxidase enzymatic analysis, [7][8][9] fiber optic sensor, 10 chemiluminescence combine flow injection analysis [11][12][13] and capillary micellar electrokinetic chromatography 14 have been developed. However, using a fluorescent probe to determine BR has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorometric Determination of Total Bilirubin in Human Serum Samples Using Tetracycline-Eu3+

Bian

Zhang

Wang³

2010

ANAL. SCI.

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The measurement of total bilirubin in serum is one of the most frequently performed tests in clinical analysis. A new spectrofluorometric method was developed for the determination of trace amounts of total bilirubin. Using tetracycline-Eu 3+ as a fluorescent probe, bilirubin can remarkably reduce the fluorescence intensity of the tetracycline-Eu 3+ complex, and the reduced fluorescence intensity of Eu 3+ ion is in proportion to the concentration of bilirubin. The optimum conditions for the determination of bilirubin were also investigated. This method is simple, practical, and relatively free of interference from coexisting substances. It can be successfully applied to assess total bilirubin in serum samples. We also compared the developed method with a modified Jendrassik-Grof method in clinical analysis.

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A new enzymatic assay for selectively measuring conjugated bilirubin concentration in serum with use of bilirubin oxidase

Cited by 65 publications

References 19 publications

Comparison of the Vanadate Oxidase Method with the Diazo Method for Serum Bilirubin Determination in Dog, Monkey, and Rat

Comparison of the Vanadate Oxidase Method with the Diazo Method for Serum Bilirubin Determination in Dog, Monkey, and Rat

Bilirubin Oxidase Activity of Bacillus subtilis CotA

Spectrofluorometric Determination of Total Bilirubin in Human Serum Samples Using Tetracycline-Eu3+

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