A new fluorometric analysis of citric acid

Hori, Mayuko; Kometani, Tadahiko; Ueno, Hayao; Morimoto, Hiroshi

doi:10.1016/0006-2944(74)90094-5

Cited by 23 publications

(8 citation statements)

References 15 publications

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“…A sensitive and specific fluorimetric method for determination of citrate from raw milk samples (Hori et al, 1974) was applied with some modifications.…”

Section: Fluorimetric Determination Of Citratementioning

confidence: 99%

Relationship between concentration of citrate and ketone bodies in cow's milk

Baticz

Tömösközi

Vida

2002

Acta Veterinaria Hungarica

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The authors' hypothesis is that the members of the tricarboxylic acid cycle (TCA cycle) such as citrate decrease in association with increased ketone body formation. To prove this hypothesis the connection between ketone bodies and citrate formation of milk was studied. A fluorimetric method was used to determine citrate and a headspace sampling gas chromatographic (GC) method was developed for determination of ketone bodies. Under real conditions of milk sampling, transport and storage, preserved milk samples of 119 clinically healthy dairy cows obtained in the 48 hours after milking were investigated. A low level of acetoacetate (ACAC) was found in all samples. This fact can be explained by the spontaneous decarboxylation of acetoacetate during sample storage (previously decarboxylised acetoacetate = pdACAC) and, consequently, the majority of the amount of acetoacetate in the samples (AC+pdACAC) appeared in the measured acetone concentrations. Based on the measured acetone concentration of milk samples two groups were formed retrospectively: HA (high-acetone) group (n = 41) with an AC+pdACAC concentration of > 0.4 mmol/l and a LA (lowacetone) group (n = 78) with an AC+pdACAC level of ≤ 0.4 mmol/l. In the milk of cows of Group HA a positive correlation (r = +0.623) and linear connection between acetone (AC+pdACAC) and β-hydroxybutyrate (BOHB) levels was found [BOHB = 2.491 + 0.586 × (pdAC + ACAC)]. Furthermore, in this group a negative correlation between citrate and BOHB and AC+pdACAC was also established (r = -0.579). Focusing on the results of this group the authors found a significant drop of AC+pdACAC and citrate during the metabolically critical first 1-4 weeks of lactation. For this reason they suggest that simple, easy, automated methods (i.e. flow injection analysis, Fourier transformation infrared analysis) should be introduced for the simultaneous determination of acetone and citrate concentration in milk to make the evaluation of the energy status of highproducing dairy cows easier and more certain.

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“…A sensitive and specific fluorimetric method for determination of citrate from raw milk samples (Hori et al, 1974) was applied with some modifications.…”

Section: Fluorimetric Determination Of Citratementioning

confidence: 99%

Relationship between concentration of citrate and ketone bodies in cow's milk

Baticz

Tömösközi

Vida

2002

Acta Veterinaria Hungarica

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“…[9] Almost a hundred years later, fluorescent five-membered ring fused 2-pyridones were synthesized by reacting CA with D-penicillamine [10] and o -amino-thiophenol. [11] In the past decade, fluorescent carbon dots (CDs) have been widely developed through pyrolysis of CA with or without amine based molecules. [12, 13–15] These citrate-based CDs, when compared with traditional fluorescent agents, possess numerous advantages, including biocompatibility, straightforward synthesis, and strong fluorescence with adjustable emission spectra (from blue to NIR).…”

Section: Introductionmentioning

confidence: 99%

Citrate‐Based Fluorescent Biomaterials

Shan

Hsieh

Bai

et al. 2018

Adv Healthcare Materials

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Fluorescence imaging has emerged as a promising technique for monitoring and assessing various biologically relevant species in cells and organisms, driving the demand for effective fluorescent agents with good biocompatibility and high fluorescence performance. However, traditional fluorescent agents, such as quantum dots (QDs) and organic dyes, either suffer from toxicity concerns or poor fluorescence performance (e.g., low photobleaching-resistance). In this regard, citrate-based fluorescent biomaterials, which are synthesized from the natural and biocompatible precursor of citric acid (CA), have become competitive alternatives for fluorescence imaging owing to their biocompatibility, cost effectiveness, straightforward synthetic routes, flexible designability, as well as strong fluorescence with adjustable excitation/emission wavelengths. Accordingly, numerous citrate-based biomaterials, including carbon dots (CDs), biodegradable photoluminescent polymers (BPLPs), and small molecular fluorophores, have been developed and researched in the past few decades. This review discusses recent progress in the research and development of citrate-based fluorescent materials with emphasis on their design and synthesis considerations, material properties, fluorescence properties and mechanisms, as well as biomedical applications. It is expected that this review will provide an insightful discussion on the citrate-based fluorescent biomaterials, and lead to innovations for the next generation of fluorescent biomaterials and fluorescence-based biomedical technology.

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“…2 Later on CA was reacted with specic aliphatic and aromatic b-amino thiols to produce highly uorescent ve membered ring fused 2-pyridones. 3,4 Recently, many authors have reported synthesis of uorescent carbon and graphene dots prepared through pyrolysis of citric acid (CCD). These materials possess a number of advantages such as biocompatibility, straightforward synthesis and excitation dependent emission spectra but also have one important drawback i.e.…”

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confidence: 99%