A new FRET probe for ratiometric fluorescence detecting mitochondria-localized drug activation and imaging endogenous hydroxyl radicals in zebrafish

Deng, Tao; Wang, Xiaojuan; Wu, Shengjun; Hu, Shiyou; Liu, Wei; Chen, Tongkai; Yu, Zhiqiang; Xu, Qin; Liu, Fang

doi:10.1039/d0cc00382d

Cited by 43 publications

(22 citation statements)

References 33 publications

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“…Indeed, in the reported • OH probes containing amide groups, the amide was not the reaction site. 29,44 Moreover, singlet oxygen ( 1 O 2 ) will not interfere with the response of probe FDOCl-22 toward HOCl (Figures S12 and S13). These results indicated that the probes had good selectivity for HOCl.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In the present work, the insensitivity of FDOCl-21 (5 μM) and FDOCl-22 toward • OH may be because the experimental conditions that mimic the normal physiological conditions do not meet the requirements of harsh condition for the reaction between • OH and amide. Indeed, in the reported • OH probes containing amide groups, the amide was not the reaction site. , Moreover, singlet oxygen ( 1 O 2 ) will not interfere with the response of probe FDOCl-22 toward HOCl (Figures S12 and S13). These results indicated that the probes had good selectivity for HOCl.…”

Section: Resultsmentioning

confidence: 99%

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Dual-Modality Detection of Early-Stage Drug-Induced Acute Kidney Injury by an Activatable Probe

et al. 2020

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Early detection of drug-induced acute kidney injury (AKI) is crucial for effective treatment and prevention of further injury. It remains challenging, however, because of the lack of activatable indicators with multimodality imaging capability that could increase the accuracy of diagnosis by mutual verification. Herein, we report an activatable probe, FDOCl-22, that enabled dual-modality detection of the early-stage drug-induced AKI. FDOCl-22 was completely soluble in water and highly sensitive to hypochlorous acid (HOCl). Dramatic increases of both near-infrared (NIR) emission and absorption were observed after reaction with HOCl. A correlation between HOCl concentration and drug-induced AKI was established using FDOCl-22 as a tool. As a consequence, the HOCl-activated probe was able to detect the early-stage drug-induced AKI by dual-modality imaging, irrespective of the drug stimulation time or dosage, by combining NIR fluorescence and photoacoustic imaging.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Dual-Modality Detection of Early-Stage Drug-Induced Acute Kidney Injury by an Activatable Probe

et al. 2020

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“…So far, FRET fluorescent probes have been widely reported in recent years (16)(17)(18)(19)(20). FRET fluorescent probes not only have mature applications in the detection of metal ions (21), ROS (22) and RNS (23), but also can be used to sense biological macromolecules (e.g., proteins (24)(25)(26)(27), nucleic acids (28,29)), and even be used for the visualization of changes in the cell microenvironment and the monitoring of pathophysiological processes (30)(31)(32)(33)(34). There is no doubt that FRET probes are of great significance to biological research.…”

Section: Introductionmentioning

confidence: 99%

Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection^†

Zhu

Ouyang

et al. 2022

Photochem & Photobiology

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Fluorescent probes based on fluorescence resonance energy transfer (FRET) are highly promising for diverse bioapplications. The key to constructing FRET probes is to confine the donor and acceptor within a sufficiently close distance. However, the commonly used covalent linkage often requires elaborate design and complex organic synthesis, and sometimes causes changes in the fluorescence properties of the donor and acceptor. Inspired by the binding between small molecules and protein in nature, herein, we propose a proteinmediated strategy to fabricate FRET probe. In such proteinmediated FRET (P-FRET) probe, protein acts as a carrier to simultaneously confine donor and acceptor in its cavity. As a proof of concept, we use bovine serum albumin (BSA) as a model protein, coumarin derivative as a donor and hydroxyl radical (ÁOH)-responsive dye fluorescein as an acceptor. Through a series of investigations, including binding parameters, fluorescence properties and detection performance, we prove that the construction of P-FRET probe is simple and feasible and the detection is sensitive. Our P-FRET strategy will provide new insights for the design of FRET probes.

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“…Model organisms ranging from invertebrates, such as Caenorhabditis elegans (Beam et al 2012 ) and Drosophila melanogaster (Wang et al 2004 ), to vertebrates like Xenopus laevis and the zebrafish Danio rerio have all been studied with FCS (Shi et al 2009a , b ). The zebrafish, in particular, has greatly furthered the field of developmental biology with the use of FCS and other confocal-microscopy-related quantification technologies such as FCCS, Förster resonance energy transfer (FRET) and fluorescence recovery after photobleaching (FRAP) (Deng et al 2020 ; Wang et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo

Dawes

Soeller

Scholpp

2020

Histochem Cell Biol

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Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical technique that non-invasively resolves single molecules through recording the signal intensity at the femtolitre scale. However, recording the behaviour of these biomolecules using in vitro-based assays often fails to recapitulate the full range of variables in vivo that directly confer dynamics. Therefore, there has been an increasing interest in observing the state of these biomolecules within living organisms such as the zebrafish Danio rerio. In this review, we explore the advancements of FCS within the zebrafish and compare and contrast these findings to those found in vitro.

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A new FRET probe for ratiometric fluorescence detecting mitochondria-localized drug activation and imaging endogenous hydroxyl radicals in zebrafish

Abstract: A new FRET probe has been prepared and successfully used for imaging hydroxyl radicals generated by drug activation and endogenous hydroxyl radicals in zebrafish.

Cited by 43 publications

References 33 publications

Dual-Modality Detection of Early-Stage Drug-Induced Acute Kidney Injury by an Activatable Probe

Dual-Modality Detection of Early-Stage Drug-Induced Acute Kidney Injury by an Activatable Probe

Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection^†

Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo

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A new FRET probe for ratiometric fluorescence detecting mitochondria-localized drug activation and imaging endogenous hydroxyl radicals in zebrafish

Abstract: A new FRET probe has been prepared and successfully used for imaging hydroxyl radicals generated by drug activation and endogenous hydroxyl radicals in zebrafish.

Cited by 43 publications

References 33 publications

Dual-Modality Detection of Early-Stage Drug-Induced Acute Kidney Injury by an Activatable Probe

Dual-Modality Detection of Early-Stage Drug-Induced Acute Kidney Injury by an Activatable Probe

Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection†

Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo

Contact Info

Product

Resources

About

Protein‐Mediated Fluorescence Resonance Energy Transfer (P‐FRET) Probe: Fabrication and Hydroxyl Radical Detection^†