It is well known that the mechanical properties of a steel plate depend on the anisotropy of the material and the rolling directions. This paper presents the results of the Charpy V-Notch (CVN) impact test for the ST, TL, TS, LS, LT, 45°, and SL directions in API 5L X52 pipelines with electric-resistance-welded (ERW) and seamless (SMLS) pipes. Charpy specimens were manufactured and tested according to the ASTM E23 standard in laboratory conditions. All possible directions in the pipe were tested. Three Charpy specimens were tested for each direction, for a total of 21 Charpy tests. Moreover, the microstructures, hardness, ductile and brittle areas, and fracture surfaces of the Charpy specimens are presented in this research. The results show that the Charpy energy values, hardness, and microstructures depend on the direction of the specimens. The Charpy values of the SMLS pipe are higher than those of the ERW pipe because of several metallurgical factors, such as grain size, non-metallic inclusions, delaminations, and microstructures.